Bacteriophage-based control of biogenic hydrogen sulphide produced by multidrug resistant Salmonella enterica in synthetic sewage

Lytics broadcasting system: A novel approach to disseminate bacteriophages for disinfection and biogenic hydrogen sulphide removal tested in synthetic sewage

“…The culture was subcultured in Salmonella - Shigella Agar (SS Agar, HiMedia, India) and stored at 4 °C throughout the experiment. Bacteriophage φPh_Se01 previously reported for its ability to reduce biogenic H 2 S produced by S. enterica was employed for the disinfection experiments [ 25 ]. The phage was stored and maintained at 4 °C for immediate use and at −80 °C for long term storage.…”

“…4 C. The sample was mixed using magnetic beads at ∼200 rpm and the flasks were sealed with parafilm to ensure adequate H 2 S production. Samples were collected beforehand from both control and test sets in order to determine the number of bacteria at 0 h. The experimental setups were incubated at room temperature for a period of 48 h. Following the incubation, a 5% lead acetate strip (23 × 4 cm) was introduced into the flasks for a period of 2 h to determine the production of H 2 S in both setups; the difference in the integrated density was quantified using Image J as described in the previous studies [ 25 ]. Subsequently, 1 mL of the sample was also collected from each set up to determine the bacterial cell viability count and phage titre.…”

“…One of the major sources of foul odour is hydrogen sulfide produced in wastewater by sulphate reducing bacteria (SRBs). Selective elimination of infectious organisms and biogenic malodour mitigation could be effectively employed in wastewater treatment facilities by phages as discussed in our previous study [ 25 ]. An added advantage is that phages can minimize the selective pressure imparted by residual antibiotics to AMR bacteria and their subsequent propagation in wastewater.…”

A Review of the State of the Art of Biomethane Production: Recent Advancements and Integration of Renewable Energies

“…The removal of siloxanes is certainly of utmost importance, but other substances can seriously affect the quality of the biogas produced, limiting its further use. Salim et al [104] investigated the effect of adopting sulfate-reducing bacteria (SRB) in a simulated synthetic sewage reactor for the efficient removal of H 2 S. The bacteriophages families were provided by the multidrug-resistant Salmonella enterica clinical strain and the results showed a H 2 S removal ranging from 60% to 70%. The importance of this novel solution lies in the fact that many existing solutions are very expensive and cannot be applied in all cases, especially in less developed countries.…”

Expression profile, transcriptional and post‐transcriptional regulation of genes involved in hydrogen sulphide metabolism connecting the balance between development and stress adaptation in plants: a data‐mining bioinformatics approach

“…Hydrogen sulphide (H 2 S) is a combustible, colourless, chalcogen hydride gas that smells like rotten eggs. It is also produced by microbes during microbial decomposition of organic waste under anaerobic conditions, such as in swamps and sewers (Salim et al 2021). The initial concept of H 2 S as a toxic agent in biological systems was later refuted when it was discovered to be involved in signalling in brain cells of animals (Goodwin et al 1989;Abe & Kimura, 1996).…”