Bacteriology of Amniotic Fluid in Women With Suspected Cervical Insufficiency

Bujold, Emmanuel; Morency, Anne‐Maude; Rallu, Fabien; Ferland, Sylvie; Têtu, Amélie; Duperron, Louise; Audibert, François; Laferrière, Céline

doi:10.1016/s1701-2163(16)32967-x

Cited by 72 publications

(40 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Conflicting associations between mollicutes and preterm birth have had a long history, not only related to population tested, but also to testing itself (culture vs PCR) (5,7,8,9,10,11,12,16,20,21,22,23,24,25,26). We used PCR probes that can separate M hominis , M genitalium , and U parvum from U urealyticum (18).…”

Section: Discussionmentioning

confidence: 99%

“…A UTI diagnosis was based on results of a routine culture of a first-void, clean-catch urine specimen obtained during gestation weeks 18 to 28 for asymptomatic bacteruria, or at the time of symptoms or signs of UTI. Specimen storage, DNA extraction, and PCR hybridization probes were as reported elsewhere (15,16,17,18), with details available on request.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiologic Factors and Urogenital Infections Associated With Preterm Birth in a Midwestern U.S. Population

Agger

Siddiqui

Lovrich

et al. 2014

Obstetrics &Amp; Gynecology

View full text Add to dashboard Cite

Objective To correlate epidemiologic factors with urogenital infections associated with preterm birth. Methods Pregnant women were sequentially included from four Wisconsin cohorts: large urban, midsize urban, small city, and rural city. Demographic, clinical, and current pregnancy data were collected. Cervical and urine specimens were analyzed by microscopy, culture, and polymerase chain reaction for potential pathogens. Results Six hundred seventy-six women were evaluated. Fifty-four (8.0%) had preterm birth: 12.1% (19/157) large urban, 8.8% (15/170) midsize urban, 9.4% (16/171) small city, and 2.3% (4/178) rural city. Associated host factors and infections varied significantly among sites. Urogenital infection rates, especially Mycoplasma hominis and Ureaplasma parvum, were highest at the large urban site. Large urban site, minority ethnicity, multiple infections, and certain historical factors were associated with preterm birth by univariable analysis. By multivariable analysis, preterm birth was associated with prior preterm birth (adjusted odds ratio [aOR] 2.76, 95% confidence interval [CI] 1.27–6.02) and urinary tract infection (aOR 2.62, 95% CI 1.32–519), and negatively associated with provider-assessed good health (aOR 0.42, 95% CI 0.23–0.76) and group B streptococcal infection treatment (surrogate for healthcare utilization) (aOR 0.38, 95% CI 0.15–.99). Risk and protective factors were similar for women with birth at < 35 weeks, and additionally associated with M hominis (aOR 3.6, 95% CI 1.4–9.7). Conclusion These measured differences between sites are consistent with observations that link epidemiologic factors, both environmental and genetic, with minimally pathogenic vaginal bacteria, inducing preterm birth, especially at less than 35 weeks of gestation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Epidemiologic Factors and Urogenital Infections Associated With Preterm Birth in a Midwestern U.S. Population

Agger

Siddiqui

Lovrich

et al. 2014

Obstetrics &Amp; Gynecology

View full text Add to dashboard Cite

show abstract

“…[8] We have also employed a combination of broad-range and specific PCR assays for bacteria and fungi, and have demonstrated that the combination of culture and molecular methods allows improved detection of MIAC. [16, 17] Importantly, an intrauterine inflammatory response is associated with the presence of microbial DNA in the amniotic fluid, even in the settings of a negative culture.…”

Section: Discussionmentioning

confidence: 99%

Microbial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses

DiGiulio¹,

Gervasi

Romero³

et al. 2010

Journal of Perinatal Medicine

View full text Add to dashboard Cite

Objective: Microbial invasion of the amniotic cavity (MIAC) has been detected in women with preterm labor, preterm prelabor rupture of membranes (PROM), and in patients at term with PROM or in spontaneous labor. Intrauterine infection is recognized as a potential cause of fetal growth restriction; yet, the frequency of MIAC in pregnancies with small-for-gestational-age (SGA) fetuses is unknown. The aim of this study was to determine the frequency, diver- sity and relative abundance of microbes in amniotic fluid (AF) of women with an SGA neonate using a combination of culture and molecular methods. Method: AF from 52 subjects with an SGA neonate was analyzed with both cultivation and molecular methods in a retrospective cohort study. Broad-range and group-specific PCR assays targeted small subunit rDNA, or other gene sequences, from bacteria, fungi and archaea. Results of microbiologic studies were correlated with indices of the host inflammatory response. Results: 1) All AF samples (ns52) were negative for microorganisms based on cultivation techniques, whereas 6% (3/52) were positive based on PCR; and 2) intra-amniotic inflammation was detected in one of the three patients with a positive PCR result, as compared with three patients (6.1%) of the 49 with both a negative culture and a negative PCR (Ps0.2). Conclusion: MIAC is detected by PCR in some patients with an SGA fetus who were not in labor at the time of AF collection.

show abstract

“…82 The presence of intra-amniotic inflammation (both microbial and sterile) is associated with adverse pregnancy outcomes 35,83–91 in patients presenting with preterm labor and intact membranes, 92–108 preterm pre-labor rupture of the membranes (PPROM), 109–114 a sonographic short cervix, 60,62,82,115 and cervical insufficiency. 58,59,64,116,117 Thus, we and others have explored the behavior of cytokines and other inflammatory-related markers in amniotic 82,100,106–108,118–128 or vaginal 129–140 fluid.…”

Section: | Introductionmentioning

confidence: 99%

The cytokine network in women with an asymptomatic short cervix and the risk of preterm delivery

Tarca

Fitzgerald

Chaemsaithong

et al. 2017

American J Rep Immunol

View full text Add to dashboard Cite

ProblemTo characterize the amniotic fluid (AF) inflammatory-related protein (IRP) network in patients with a sonographic short cervix (SCx) and to determine its relation to early preterm delivery (ePTD).Method of studyA retrospective cohort study included women with a SCx (≤25 mm; n=223) who had amniocentesis and were classified according to gestational age (GA) at diagnosis and delivery (ePTD <32 weeks of gestation).Results(i) In women with a SCx ≤ 22 1/7 weeks, the concentration of most IRPs increased as the cervix shortened; those with ePTD had a higher rate of increase in MIP-1α, MCP-1, and IL-6 concentrations than those delivering later; and (ii) the concentration of most IRPs and the correlation between several IRP pairs were higher in the ePTD group than for those delivering later.ConclusionWomen with a SCx at 16–22 1/7 weeks have a unique AF cytokine network that correlates with cervical length at diagnosis and GA at delivery. This network may aid in predicting ePTD.

show abstract

Bacteriology of Amniotic Fluid in Women With Suspected Cervical Insufficiency

Cited by 72 publications

References 31 publications

Epidemiologic Factors and Urogenital Infections Associated With Preterm Birth in a Midwestern U.S. Population

Epidemiologic Factors and Urogenital Infections Associated With Preterm Birth in a Midwestern U.S. Population

Microbial invasion of the amniotic cavity in pregnancies with small-for-gestational-age fetuses

The cytokine network in women with an asymptomatic short cervix and the risk of preterm delivery

Contact Info

Product

Resources

About