Bacteriology and antibiotic sensitivity of tonsillar diseases in Chinese children

Wang, Qian; Du, Jingdong; Chen, Jie; Ouyang, Hong; Luo, Renzhong; Li, Wei

doi:10.1007/s00405-017-4603-y

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…Despite this, much of current clinical practice and decision making is based on the ndings of these culture based studies. The ndings of this study are in keeping with a growing body of evidence that supports a polymicrobial community that is exposed to various aerobic-anaerobic environments on the surface and crypts of tonsils [31][32][33][34] . Finally, the described methodology in this protocol provides a strong foundation for future research in this area and in other human tissue samples prepared using the same techniques.…”

Section: Comparisons Of Sampling Methodologies In Tonsillar Hyperplasiasupporting

confidence: 82%

Fluorescence in Situ Hybridisation in Carnoy’s Fixed Tonsil Tissue

Clark

Waldvogel-Thurlow

Mackenzie

et al. 2022

Preprint

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Fluorescence in situ hybridisation (FISH) is a powerful molecular technique that enables direct visualisation of specific bacterial species. Few studies have established FISH protocols for tonsil tissue in Carnoy’s fixative, accordingly limiting its application to investigate the pathogenesis of tonsillar hyperplasia. Tonsil tissue from 24 children undergoing tonsillectomy for either recurrent tonsillitis or sleep-disordered breathing were obtained during a previous study. The specificity of each of the five FISH probes (Fusobacterium spp., Bacteroides spp., Streptococcus spp., Haemophilus influenzae and Pseudomonas spp.) were successfully optimised using pure and mixed bacterial isolates, and in Carnoy’s fixed tonsil tissue. Bacteroides spp. were present in 100% of patients with microcolonies. In comparison, the prevalence of Fusobacterium spp. was 93.8%, Streptococcus spp. 85.7%, H. influenzae 82.35% and Pseudomonas spp. 76.5%. Notable differences in the organisation of bacterial taxa within a single microcolony were also observed. This is the first study to establish a robust FISH protocol identifying multiple aerobic and anaerobic bacteria in Carnoy’s fixed tonsil tissue. This protocol provides a strong foundation for combining histological and microbiological analyses of Carnoy’s fixed tonsil samples. It may also have important implications on the analysis of microorganisms in other human tissues prepared using the same techniques.

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Section: Comparisons Of Sampling Methodologies In Tonsillar Hyperplasiasupporting

confidence: 82%

Fluorescence in Situ Hybridisation in Carnoy’s Fixed Tonsil Tissue

Clark

Waldvogel-Thurlow

Mackenzie

et al. 2022

Preprint

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“…However, using PICRUSt2 could provide new microbial insights to search for potential biomarkers or treatment targets for CT or TH patients ( 27 ). Moreover, given that most of the identified differential genera in our study were also previously identified between CT and TH patients using swab samples ( 7 – 9 ), we believe that our results provide valuable information for further studies to use, especially the bacteria we found to be related to CT versus TH and core versus surface tissues. Longitudinal studies that are focused on the metagenome and that use information on factors that influence microbiota, such as antibiotic exposure and eating habits, will provide further understanding on tonsillar microbiota.…”

Section: Discussionsupporting

confidence: 69%

“…One study with recurrent tonsillitis (RT) participants showed a high relative abundance of Parvimonas , while patients with obstructive sleep apnea (OSA) had a high relative abundance of Haemophilus and Capnocytophaga ( 8 ). Other studies have also identified that patients with OSA or TH have a higher relative abundance of Haemophilus ( 7 ) and Streptococcus ( 12 ) than RT patients. Moreover, the main species of Moraxella ( 13 ) and Neisseria ( 9 ) have also proven to be positively correlated with the occurrence of TH in previous research.…”

Section: Discussionmentioning

confidence: 96%

“…So far, only a few microbiota studies have been performed on tonsils, especially tonsillar tissues, although they play an essential role in the human immune system and encounter numerous microorganisms all the time (1,2,(6)(7)(8). Most of these studies were carried out using in vitro cultures of tonsillar bacteria (6,7), but the culturing methods mostly ignored the less abundant or unculturable bacteria (6,8). Other studies have used 16S rRNA gene sequencing with tonsillar swab samples, only considering surface microbiota (8)(9)(10)(11).…”

mentioning

confidence: 99%

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Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy

Hammarstedt‐Nordenvall

Jangard

et al. 2021

mSystems

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Chronic tonsillitis (CT) and tonsillar hypertrophy (TH) are common tonsillar diseases that are related to infection and inflammation. Little is known about tonsillar microbiota and its role in CT and TH. This study aims to identify palatine tonsillar microbiota both on the surface and in the core tissues of CT and TH patients. In total, 22 palatine tonsils were removed and collected from CT and TH patients who underwent surgery. The surface and core microbiota in the tonsils of CT and TH patients were compared using 16S rRNA gene sequencing of V3-V4 regions. Differential tonsillar microbiotas were found in the CT versus TH patients and surface versus core tissues. Further, a higher relative abundance of bacterial genera, including Haemophilus, Streptococcus, Neisseria, Capnocytophaga, Kingella, Moraxella, and Lachnospiraceae [G-2] in patients with TH and Dialister, Parvimonas, Bacteroidales [G-2], Aggregatibacter, and Atopobium in patients with CT, was observed. Of these, the differential genera of Dialister, Parvimonas, and Neisseria served as key factors in the tonsillar microbiota network. Notably, four representable tonsillar microbial types were identified, with one, consisting of a higher abundance of Haemophilus and Neisseria, exclusively detected in the TH patients. This study analyzed the different tonsillar microbiota from the surface and core tissues of CT and TH patients. Several bacteria and various microbial types related to CT and TH were identified, along with potential bacterial networks and related immune pathways. IMPORTANCE The human microbiota has been shown to be functionally connected to infectious and inflammation-related diseases. So far, only limited studies had been performed on tonsillar microbiota, although tonsils play an essential role in the human immune defense system and encountered numerous microorganisms. Our work presented different tonsillar microbiota from surface and core tissues of chronic tonsillitis (CT) and tonsillar hypertrophy (TH) patients. Notably, one tonsillar microbiota type, which contains a higher abundance of Haemophilus and Neisseria, was only detected in the TH patients. Furthermore, certain bacteria, such as Haemophilus, Neisseria, Dialister, and Parvimonas, may serve as microbial biomarkers to discriminate CT patients from TH patients. These data provide important microbiota data in the tonsillar research area and are highly useful for researchers both in the oral microbiome field and clinical field.

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“…bacteria within the tonsil parenchyma were mainly attached to the epithelial surface of the crypt and barely invaded the basal layer. For the convenience of sample collecting, microbial samples taken from the tonsil surface were widely used in previous studies of tonsillar microbiota (Khadilkar and Ankle, 2016;Wang et al, 2017;Galli et al, 2020;Katkowska et al, 2020).…”

Section: Comparison Of the Tonsil Surface Microbiota With The Internal Tonsil Tissue Microbiotamentioning

confidence: 99%

A Correlation Study of the Microbiota Between Oral Cavity and Tonsils in Children With Tonsillar Hypertrophy

Tian

Shi

et al. 2022

Front. Cell. Infect. Microbiol.

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Tonsillar hypertrophy is a common disease in 3-to-6-year-old children, which may cause serve symptoms like airway obstruction. Microbiological factors play an important role in the etiology of tonsillar hypertrophy. As the starting point of digestive and respiratory tracts, the microbial composition of the oral cavity is not only unique but also closely related to the resident microbiota in other body sites. Here we reported a correlation study of the microbiota between oral cavity and tonsils in children with tonsillar hypertrophy. Saliva, supragingival plaque, and wiped samples from the tonsil surface were collected from both tonsillar hypertrophy patients and participants with healthy tonsils and were then analyzed using Illumina Miseq Sequencing of the 16S rRNA gene. In the tonsillar hypertrophic state, more genera were detected on the tonsil surface than in the tonsil parenchyma, with more intra-microbiota correlations. When tonsillar hypertrophy occurred, both the oral cavity and tonsil surface endured microbiome shift with increased genera category and more active bacterial interactions. Over half of the newly detected genera from the tonsillar hypertrophic state were associated with infection and inflammation process or exhibited antibiotic-resistant characters. Of each individual, the microbial composition and structure of saliva seemed more similar to that of the tonsil surface, compared with the supragingival plaque. In salivary microbiota, genus Johnsonella might be relative with the healthy state of tonsils, while Pseudoxanthomonas might be relative with tonsillar hypertrophy. Our study supported the link between oral microbiota with the healthy and hypertrophic states of tonsils and may provide new directions for future researches in the specific role of oral microbiota in the etiology of tonsil diseases.

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Bacteriology and antibiotic sensitivity of tonsillar diseases in Chinese children

Cited by 14 publications

References 18 publications

Fluorescence in Situ Hybridisation in Carnoy’s Fixed Tonsil Tissue

Fluorescence in Situ Hybridisation in Carnoy’s Fixed Tonsil Tissue

Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy

A Correlation Study of the Microbiota Between Oral Cavity and Tonsils in Children With Tonsillar Hypertrophy

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