Bacteriological study of surgical site infections in a tertiary care hospital at Miraj, Maharashtra state, India

Bhave, Poorva P.; Kartikeyan, Sundaram; Ramteerthakar, M; Patil, Nilima R.

doi:10.18203/2320-6012.ijrms20161922

Cited by 4 publications

(5 citation statements)

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“…In a study conducted in a tertiary care hospital in southern India (Bangalore) 7.8% of all SSIs were culture negative [ 21 ]. Similarly, in a study conducted in a medical college in western India (Maharashtra), out of 196 pus samples taken from SSI patients 5.4% were negative in culture [ 22 ]. In those cases, although there was the presence of bacteria in some samples, other factors plays a crucial role in preventing their growth on culture plate such as, sample collection after the commencement of antibiotics, presence of viable but non-culturable (VBNC) bacteria, presence of biofilm producing bacteria or fastidious bacteria in the sample [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, in a study conducted in a medical college in western India (Maharashtra), out of 196 pus samples taken from SSI patients 5.4% were negative in culture [ 22 ]. In those cases, although there was the presence of bacteria in some samples, other factors plays a crucial role in preventing their growth on culture plate such as, sample collection after the commencement of antibiotics, presence of viable but non-culturable (VBNC) bacteria, presence of biofilm producing bacteria or fastidious bacteria in the sample [ 21 , 22 ]. If the laboratory condition is not suitable for the growth of anaerobic bacteria and the unculturable bacteria in samples it can also leads to no growth in culture even though Gram stain was positive.…”

Section: Discussionmentioning

confidence: 99%

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Identification of population of bacteria from culture negative surgical site infection patients using molecular tool

et al. 2021

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Background Managing surgical site infections, with negative culture report in routine diagnosis is a common dilemma in microbiology accounting more than 30% worldwide. The present study attempted to identify the presence of bacterial spp. if any in wound aspirates/swabs of culture negative surgical site infections of hospitalised patients using molecular tools. Methods Ninety-seven patients with post-operative SSI whose wound swabs/aspirate were negative in the conventional aerobic culture after 72 h of incubation were analysed by 16S rRNA gene specific broad range PCR. The amplified DNA fragments were sequenced by Sanger DNA sequencing method and homology of the sequence were matched using NCBI BLAST (NCBI, USA) Results Of the 97 patients, 16S rRNA based broad range PCR assay could identify the presence of bacterial pathogen in 53(54.63%) cases, of which 29 isolates were supposed to be of viable but non-culturable bacteria (VBNC), 07 were of obligatory anaerobes and 13 were of unculturable bacteria, 04 were with poly bacterial infections. Conclusions Our study highlights the usefulness of PCR assay in detecting the presence of any VBNC, anaerobes and unculturable bacteria in SSI patients regardless of how well the bacteria may or may not grow in culture. Measures should be taken to use anaerobic culture system and PCR diagnosis along with conventional culture to detect the VBNC and unculturable bacteria where Gram stain is positive for better patient care.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of population of bacteria from culture negative surgical site infection patients using molecular tool

et al. 2021

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“…(17.1%) were the most frequently isolated microorganisms from SSIs and were associated with a higher incidence of antibiotic resistance (64.9%) in ICU patients. Nonetheless, in another study by Bhave et al (2017) 36 in India, overall frequency of SSI was 6.17% where the most common isolates were Staphylococcus aureus, coagulase negative Staphylococci (CONS), E. coli and Pseudomonas aeruginosa. There are some other limitations to be mentioned to this study.…”

Section: Discussionmentioning

confidence: 97%

<p>Care Bundle Approach to Reduce Surgical Site Infections in Acute Surgical Intensive Care Unit, Cairo, Egypt</p>

Wassef¹,

Mukhtar²,

Nabil³

et al. 2020

IDR

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Introduction: Surgical site infections (SSIs) are one of the most frequently reported hospital acquired infections associated with significant spread of antibiotic resistance. Purpose: We aimed to evaluate a bundle-based approach in reducing SSI at acute surgical intensive care unit of the Emergency Hospital of Cairo University. Patients and Methods: Our prospective study ran from March 2018 to February 2019 and used risk assessment. The study was divided into three phases. Phase I: (pre-bundle phase) for 5 months; data collection, active surveillance of the SSIs, screening for OXA-48 producing Enterobacteriaceae and multidrug resistant Acinetobacter baumannii colonizers using Chrom agars were carried out. Phase II: (bundle-implementation) a 6-S bundle approach included education, training and postoperative bathing with Chlorhexidine Gluconate in collaboration with the infection control team. Finally, Phase III: (post-implementation) for estimation of compliance, rates of colonization, and infection. Results: Phase I encompassed 177 patients, while Phase III included 93 patients. A significant reduction of colonization from 24% to 15% (p<0.001) was observed. Similarly, a decrease of SSI from 27% to 15% (p=0.02) was noticed. A logistic regression was performed to adjust for confounding in the implementation of the bundle and we found a 70% reduction of SSI odd's ratio (OR's ratio = 0.3) confidence interval (95% CI 0.14-0.6) with significant Apache II (p=0.04), type of wound; type II (p=0.002), type III (p=0.001) and duration of surgery (p=0.04) as independent risk factors for SSI. Klebsiella pneumoniae was the most prevalent organism during phase I (34.7%). On the other hand, A. baumannii was the commonest organism to be isolated during phase III with (38.5%) preceding K. pneumoniae (30%). Conclusion: Our study demonstrated that the implementation of a multidisciplinary bundle containing evidence-based interventions was associated with a significant reduction of colonization and SSIs and was met with staff approval and acceptable compliance.

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“…[22] Forty seven percent of these patients require more than 14 days in the hospital compared with 5.9% of patients without a major infection. [23] As a result, infectious complications often increase the cost of care. [24] However, infectious complications can be reduced with many simple interventions, by risk factor modification preoperatively to postoperative risk factor vigilance in the ICU.…”

Section: Table 2: Antibiotic Susceptibility Pattern Discussionmentioning

confidence: 99%

Frequency and Antibiotic Susceptibility of Acinetobacter Baumannii in Surgical site Infections at Rawalpindi Institute of Cardiology

Ali¹,

Hussain²,

Kayani³

2020

JCCI

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Acinetobacter is an important nosocomial pathogen to cause infections such as pneumonia, bloodstream infections, meningitis, wound and surgical site infections including flesh eating bacterium necrotizing fasciitis and urinary tract Infections. A. baumannii can be spread through direct contact with surfaces, objects and the skin of contaminated persons. Acinetobactter has acquired resistance to most of the antibiotics and can play havoc in hospital environment due to its ability to survive desiccation and in moist environment in the presence of disinfectants. Currently, there are 32 genospecies of Acinetobacter known. Among these, A. baumannii is the most important in the clinical context, since it is the most frequently isolated in nosocomial infections and the one associated with the highest mortality rate. Objective To find out the frequency of A. baumannii causing surgical site infections after cardiac surgery and best antibiotic options for its treatment in patients admitted in Rawalpindi Institute of Cardiology. Materials and Methods A retrospective study was carried out to find out bacterial cultures in admitted patients at Rawalpindi Institute of Cardiology with surgical site infections from July 2017 to August 2018. A total of 405 pus specimens collected from patients with suspected surgical site infections were tested for bacterial culture and antimicrobial susceptibility test. Patient profile was noted from centralized hospital data. Results Out of total 405 specimens 13 were culture positive for A. baumannii (3.2%). Ten isolates were Pan Drug Resistant (PDR). Conclusion A. baumannii infection although infrequent in our set up is mostly pan resistant. Infection control practices & surveillance are important to avoid its spread in hospitalized patients.

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Bacteriological study of surgical site infections in a tertiary care hospital at Miraj, Maharashtra state, India

Cited by 4 publications

References 9 publications

Identification of population of bacteria from culture negative surgical site infection patients using molecular tool

Identification of population of bacteria from culture negative surgical site infection patients using molecular tool

<p>Care Bundle Approach to Reduce Surgical Site Infections in Acute Surgical Intensive Care Unit, Cairo, Egypt</p>

Frequency and Antibiotic Susceptibility of Acinetobacter Baumannii in Surgical site Infections at Rawalpindi Institute of Cardiology

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