Bacteriological Studies of Aureomycin

Price, Clifford W.; Randall, William A.; Welch, Henry

doi:10.1111/j.1749-6632.1948.tb27264.x

Cited by 40 publications

(9 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In studies on the development of resistance by the microbial flora, preliminary work was done to determine the effect of the culture medium and the temperature of incubation on the stability of the antibiotic. Price, Randall and Welch (12) have demonstrated that at 37" C., CTC was unstable in water and that this instability was increased in the presence of a variety of compounds and media. Since CTC was the least stable of the 3 tetracyclines, it was chosen for the determination of a desirable incubation temperature and medium.…”

Section: Results a N D Discussionmentioning

confidence: 99%

See 1 more Smart Citation

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINES^a,^b

Walker

Ayres

1958

Journal of Food Science

View full text Add to dashboard Cite

Many antibiotics have been investigated as possible agents for prolonging storage life of perishable foods ; chlortetracycline ( CTC) and oxy-tetracycline (OTC) were found acceptable for this purpose. These tetracyclines have received the approval of the United States Food and Drug Administration for use on poultry but with the stipulation that residuals not exceed 7 p.p.m. in the raw tissue and that no residual be present at the time the food is to be consumed.The tetracyclines retard the growth of the microflora for a time but after several days of storage the number of organisms increases and spoilage results. Subsequent growth of organisms on carcasses treated with tetracyclines could result from the development of an antibiotic resistant population or from a decrease in the activity of antibiotic in tissue to such a level that the organisms were no longer inhibited. An increase in the resistance of organisms growing on chicken processed in the presence of CTC as compared to poultry processed in the absence of CTC has been reported by Ng et al. (11).The purpose of this work was to determine the residuals of CTC, OTC and tetracycline (TC) remaining in raw poultry tissue after treatment with concentrations of 10 pg./ml. and 30 pg./ml. of the respective antibiotics and also to determine the effect of the residual antibiotics on the resistance of organisms causing spoilage of poultry. MATERIALS A N D METHODSThighs and backs of chickens were soaked for 10 or for 120 minutes in aqueous solutions containing 10 or 30 pg./../ml. of CTC, O T C or TC. Only thighs were used for determination of residual antibiotics in deep or subsurface tissue. The pieces of chicken were packaged in polyethylene bags and stored at 4" C. In addition, samples of antibiotic solutions used for treating the pieces of chicken were stored at 4" C. and were assayed for any decrease in concentration of antibiotic.Antibiotic concentrations were determined by microbiological assay using the pad plate method described by Grady and Williams (7). Duplicate determinations of the standard curve and of the unknown samples were made on each plate. Comparison of the average zone diameters of the unknown samples with those of the standard curve indicated the quantity of residual antibiotic. For the assay, 150 ml. of brain heart infusion agar inoculated with spores of Bacillus cereus var. mycoides (ATCC 9634) was poured into a pyrex utility dish (13% x 8% x 1% in.). The spore suspension generally was of such concentration that 1 ml. of spore suspension per 100 ml. agar gave satisfactory results.

show abstract

Section: Results a N D Discussionmentioning

confidence: 99%

“…Price, Randall and Welch (12) have demonstrated that at 37" C., CTC was unstable in water and that this instability was increased in the presence of a variety of compounds and media. Since CTC was the least stable of the 3 tetracyclines, it was chosen for the determination of a desirable incubation temperature and medium.…”

Section: Materials a N D Methodsmentioning

confidence: 97%

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINES^a,^b

Walker

Ayres

1958

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…This possib:lity is in accord with the data presented by Chandler and Bliss (1948), Tompsett, Shultz, and McDermott (1947), and Rammelkamp and Kiefer (1943), who showed that body fluids are capable of producing an inhibitory effect on antibiotic activity. Burkman, Henry, and Housewright (1947) and Price, Randall, and Welch (1948) also showed that the presence of specific inorganic salts and certain amino acids tend to decrease antibiotic activity. Inhibition of this type could occur in milk, because it is a body fluid containing some of the above-mentioned substances.…”

Section: Resultsmentioning

confidence: 97%

Antibiotic Sensitivities of Organisms Isolated from Mastitic and Nonmastitic Mammary Secretions1

Krabbenhoft¹,

Adams²,

Schipper³

1965

Applied Microbiology

View full text Add to dashboard Cite

, A. PAUL ADAMS, AND I. A. SCHIPPER. Antibiotic sensitivities of organisms isolated from mastitic and nonmastitic mammary secretions. Appl. Microbiol. 13:762-765. 1965.-Antibiotic sensitivity tests to determine the minimal inhibitory concentrations (MIC) and the minimal lethal concentrations (MLC) for six antibiotics were conducted against mastitic isolates of staphylococci in skim milk and broth. The MIC and the MLC for all the antibiotics except benzylpenicillin were considerably higher in skim milk than in broth. Benzylpenicillin was the most effective antibiotic tested in either medium, and dihydrostreptomycin was the least effective against the organisms tested.

show abstract

“…The stability of aureomycin in aqueous solution is strictly a function of pH and temperature (Pruess, 1954;Dornbuch and Peleak, 1948;Harned et al, 1948;Price et al, 1948). After passing through the alkaline medium of the small intestine of a chick at the body temperature of 39°C, the antibiotic would have been decomposed, especially when administered in such minute quantity as the dietary or nutritional dosage.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Aureomycin on the Thyroid Glands of Growing Chicks

1970

View full text Add to dashboard Cite

Bacteriological Studies of Aureomycin

Cited by 40 publications

References 2 publications

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINES^a,^b

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINES^a,^b

Antibiotic Sensitivities of Organisms Isolated from Mastitic and Nonmastitic Mammary Secretions1

The Effect of Aureomycin on the Thyroid Glands of Growing Chicks

Contact Info

Product

Resources

About

Bacteriological Studies of Aureomycin

Cited by 40 publications

References 2 publications

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINESa,b

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINESa,b

Antibiotic Sensitivities of Organisms Isolated from Mastitic and Nonmastitic Mammary Secretions1

The Effect of Aureomycin on the Thyroid Glands of Growing Chicks

Contact Info

Product

Resources

About

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINES^a,^b

ANTIBIOTIC RESIDUALS AND MICROBIAL RESISTANCE IN POULTRY TREATED WITH TETRACYCLINES^a,^b