Bacteriological quality of Nono, a milk product sold at retail outlets in Federal Capital Territory, Nigeria

Uzoaga, George Onyemauwa; Umeokonkwo, Chukwuma David; Usman, Aishat Bukola; Kia, G.S.N.; Okolocha, Emmanuel C.

doi:10.37432/jieph.2020.3.2.25

Cited by 5 publications

(10 citation statements)

References 10 publications

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“…For confirmation of both Escherichia coli and Staphylococcus aureus in the test samples, Gram staining and biochemical reactions including the Catalase and Coagulase tests were performed on wellisolated colonies from Eosin Methylene Blue (EMB) agar and Mannitol salt agar, respectively. These procedures were carried out as previously described (Uzoaga et al, 2020;Yakubu et al, 2020).…”

Section: Confirmation Of E Coli and S Aureusmentioning

confidence: 99%

“…Among the food of animal origins, raw milk, processed milk or its products represent good growth medium for pathogens, especially bacterial organisms, because of factors such as high water activity, relatively neutral pH as well a significant amount of nutrients (Abate and Wolde, 2015;Reta and Addis, 2015;Perin et al, 2018). These factors make milk an important culture medium for many bacterial pathogens; many of which are of public health importance (Uzoaga et al, 2020). When proper hygienic milking procedures and hygienic handling and storage are not applied along the steps of milk production at the farm level (milking and storage), transportation to dairy industries and processing, the result is increased contamination of milk with pathogenic organisms posing a public health hazard to humans (Perin et al, 2018;Rai et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…In Nigeria, there is a lot of concern regarding the process of milking, its transportation, storage, distribution and retailing in the milk value chain. This is due largely to the unhygienic practices at pre-and post-milking stages, the poor quality of water used as well as the utensils, and the lack of cold storage facilities and retailing environments (Uzoaga et al, 2020). Hawkers with poor knowledge of quality control and hygienic standards the retail majority of the milk or its products;…”

Section: Introductionmentioning

confidence: 99%

“…hence, subjecting milk to contamination with pathogenic bacteria of public health significance. Some studies have attempted to assess the microbiological quality of raw milk or its products in different parts of Nigeria ( Aliyu et al, 2019;Uzoaga et al, 2020;Yakubu et al, 2020). To the best of our knowledge, there is a paucity of information regarding this in Northeastern Nigeria, despite the region serving as the largest livestockproducing hub in the country.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence Of Escherichia Coli and Staphylococcus Aureus in Raw Milk and its Products Sold for Human Consumption in Maiduguri, Northeastern Nigeria: A Potential Public Health Concern

Wulgo.¹

2022

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Globally, milk represents an important human nutrition. However, it also serves as an important vehicle for transmission of foodborne pathogens some of which may be multidrug resistant. The current study was undertaken with the main aim of assessing the microbiological quality of raw milk and its products consumed in Maiduguri metropolis. Fresh milk was sampled from six different milk source outlets in the metropolis and the samples were subjected to standard microbiological procedures for the detection of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). A cross-sectional study was conducted with a total of 120 milk samples collected and cultured on appropriate growth and differential media and further biochemical tests were carried out in order to determine the presence of E. coli and S. aureus. A total of 66 (55.0%) of the 120 samples were contaminated with bacteria, while 54 (45.0%) milk samples tested were negative for the contaminants. Contaminating bacteria comprised 51 (42.5%) and 43 (35.8%) of S. aureus and E. coli respectively. The mean pH of the milk samples ranges from 3.3 to 6.3 respectively in ―Kindirmo‖ and fresh milk with an overall mean pH of 3.9. A high prevalence of both pathogens was detected in ―Kindirmo‖ and ―Nono‖ fermented milk products. Overall, a statistically significant association was found between the detection of Escherichia coli (p = 0.046) and Staphylococcus aureus (p = 0.013) with the different types of milk samples examined. Our findings of the high prevalence of these pathogens in readily consumed fresh milk and its products in the study area raise public health concerns. Therefore, we recommended the application of strict quality control and good hygienic standards along the milk value chain by application of hazard analysis and critical control points (HACCP). Public health awareness of the health risks associated with the consumption of contaminated milk or its products is also highly recommended.

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Section: Confirmation Of E Coli and S Aureusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence Of Escherichia Coli and Staphylococcus Aureus in Raw Milk and its Products Sold for Human Consumption in Maiduguri, Northeastern Nigeria: A Potential Public Health Concern

Wulgo.¹

2022

AJBAR

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“…Milk is a fluid secreted by the mammary gland of mammals for the nourishment of their young. Raw milk is composed of water, fats, proteins, carbohydrates, minerals and vitamins that favors growth of both harmless and pathogenic bacteria when suitable temperature exists (Uzoagu et al, 2020). This complex dairy product can be easily contaminated with enteric bacteria such as E. coli, Shigella, Salmonella and a host of other non-enteric bacteria which can reduced its quality and poses serious public health threat when ingested (Maikai & Madaki, 2018).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Antimicrobial Susceptibility Profile of Escherichia Coli Isolates from Vended Milk in University of Abuja Community, Federal Capital Territory, Nigeria.

Ameh¹,

Mailafia²,

Olabode³

et al. 2022

AJFSN

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Purpose: This study was conducted to establish occurrence and antimicrobial susceptibility profile of Escherichia coli (E. coli) isolates from cow milk (Nono) sold within University of Abuja campuses. Methodology: Fifty (50) samples of “nono” milk were randomly collected from vendors in mini and main campus of University of Abuja from August to October 2021. All samples were analyzed using pour and surface plating methods. Presumptive E. coli isolates on Eosin methylene blue agar were characterized using preliminary and standard biochemical identification methods such as microbial plate count, Gram staining, oxidase, indole, methyl red, voges-proskauer, citrate and catalase tests. Antimicrobial susceptibility testing was conducted on positive isolates using the disc diffusion method. Findings: Out of the 50 samples of nono milk cultured, thirty (30) yielded growth for Escherichia coli which appeared as greenish metallic sheen on EMB agar plate and Gram-negative short rods on microscopic examination, representing an overall prevalence of 60%. The overall viable count of all samples was extremely high, with 8.7x10^7cfu/ml recorded as the highest colony forming unit per milliliter (CFU/ml) and 1.5×10^7cfu/ml recorded the lowest colony forming unit per milliliter (CFU/ml). All isolates were indole positive, methyl red positive, oxidase negative, citrate negative, catalase positive and voges-proskauer negative. E. coli isolates in this study were susceptible to sparfloxacin (66.7%), gentamicin (66.7%), ciprofloxacin (60%), streptomycin (60%), and tarivid (53.3%) and resistant to Septrin (73.3%), Augumentin (60%), Pefloxacin (66.7%), chloramphenicol (60%) and amoxicillin (53.3%). In conclusion, this study showed that the degree of contamination of E. coli in vended milk in the study area is higher than the maximum permissible bacteria count recommended by Codex Alimentarius standard for fermented milk products which is indicative of potential hazard to consumers. Recommendation: Public enlightenment on hygienic handling of milk during milking and especially vending by milk vendors is hereby recommended. Use of cool thermos for milk hawking is also advocated.

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Prevalence of Escherichia coli O157 isolated from marketed raw cow milk in Kwara State, Nigeria

et al. 2023

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Bacteriological quality of Nono, a milk product sold at retail outlets in Federal Capital Territory, Nigeria

Cited by 5 publications

References 10 publications

Prevalence Of Escherichia Coli and Staphylococcus Aureus in Raw Milk and its Products Sold for Human Consumption in Maiduguri, Northeastern Nigeria: A Potential Public Health Concern

Prevalence Of Escherichia Coli and Staphylococcus Aureus in Raw Milk and its Products Sold for Human Consumption in Maiduguri, Northeastern Nigeria: A Potential Public Health Concern

Occurrence and Antimicrobial Susceptibility Profile of Escherichia Coli Isolates from Vended Milk in University of Abuja Community, Federal Capital Territory, Nigeria.

Prevalence of Escherichia coli O157 isolated from marketed raw cow milk in Kwara State, Nigeria

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