Bacteriological profile of urinary tract infections and antibiotic susceptibility of Escherichia coli in Algeria

Ait-Mimoune, Nouara; Hassaine, Houda; Boulanoir, Meriem

doi:10.18502/ijm.v14i2.9180

Cited by 12 publications

(6 citation statements)

References 16 publications

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“…In this study, the prevalence of uropathogens accounted for 68(34.0%) among pregnant women. This observation were in tandem with the earlier studies where a prevalence rate of 37.3%, 38.6%, 35.5%, 38% and 40.0% were reported in India, Lagos, Algeria, Niger Delta and Jos [16,17,18,19] but disagree with earlier report which accounted for 45.7, 53.8, 65.4%, 79.9%, 90.1% and 97.3% [20,21,22,23,24]. The different in prevalence rates reported may be due to factors like number of sample and duration of study, deployment of diverse methodologies, sexual intercourse, studied group influence, low socio-economic status among Nigerian Women.…”

Section: Discussionsupporting

confidence: 90%

Evaluating the Bacteria Profile and Drug Susceptibility Patterns of Urinary Tract Infectious Pathogens in Pregnant Women in Abakaliki Metropolis, Nigeria

Ekuma,

Ibiam,

Ekuma

et al. 2023

IJPR

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Patient’s demographic data were obtained from a well-structured questionnaire administered before sample collection. A total of two hundred (200) early morning mid-stream urine samples were collected from pregnant women attending Mile 4 hospital to determine the prevalence of UTIs among pregnant women. The collected samples were analysis using Standard Microbiology protocol for isolation and identification of uropathogens. Antibiogram studies of uropathogens were determined using the Kirby–Bauer disk diffusion method and the results were analyzed and were compared with the Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints. Results of isolation and characterization revealed an overall occurrence rate of 68(34.0%) bacteria consisting of a high distribution of E. coli 27(13.5%) followed by S. aureus 22 (11.0%) and Klebsiella pneumoniae 19 (9.5%). Socio-demographic data of patients revealed that those aged 42-49 years had a high frequency of bacteria 7(70.0%) followed by those aged 34-41 years 50.0%, 18-25 years 31.1% while those aged 26-33 year had the least isolation rate of 26.3%. The occurrence of UTI was highly predominant among patients with no formal level of Education 24(64.9%) than those with formal level of education 17(26.9%). The result of antibiogram studies shows that the isolates exhibited a high percentage of resistance to colistin 100%, azetronam 100% clindamycin 100%, and tetracycline 77.3% but were 92.6%-100% susceptible to cefoxitin, imipenem and amikacin. However, with substantial evidence in this study, cefoxitin, imipenem, and amikacin as drugs of choice could be used for the treatment of UTI, and further studies should be conducted by using highly sensitive and specific techniques such as Polymerase Chain Reaction (PCR), which is the technique used to make numerous copies of a specific segment of the deoxyribonucleic acid quickly and accurately, including genotypic characterization of resistant determinant in bacteria causing UTI among pregnant patients in a larger sample size.

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Section: Discussionsupporting

confidence: 90%

Evaluating the Bacteria Profile and Drug Susceptibility Patterns of Urinary Tract Infectious Pathogens in Pregnant Women in Abakaliki Metropolis, Nigeria

Ekuma,

Ibiam,

Ekuma

et al. 2023

IJPR

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“…This involved low susceptibility to aminopenicillins, third-generation cephalosporins and sulphonamides, medium susceptibility to quinolones and high susceptibility to aminoglycosides, especially amikacin, carbapenems and nitrofurantoin. 24 , 25 However, the proportions observed from our data were consistently lower. Lower susceptibility of the amoxicillin/clavulanic acid combination may have been influenced by batch-to-batch variations and storage conditions.…”

Section: Discussioncontrasting

confidence: 43%

Antimicrobial susceptibility testing data analysis over 3 years at the Yaoundé General Hospital, Cameroon

Ngogang,

Nkoth,

Ngaleu

et al. 2024

JAC-Antimicrobial Resistance

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Background Antimicrobial resistance (AMR) is a major health concern with high rates in low-income countries. Bacteriology laboratories sustain the fight against AMR by providing antibiotic susceptibility testing (AST) results to ensure appropriate therapies. These laboratories generate a lot of data, which are usually used for prospective interventions. Our study conducted in a lower-middle-income hospital setting aimed to describe the profile of bacteria isolated from the specimens received over 3 years, assess their susceptibility profile and identify potential gaps or area of improvement from the analysis of our data. Methods Monthly data were retrieved from registers for all specimens received between January 2020 until December 2022. Data were compiled and analysed using the R and WHONET software. Results Out of 3582 specimens received, 797 were culture positive (22.3%). Escherichia coli and Klebsiella pneumoniae were frequently isolated (30.5% and 24.2%, respectively). AST results analysis showed high resistance of Gram-negative bacteria to penams and cephems, whereas low resistance was observed to carbapenems. Susceptibility to antibiotics based on the AWaRe antibiotic classification was variable. The bacteriological profile in the various types of specimen was established and rational information to design a therapeutic protocol adapted to our hospital setting was obtained. Conclusions AST results may not only be used for prospective guidance for treatment, but rather cumulative data analysis can contribute to design effective antibiotic prescriptions and improve general practices at the laboratory. This is, however, dependent on a good record-keeping, standardization of practices and collaboration between clinicians and laboratory scientists.

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“…Uropathogenic E. coli (UPEC) is commonly associated with CAUTIs and comprises ∼80% of urinary tract infections. , WG-1 silicone achieved a log 5 (99.999%) reduction via a 24 h ASTM E2149 shake flask test. Separately, after WG-1 was immersed for 28 d in a pH 6.5 M3 medium, an ASTM E2149 shake flask test for the removed samples confirmed antimicrobial effectiveness against UPEC.…”

Section: Resultsmentioning

confidence: 99%

Facile Modification of Medical-Grade Silicone for Antimicrobial Effectiveness and Biocompatibility: A Potential Therapeutic Strategy against Bacterial Biofilms

Wynne,

Zolotarskaya,

Jarrell

et al. 2023

ACS Appl. Mater. Interfaces

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A one-step modification of biomedical silicone tubing with N,N-dimethyltetradecylamine, C14, results in a composition designated WinGard-1 (WG-1, 1.1 wt % C14). A surface-active silicon-amine phase (SAP) is proposed to account for increased wettability and increased surface charge. To understand the mechanism of antimicrobial effectiveness, several procedures were employed to detect whether C14 leaching occurred. An immersion-growth (IG) test was developed that required knowing the bacterial Minimum Inhibitory Concentrations (MICs) and Minimum Biocidal Concentrations (MBCs). The C14 MIC and MBC for Gm– uropathogenic E. coli (UPEC), commonly associated with catheter-associated urinary tract infections (CAUTI), were 10 and 20 μg/mL, respectively. After prior immersion of WG-1 silicone segments in a growth medium from 1 to 28 d, the IG test for the medium showed normal growth for UPEC over 24 h, indicating that the concentration of C14 must be less than the MIC, 10 μg/mL. GC-MS and studies of the medium inside and outside a dialysis bag containing WG-1 silicone segments supported de minimis leaching. Consequently, a 5 log UPEC reduction (99.999% kill) in 24 h using the shake flask test (ASTM E2149) cannot be due to leaching and is ascribed to contact kill. Interestingly, although the MBC was greater than 100 μg/mL for Pseudomonas aeruginosa, WG-1 silicone affected an 80% reduction via a 24 h shake flask test. For other bacteria and Candida albicans , greater than 99.9% shake flask kill may be understood by proposing increased wettability and concentration of charge illustrated in the TOC. De minimis leaching places WG-1 silicone at an advantage over conventional anti-infectives that rely on leaching of an antibiotic or heavy metals such as silver. The facile process for preparation of WG-1 silicone combined with biocidal effectiveness comprises progress toward the goals of device designation from the FDA for WG-1 and clearance.

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Bacteriological profile of urinary tract infections and antibiotic susceptibility of Escherichia coli in Algeria

Cited by 12 publications

References 16 publications

Evaluating the Bacteria Profile and Drug Susceptibility Patterns of Urinary Tract Infectious Pathogens in Pregnant Women in Abakaliki Metropolis, Nigeria

Evaluating the Bacteria Profile and Drug Susceptibility Patterns of Urinary Tract Infectious Pathogens in Pregnant Women in Abakaliki Metropolis, Nigeria

Antimicrobial susceptibility testing data analysis over 3 years at the Yaoundé General Hospital, Cameroon

Facile Modification of Medical-Grade Silicone for Antimicrobial Effectiveness and Biocompatibility: A Potential Therapeutic Strategy against Bacterial Biofilms

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