Bacteriological profile of selected fish species and water sample from Otuocha River Anambra State

Amuneke, K.E.; Igbodiegwu, G.C.; Okeke, P. A.; Adibe, A.C.

doi:10.4314/jafs.v18i1.2

Cited by 7 publications

(5 citation statements)

References 13 publications

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“…Fishes are affected by the bacterial load and the bacterial species that are present in the ecosystem; also, Ali et al [ 34 ] showed that fishes collected from polluted sites (drainage) recorded higher bacterial loads (in different fish organs) than fishes collected from nonpolluted sites. The results supported Amuneke et al [ 35 ] and Khairy et al [ 36 ]. On the other hand, the current research findings were higher than those provided by Mamdouh et al [ 37 ], who reported 4.83 ± 0.43 log cfu/g in O. niloticus and lower than the finding of Ali [ 38 ], who reported TAC of 6.36 to 7.89 log cfu/g for fishes in Abu Simbel Khor.…”

Section: Discussionsupporting

confidence: 89%

Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt

Elbarbary,

Dandrawy,

Hadad

et al. 2024

International Journal of Food Science

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The microbial analysis of fish is critical for ensuring overall health. Uncooked fish can serve as a conduit for transmitting several types of microbes; the current investigation sought to assess the bacterial levels in various kinds of fish from Nasser Lake, Aswan, Egypt, considered the chief source of potable water in Egypt. Two hundred and fifty fish samples, including 50 of each Oreochromis niloticus, Sander lucioperca, Lates niloticus, Clarias gariepinus, and Mormyrus kannume, from Nasser Lake, Aswan, Egypt, were collected to detect the bacterial load, isolation, and identification of Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio parahaemolyticus and their virulence genes. The findings revealed that Oreochromis niloticus and Clarias gariepinus exhibited higher bacterial loads than other fish species. Incidences of bacterial contamination among examined fishes were 28.8%, 20.4%, and 16% for Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio parahaemolyticus, respectively. Additionally, PCR analysis detected the presence of aerA (60%) and Act (40%) genes in A. hydrophila, rpoB (70%) and LasB (30%) genes in P. aeruginosa, and ToxR (70%) and tdh (50%) genes in V. parahaemolyticus. The study suggested that the bacterial contamination levels in Oreochromis niloticus and Clarias gariepinus could be notably more significant than in other species that could potentially be harmful to the consumers, especially considering the identification of particular bacteria known to cause foodborne illnesses. Further recommendations emphasized that regular monitoring and assessments are required to preserve their quality.

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Section: Discussionsupporting

confidence: 89%

Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt

Elbarbary,

Dandrawy,

Hadad

et al. 2024

International Journal of Food Science

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“…coli distribution from fishpond water revealed an overall occurrence rate of 97 (32.3 %). The major findings of this study reiterate with report in Northern Ethiopia [23], Nigeria [24], Bangladesh [25], Northwest of Borneo [26] and Malaysia [27] where these bacteria were isolated in fishpond.…”

Section: Discussionsupporting

confidence: 86%

Distribution and Antibiotic Resistance Profile of Extended Spectrum Beta-Lactamase Producing Escherichia coli from Fish Farms within Abakaliki Metropolis

Adibe-Nwafor,

Uduku,

Iroha

et al. 2023

AIR

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Background and Objectives: The emergence of antibiotic resistant determinant in fish farms and its spread is on the increase and has evolved into strains that are resistant to many classes of antibiotics. Thus, it is critical to identify the distribution and antibiotic resistance profile of Extended Spectrum Beta-lactamase (ESBL) producing Escherichia coli from fish farms within Abakaliki Metropolis. Methodology: Aseptically, fifty (50) milliliters of fishpond water was collected from twenty locations in fifteen (15) fish farms and were analyze using standard microbiological culture and identification of Escherichia coli. Detection of phenotypic extended spectrum β-lactamases production was performed using Double-Disk Synergy Test (DDST). Antibiotic susceptibility studies of extended spectrum β-lactamases producing Escherichia coli was determined using the Kirby–Bauer disk diffusion method and the results were construed using the Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints. Results: Extended spectrum beta-lactamase producing Escherichia coli distribution from fishpond water revealed overall occurrence rate of 34(11.3 %). The proportion of ESBL producing Escherichia coli was 5(25.0 %) from fish farm L followed by Farm A, Farm E, Farm G, which both accounted for 20.0 % respectively while the least occurrence of 1(5.0 %) was recorded against Farm I. ESBL-producing E. coli were resistant to cephalosporin particularly Ceftriaxone (88.2%), Ceftazidime (91.2 %), Cefotaxime (94.1) and Cefepime (85.3 %). This was followed by Amoxicillin-Clavulanate (91. 2 %), Azetronam (97.1 %). In all, Ciprofloxacin (82.4 %), Imipenem (97.1 %), and Meropenem (100 %) were the most effective antibiotic against ESBL-producing E. coli isolate. Conclusion: This study reveals the prevalence of the ESBL phenotype in fish farms. The increasing prevalence of resistance to routinely used antibiotics in medical and veterinary therapies among the study isolates from aquaculture products poses a significant challenge to the treatment of human and animal diseases. As a result, adequate antibiotic intervention is essential to ensure the continued efficacy of antibiotics for aquaculture and human health, as well as the industry's viability.

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“…In Nigeria, fish is recognized and cherished as a healthy, safe and a cheap source of protein (Amuneke et al, 2020) adding an average of 17% to the protein intake (Boyd et al, 2022), and per capita consumption of 20.2 kg per annum in 2020, and a total consumption of 157 million Mt (FAO, 2022). Fish importation as reported by the Minister of Agriculture and Rural Development accounts for about 75% of the total consumption.…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin, bacterial and heavy metal load in Scomber scombrus and Clupea harengus from two selected coldroom facilities in Kwara State, Nigeria

Oghenochuko,

Kolawole,

Olajide

et al. 2023

EgeJFAS

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Impurities found in fish have been a major cause of disease and illness to consumers. This study's objective was to evaluate the total aflatoxin, heavy metal and microbial load in two frozen fish: Scomber scombrus and Clupea harengus from two (2) cold room facilities in Omu-Aran and Ilorin. Heavy metals, aflatoxin and microbial load were investigated using standard methods. Health risk was also determined using health risk index (HRI), daily intake of metals (DIM), and health quotient (HQ), and total toxicity of metals (TTM). Cd, Cu, Ni, Pb, Mn and Cr did not differ significantly (p>0.05) in both species from both locations. Nickel was lower than the recommended limit by World Health Organization (WHO). Level of Mn and Cr were higher in both species. Mn load was higher in the muscles of the sampled fish than in the gills from September through to November with highest value of 1.26±0.08 and 1.30±0.12 obtained for S. scombrus and C. harengus. Highest concentrations of all metals was observed in the gills except manganese and copper [Cd = 0.03 (S. scombrus), Cr = 1.22±0.13 (S. scombrus), Ni = 0.025±0.04 (S. scombrus), Pb = 0.06±0.02 (S. scombrus)]. HRI was > 1 in the different age groups for the different metals. TTM was > 1 in both species. Total aflatoxin level was higher in the gills (4.25 – 5) ppb than in the muscle (1.5 - 3) ppb for both locations respectively. Vibrio spp. and non-coliform bacteria were high in both species from both locations. The study concludes that heavy metal loads (Mn, Cd, Cu, Cr, and Ni) were more than the permitted limitations imposed by FAO, WHO and EU legislation for fish and fish products placing consumers at health risk.

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Bacteriological profile of selected fish species and water sample from Otuocha River Anambra State

Cited by 7 publications

References 13 publications

Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt

Bacterial Quality and Molecular Detection of Food Poisoning Virulence Genes Isolated from Nasser Lake Fish, Aswan, Egypt

Distribution and Antibiotic Resistance Profile of Extended Spectrum Beta-Lactamase Producing Escherichia coli from Fish Farms within Abakaliki Metropolis

Aflatoxin, bacterial and heavy metal load in Scomber scombrus and Clupea harengus from two selected coldroom facilities in Kwara State, Nigeria

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