Bacteriological Profile of Chronic Suppurative Otitis Media and Antibiotic Susceptibility in a Tertiary Care Hospital in Shanghai, China

Xu, Jianghong; Du, Qiang; Shu, Yilai; Ji, Jian; Dai, Chunfu

doi:10.1177/0145561320923823

Cited by 21 publications

(22 citation statements)

References 21 publications

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“…Incidence of otitis media is lower in adults compared to childhood [12], for example is the percentage shown for children under the age of 6 in Nigeria which is 9.2% [13]. Similar to CSMO in Colombia [14] and China [15], the majority of CSOM cases are on people over 20 years old. This result can occur if the patient had a discharge from the ear (otorrhea) since childhood and the symptoms persist into adulthood.…”

Section: Discussionmentioning

confidence: 92%

Chronic Suppurative Otitis Media Characteristic in Secondary Hospital in Yogyakarta

Suryani

Widuri

2022

Open Access Maced J Med Sci

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BACKGROUND: Chronic suppurative otitis media (CSOM) is the most common ear infection that causes hearing loss and affects the quality of life worldwide. AIM: The study aimed to describe the risk factor of CSOM patients at a private hospital in Yogyakarta, Indonesia, from March to June 2021. METHODS: This study was an observational analytical study with a case–control design. The respondents were 55 patients with CSOM who met inclusion criteria and 56 respondents as controls. A checklist interview was conducted to determine the present and past history of diseases, and the relationship among potential risk factors was analyzed using bivariate analysis. A bivariate analysis was used to specify the odds ratios, 95% confidence intervals, and the relationship between risk factors and the incidence of CSMO. RESULTS: The characteristic cases were as follows: Majority age over 20 years, 56% male, 47% university degree, 29% history of allergy, 25% history of acute respiratory infection, 56% complain of discharge from the ear, 31% snoring, 67% cigarette consumption, 42% earn 2–5 million rupiahs per month, and 73% of their homes are closed to health-care facilities. Four risk factors for CSOM omit this included: Education, medical history from the ear, snoring, and income per month (p < 0.05). CONCLUSION: According to the results, the majority of CSOM patients to be treated at a private clinic in Yogyakarta were male at productive age, had university education, had no history of allergies and acute respiratory infections, and had a history of secrete from the ear, exposure to cigarettes, and median income per month.

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Section: Discussionmentioning

confidence: 92%

Chronic Suppurative Otitis Media Characteristic in Secondary Hospital in Yogyakarta

Suryani

Widuri

2022

Open Access Maced J Med Sci

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“…This situation is common among populations with limited access to otologic health care, particularly in lower-income countries or marginalized groups. Taken together, the differences that we observed across age quartiles are likely to reflect [1] long-standing, untreated or inadequately treated OM, and [2] recent antibiotic use on top of a potentially long history of antibiotic prescription (local or oral) and high likelihood of antibiotic resistance ( Xu et al., 2020 ).…”

Section: Discussionmentioning

confidence: 98%

“…On the other hand, the most abundant taxa in cholesteatoma tissues were Corynebacterium , Porphyromonas , and Staphylococcus , with 11.5%, 8.3%, and 8.0% mean abundances ( Table S1 , Figure 1 ). Staphylococcus is often isolated from COM samples, but may be resistant to penicillin, macrolides or quinolones ( Xu et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiota Associated With Cholesteatoma Tissue in Chronic Suppurative Otitis Media

Frank

Magno

Velasco

et al. 2022

Front. Cell. Infect. Microbiol.

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Otitis media (OM), defined as infection or inflammation of the middle ear (ME), remains a major public health problem worldwide. Cholesteatoma is a non-cancerous, cyst-like lesion in the ME that may be acquired due to chronic OM and cause disabling complications. Surgery is required for treatment, with high rates of recurrence. Current antibiotic treatments have been largely targeted to previous culturable bacteria, which may lead to antibiotic resistance or treatment failures. For this study, our goal was to determine the microbiota of cholesteatoma tissue in comparison with other ME tissues in patients with long-standing chronic OM. ME samples including cholesteatoma, granulation tissue, ME mucosa and discharge were collected from patients undergoing tympanomastoidectomy surgery for chronic OM. Bacteria were profiled by 16S rRNA gene sequencing in 103 ME samples from 53 patients. Respiratory viruses were also screened in 115 specimens from 45 patients. Differences in bacterial profiles (beta-diversity) and the relative abundances of individual taxa were observed between cholesteatoma and ME sample-types. Additionally, patient age was associated with differences in overall microbiota composition while numerous individual taxa were differentially abundant across age quartiles. No viruses were identified in screened ME samples. Biodiversity was moderately lower in cholesteatoma and ME discharge compared to ME mucosal tissues. We also present overall bacterial profiles of ME tissues by sample-type, age, cholesteatoma diagnosis and quinolone use, including prevalent bacterial taxa. Our findings will be useful for fine-tuning treatment protocols for cholesteatoma and chronic OM in settings with limited health care resources.

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“…Moreover, in the majority of cases, species of the S. ciferrii complex are not identified. CSOM is prevalent in China just like most other developing countries and usually caused by bacteria ( Xu et al, 2020 ). CSOM caused by S. ciferrii complex is rarely reported.…”

Section: Discussionmentioning

confidence: 99%

“…Chronic suppurative otitis media (CSOM) is a chronic inflammation, characterized by repeated otorrhea via tympanic membrane perforation and involving polymicrobial infection of the middle ear and mastoid cavity ( Bhutta et al, 2020 ). Usually, the common pathogens causing CSOM are Staphylococcus aureus , Pseudomonas aeruginosa , and coagulase-negative Staphylococcus ( Xu et al, 2020 ). However, the isolation rate of S. ciferrii complex has increased recently in our hospital.…”

Section: Introductionmentioning

confidence: 99%

Identification and Antifungal Susceptibility Analysis of Stephanoascus ciferrii Complex Species Isolated From Patients With Chronic Suppurative Otitis Media

et al. 2021

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BackgroundStephanoascus ciferrii is a heterothallic ascomycetous yeast-like fungus. Recently, the concept of S. ciferrii complex has been proposed and it consists of S. ciferrii, Candida allociferrii, and Candida mucifera. We aimed to identify 32 strains of S. ciferrii complex isolated from patients with chronic suppurative otitis media (CSOM) at the species level and analyze the morphology and antifungal susceptibility profiles of the three species.MethodThe sequencing of the internal transcribed spacer (ITS) region and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify S. ciferrii complex species. The SARAMIS software was used for cluster analysis of the mass spectra. All the strains were cultured on Sabouraud dextrose agar (SDA) and CHROM plates for 7 days. In the meantime, colonies of the 32 strains went through Gram staining. The Sensititre YeastOne YO10 colorimetric panel was used for the antifungal susceptibility analysis.ResultsThere were 10 strains of C. allociferrii (31.25%), six strains of C. mucifera (18.75%), and 16 strains of S. ciferrii (50%) in the 32 strains of S. ciferrii complex according to the sequencing of the ITS region. MALDI-TOF MS could identify S. ciferrii but showed no results for C. allociferrii and C. mucifera. The cluster analysis of the mass spectra by SARAMIS indicated that the MALDI-TOF MS could distinguish the three species. The morphology characteristics of the three species were similar. As for antifungal susceptibility, S. ciferrii and C. mucifera tended to have high fluconazole MICs compared with C. allociferrii. C. mucifera and C. allociferrii had relatively low flucytosine MICs while S. ciferrii owned high flucytosine MICs. Besides, C. mucifera tended to have a higher MIC value than S. ciferrii for amphotericin B and C. allociferrii for anidulafungin, micafungin, and caspofungin.ConclusionThe antifungal susceptibility profiles of the three species of S. ciferrii complex had their own characteristics. Besides, more mass spectra of C. allociferrii and C. mucifera are needed to construct the reference database for S. ciferrii complex species, enabling MALDI-TOF MS to identify S. ciferrii complex at species level.

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Bacteriological Profile of Chronic Suppurative Otitis Media and Antibiotic Susceptibility in a Tertiary Care Hospital in Shanghai, China

Cited by 21 publications

References 21 publications

Chronic Suppurative Otitis Media Characteristic in Secondary Hospital in Yogyakarta

Chronic Suppurative Otitis Media Characteristic in Secondary Hospital in Yogyakarta

Microbiota Associated With Cholesteatoma Tissue in Chronic Suppurative Otitis Media

Identification and Antifungal Susceptibility Analysis of Stephanoascus ciferrii Complex Species Isolated From Patients With Chronic Suppurative Otitis Media

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