Bacteriological Profile of Blood Culture Positive Sepsis in Newborn at BPKIHS, Dharan Nepal

Kanodia, Piush; Yadav, Sunil Kumar; Singh, Rupa; Bhatta, Narendra

doi:10.3126/jcmsn.v13i1.16663

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…This was higher than from a report in Germany (18.6 %) [ 21 ]. However, our finding was still lower than from another study in Nepal which reported that blood culture positivity among newborns with sepsis was 32.4 % [ 22 ]. Meanwhile, a study in Pakistan reported that blood culture positivity among paediatric septicaemia patients was 36 % [ 23 ].…”

Section: Discussioncontrasting

confidence: 94%

“…A different finding was reported in Nepal, which showed that more than 50 % of organisms isolated from blood from patients in an NICU were S. aureus [22].…”

Section: Discussionmentioning

confidence: 78%

“…Our finding could be higher because we collected not only from the PICU but also from other wards, such as emergency and neonatal intensive care unit (NICU) wards. A different finding was reported in Nepal, which showed that more than 50 % of organisms isolated from blood from patients in an NICU were S. aureus [ 22 ].…”

Section: Discussionmentioning

confidence: 78%

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Application of a sepsis flow chip (SFC) assay for the molecular diagnosis of paediatric sepsis

Prasetyo

Karyanti

Yuniar

et al. 2023

Access Microbiology

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A delay in detecting sepsis pathogens is a problematic issue for determining definitive antibiotic therapy for the causative pathogens. The gold standard method for sepsis is blood culture but this requires 3 days to detect the definitive pathogen. Molecular methods offer rapid identification of pathogens. We evaluated the use of sepsis flow chip (SFC) assay for identifying pathogens from children with sepsis. Blood samples from children with sepsis were collected and incubated in a culture device. Positive samples were subjected to amplification-hybridization using SFC assay and culture. A total of 94 samples from 47 patients were recovered, from which 25 isolates were recovered, including Klebsiella pneumoniae (11) and Staphylococcus epidermidis (6). From 25 positive blood culture bottles subjected to SFC assay, 24 genus/species and 18 resistance genes were detected. The sensitivity, specificity and conformity was 80, 94.2 and 94.68 % respectively. SFC assay offers promise to identify pathogens from positive blood culture in paediatric patients with sepsis and may support the antimicrobial stewardship programme in hospitals.

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Section: Discussioncontrasting

confidence: 94%

“…A different finding was reported in Nepal, which showed that more than 50 % of organisms isolated from blood from patients in an NICU were S. aureus [22].…”

Section: Discussionmentioning

confidence: 78%

See 1 more Smart Citation

Application of a sepsis flow chip (SFC) assay for the molecular diagnosis of paediatric sepsis

Prasetyo

Karyanti

Yuniar

et al. 2023

Access Microbiology

View full text Add to dashboard Cite

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Gram-negative neonatal sepsis in low- and lower-middle-income countries and WHO empirical antibiotic recommendations: A systematic review and meta-analysis

et al. 2021

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Background Neonatal sepsis is a significant global health issue associated with marked regional disparities in mortality. Antimicrobial resistance (AMR) is a growing concern in Gram-negative organisms, which increasingly predominate in neonatal sepsis, and existing WHO empirical antibiotic recommendations may no longer be appropriate. Previous systematic reviews have been limited to specific low- and middle-income countries. We therefore completed a systematic review and meta-analysis of available data from all low- and lower-middle-income countries (LLMICs) since 2010, with a focus on regional differences in Gram-negative infections and AMR. Methods and findings All studies published from 1 January 2010 to 21 April 2021 about microbiologically confirmed bloodstream infections or meningitis in neonates and AMR in LLMICs were assessed for eligibility. Small case series, studies with a small number of Gram-negative isolates (<10), and studies with a majority of isolates prior to 2010 were excluded. Main outcomes were pooled proportions of Escherichia coli, Klebsiella, Enterobacter, Pseudomonas, Acinetobacter and AMR. We included 88 studies (4 cohort studies, 3 randomised controlled studies, and 81 cross-sectional studies) comprising 10,458 Gram-negative isolates from 19 LLMICs. No studies were identified outside of Africa and Asia. The estimated pooled proportion of neonatal sepsis caused by Gram-negative organisms was 60% (95% CI 55% to 65%). Klebsiella spp. was the most common, with a pooled proportion of 38% of Gram-negative sepsis (95% CI 33% to 43%). Regional differences were observed, with higher proportions of Acinetobacter spp. in Asia and Klebsiella spp. in Africa. Resistance to aminoglycosides and third-generation cephalosporins ranged from 42% to 69% and from 59% to 84%, respectively. Study limitations include significant heterogeneity among included studies, exclusion of upper-middle-income countries, and potential sampling bias, with the majority of studies from tertiary hospital settings, which may overestimate the burden caused by Gram-negative bacteria. Conclusions Gram-negative bacteria are an important cause of neonatal sepsis in LLMICs and are associated with significant rates of resistance to WHO-recommended first- and second-line empirical antibiotics. AMR surveillance should underpin region-specific empirical treatment recommendations. Meanwhile, a significant global commitment to accessible and effective antimicrobials for neonates is required.

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Gram-Negative Neonatal Sepsis in Low- and Middle- Income Countries: How Appropriate are the WHO Recommended Empirical Sepsis Antibiotic Regimens? A Systematic Review and Meta-Analysis

et al. 2021

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Bacteriological Profile of Blood Culture Positive Sepsis in Newborn at BPKIHS, Dharan Nepal

Cited by 4 publications

References 12 publications

Application of a sepsis flow chip (SFC) assay for the molecular diagnosis of paediatric sepsis

Application of a sepsis flow chip (SFC) assay for the molecular diagnosis of paediatric sepsis

Gram-negative neonatal sepsis in low- and lower-middle-income countries and WHO empirical antibiotic recommendations: A systematic review and meta-analysis

Gram-Negative Neonatal Sepsis in Low- and Middle- Income Countries: How Appropriate are the WHO Recommended Empirical Sepsis Antibiotic Regimens? A Systematic Review and Meta-Analysis

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