Abstract:The Group I paracolon bacilli formed biochemically a stable group. They fermented glucose, maltose, mannite, and dulcite, but not saccharose. Most of them could be distinguished from Salmonella organisms by the late fermentation of lactose or the production of indole. Many resembled the Newcastle bacillus in being late-dulcite fermenters. Serologically 75% of the strains were identical, each containing the A antigen. Some formed a separate group containing the A antigen, andBact. alkalescensantigen and the C a… Show more
“…Four of them did not produce gas in MacConkey's broth a t 37'. This agrees with the results obtained by Stuart, Griffin & Baker (1938), who st,reaked eosin methyleae blue agar plates with decayed parts of fruits and vegetables, and employed incubation a t room temperature for 48 hr. Twenty-four of their 42 cultures produced gas from lactose at room temperature but not a t 37", 5 produced acid alone a t either temperature and only 13 produced acid and gas a t 37".…”
Section: Discussionsupporting
confidence: 91%
“…a t 44' in a modified Eijkman medium. Mackenzie & Hilton-Sergeant (1938), Bardsley (1938Bardsley ( , 1948, Clegg & Sherwood (1939, 1947, Clegg (1941), Sherwood & Clegg (1942) and Ferramola (1940) have confirmed that Bact. coli type I almost invariably forms gas from lactose in MacConkey's broth a t 44".…”
Section: S B Thomas G Elis Jones and Patricia M Franklinmentioning
confidence: 91%
“…According to Stuart, Mickle & Borman (1940) some anaerogenic cultures do not produce gas from any carbohydrate and would ordinarily be classed as Shigella or 8almonella. The relationship between anaerogenic cultures and other members of the coliaerogenes group is not entirely clear, Stuart, Griffin & Baker (1938) found that many typical coli-aerogenes cultures isolated from soil, water or milk may lose their ability to produce gas in lactose, while Stuart, Mickle & Rorman (1940) were inclined to believe that anaerogenic cultures isolated from soil, water or cereals are for the most part more primitive coliforms whereas those obtained from apparently normal or pathological faeces may merely be altered normal coliforms, non-pathogenic themselves but suggestive of a pathological condition.…”
Section: S B Thomas G Elis Jones and Patricia M Franklinmentioning
Summary:
The methods suggested in the Report of the Coliform Sub‐Committee of the Society for Applied Bacteriology (Report, 1949) have been used in isolating and studying the coli‐aerogenes content of 142 samples of farm water supplies in two distinctive areas of Britain. The Gram‐negative cultures were grouped into typical, anaerogenic, non‐lactose‐fermenting and ‘unclassifiable’types, forming 54.2, 2.9, 10.9 and 32.0% of the 384 cultures respectively. Among the 261 coli‐aerogenes cultures, Bact. coli, intermediate and aerogenes‐cloacae organisms were found with frequencies of 32.2, 19.5 and 48.3% respectively. The majority of anaerogenic coli‐aerogenes organisms resembled Bact. coli type II while the predominant non‐lactose‐fermenters resembled Bact. cloacae and Bact. aerogenes type I. The reactions of 123 ‘unclassifiable'strains were such that very few could be regarded as Bact. coli types owing to the high incidence of gelatin liquefaction and growth in citrate broth.
“…Four of them did not produce gas in MacConkey's broth a t 37'. This agrees with the results obtained by Stuart, Griffin & Baker (1938), who st,reaked eosin methyleae blue agar plates with decayed parts of fruits and vegetables, and employed incubation a t room temperature for 48 hr. Twenty-four of their 42 cultures produced gas from lactose at room temperature but not a t 37", 5 produced acid alone a t either temperature and only 13 produced acid and gas a t 37".…”
Section: Discussionsupporting
confidence: 91%
“…a t 44' in a modified Eijkman medium. Mackenzie & Hilton-Sergeant (1938), Bardsley (1938Bardsley ( , 1948, Clegg & Sherwood (1939, 1947, Clegg (1941), Sherwood & Clegg (1942) and Ferramola (1940) have confirmed that Bact. coli type I almost invariably forms gas from lactose in MacConkey's broth a t 44".…”
Section: S B Thomas G Elis Jones and Patricia M Franklinmentioning
confidence: 91%
“…According to Stuart, Mickle & Borman (1940) some anaerogenic cultures do not produce gas from any carbohydrate and would ordinarily be classed as Shigella or 8almonella. The relationship between anaerogenic cultures and other members of the coliaerogenes group is not entirely clear, Stuart, Griffin & Baker (1938) found that many typical coli-aerogenes cultures isolated from soil, water or milk may lose their ability to produce gas in lactose, while Stuart, Mickle & Rorman (1940) were inclined to believe that anaerogenic cultures isolated from soil, water or cereals are for the most part more primitive coliforms whereas those obtained from apparently normal or pathological faeces may merely be altered normal coliforms, non-pathogenic themselves but suggestive of a pathological condition.…”
Section: S B Thomas G Elis Jones and Patricia M Franklinmentioning
Summary:
The methods suggested in the Report of the Coliform Sub‐Committee of the Society for Applied Bacteriology (Report, 1949) have been used in isolating and studying the coli‐aerogenes content of 142 samples of farm water supplies in two distinctive areas of Britain. The Gram‐negative cultures were grouped into typical, anaerogenic, non‐lactose‐fermenting and ‘unclassifiable’types, forming 54.2, 2.9, 10.9 and 32.0% of the 384 cultures respectively. Among the 261 coli‐aerogenes cultures, Bact. coli, intermediate and aerogenes‐cloacae organisms were found with frequencies of 32.2, 19.5 and 48.3% respectively. The majority of anaerogenic coli‐aerogenes organisms resembled Bact. coli type II while the predominant non‐lactose‐fermenters resembled Bact. cloacae and Bact. aerogenes type I. The reactions of 123 ‘unclassifiable'strains were such that very few could be regarded as Bact. coli types owing to the high incidence of gelatin liquefaction and growth in citrate broth.
IT is often assumed that the serum of a healthy uninoculated rabbit contains only insignificant amounts of antibacterial agglutinins. This view is largely supported by the findings of Gibson (1930), but Boyd (1939-40) and Messer (1943) have drawn attention to the occurrence of agglutinins in considerable concentration in some " normal " rabbit sera. Indeed, with a agglutinin, the natural antibody titres may be as high as those found in sera from immunised animals (Stamp and Stone, 1943-44 ; Francis and Buckland, 1945).I n the present work, certain paracolon bacilli isolated from desoxycholate-citrate-agar plate cultures during the examination of fieces from cases of enteritis or their contacts were found to have biochemical reactions superficially resembling those of certain specific pathogens and to be agglutinated to titre by a considerable number of batches of Oxford standard agglutinating sera prepared against different organisms. I n preliminary absorption experiments with antisera to Shigella sonnei and Shigella jlexneri, types Newcastle and Boyd 103, the paracolon bacilli failed to remove the specific agglutinins for the dysentery bacilli, and the homologous dysentery bacilli had no effect on the ability of the sera to agglutinate the paracolon strains. Subsequently the paracolon strains were found to be agglutinated to high titres by the sera of two healthy uninoculated rabbits. These rabbit sera also agglutinated lactose-fermenting and non-lactosefermenting coliforms from human fieces or contaminated war wounds. The agglutinins in these sera were studied in absorption experiments and the results form the subject of this paper.
MethodsStrains. Four strains of paracolon bacilli (PR, PW and PM from faxes, P 6 from a contaminated war wound) and 4 strains of Bacterium coli of Wilson et al. (1935), type I (CA and CB from fseces, C 5 and C 7 from wounds) were used for the main absorption experiments. Part of the work was repeated with three known tc strains, including the classical 1721, kindly provided by Dr Doris Stone.Bacterial suspensions. The organisms grown on Lemco-agar slopes for 18 hours at 37' C. were washed off in saline (0.85 per cent. sodium chloride)
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