Bacterioides fragilis: Production and Sensitivity to Bacteriocins

Papastathopoulou, A.; Bezirtzoglou, Eugenia; Legakis, N. J.

doi:10.1006/anae.1997.0106

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Numerous Bacteroides clinical isolates displayed bacteriocin production and sensitivity. 45, 46 These diverse responses point to the complexity of microbial niche establishment, also laying the groundwork for probiotic engineering that may exploit advantageous fitness factors. Similar to an oral bacterial strain replacement therapy devised to prevent dental cavities caused by acidic Streptococcus , 47 specific intestinal isolates, such as 9343, could be engineered to outcompete and replace pathogenic gut bacteria, possibly through potent bacteriocin production.…”

Section: Discussionmentioning

confidence: 99%

Non-toxigenic Bacteroides fragilis (NTBF) administration reduces bacteria-driven chronic colitis and tumor development independent of polysaccharide A

Chan

Geis

et al. 2019

Mucosal Immunology

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Polysaccharide A (PSA), an immunogenic capsular component of non-toxigenic Bacteroides fragilis (NTBF) strain NCTC 9343, is reported to promote mucosal immune development and suppress colitis. Contrastingly, enterotoxigenic Bacteroides fragilis (ETBF) is highly associated with inflammatory bowel disease (IBD) and colorectal cancer (CRC), rapidly inducing IL-17-dependent murine colitis and tumorigenesis. In specific-pathogen-free (SPF) C57BL/6 wild type (WT) and multiple intestinal neoplasia (Min Apc 716+/− ) mice, we show that sequential treatment of the NTBF strain, 9343, followed by the ETBF strain, 86–5443-2–2 (86), diminished colitis and tumorigenesis. Mice treated simultaneously with 9343 and 86 exhibited both severe colitis and tumorigenesis. Abrogated disease severity in sequentially treated mice was attributed to 9343 strain dominance and decreased IL-17A, but 86 colonization prior to or simultaneous with 9343 mitigated the anti-inflammatory effect of 9343. Remarkably, 9343-mediated protection was independent of PSA, as sequentially treated mice receiving ΔPSA 9343 exhibited similar protection. Further, SPF WT and Min mice colonized with PSA-competent or PSA-deficient 9343 exhibited similar IL-10, IL-17, and IFN-γ responses. Treatment of 86-colonized mice with 9343 failed to disrupt 86 pathogenesis. Our findings demonstrate 9343 colonization, independent of PSA, offers prophylaxis against colitis-inducing 86 but may not be a valid therapy once colitis is established.

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Section: Discussionmentioning

confidence: 99%

Non-toxigenic Bacteroides fragilis (NTBF) administration reduces bacteria-driven chronic colitis and tumor development independent of polysaccharide A

Chan

Geis

et al. 2019

Mucosal Immunology

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“…Production of antimicrobial proteins by intestinal Bacteroides species has been reported, with the most recent study in 1999 (Miranda et al ., ; Farias et al ., ; Papastathopoulou et al ., ; Avelar et al ., ). However, no responsible gene was cloned nor was the protein purified, therefore, the identity of these molecules remains unknown.…”

Section: Introductionmentioning

confidence: 98%

An antimicrobial protein of the gut symbiont Bacteroides fragilis with a MACPF domain of host immune proteins

Chatzidaki‐Livanis

Coyne

Comstock

2014

Molecular Microbiology

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Summary Bacteroidales are the most abundant Gram negative bacteria of the human intestinal microbiota comprising more than half of the bacteria in many individuals. Some of the factors that these bacteria use to establish and maintain themselves in this ecosystem are beginning to be identified. However, ecological competition, especially interference competition where one organism directly harms another, is largely unexplored. To begin to understand the relevance of this ecological principle as it applies to these abundant gut bacteria and factors that may promote such competition, we screened Bacteroides fragilis for the production of antimicrobial molecules. We found that the production of extracellularly secreted antimicrobial molecules is widespread in this species. The first identified molecule, described in this manuscript, contains a membrane attack complex/perforin (MACPF) domain present in host immune molecules that kill bacteria and virally infected cells by pore formation, and mutations affecting key residues of this domain abrogated its activity. This antimicrobial molecule, termed BSAP-1, is secreted from the cell in outer membrane vesicles and no additional proteins are required for its secretion, processing or immunity of the producing cell. This study provides the first insight into secreted molecules that promote competitive interference among Bacteroidales strains of the human gut.

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“…While limited information about B. fragilis OM proteins has emerged from bioinfomatic and experimental studies, the nature and identity of proteins that are secreted into the extracellular space is completely unknown. Available evidence indicates that B. fragilis secretes bacteriocins [ 31 , 32 ], but polypeptides that mediate antimicrobial activities have not been purified. Furthermore, it is possible that B. fragilis secretes proteases, glycosidases and hemolysins that facilitate nutrient and iron uptake as well as proteins that modulate the activity of host cells.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Outer Membrane Proteome and Secretome of Bacteroides fragilis Reveals a Multiplicity of Secretion Mechanisms

2015

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Bacteroides fragilis is a widely distributed member of the human gut microbiome and an opportunistic pathogen. Cell surface molecules produced by this organism likely play important roles in colonization, communication with other microbes, and pathogenicity, but the protein composition of the outer membrane (OM) and the mechanisms used to transport polypeptides into the extracellular space are poorly characterized. Here we used LC-MS/MS to analyze the OM proteome and secretome of B. fragilis NCTC 9343 grown under laboratory conditions. Of the 229 OM proteins that we identified, 108 are predicted to be lipoproteins, and 61 are predicted to be TonB-dependent transporters. Based on their proximity to genes encoding TonB-dependent transporters, many of the lipoprotein genes likely encode proteins involved in nutrient or small molecule uptake. Interestingly, protease accessibility and biotinylation experiments indicated that an unusually large fraction of the lipoproteins are cell-surface exposed. We also identified three proteins that are members of a novel family of autotransporters, multiple potential type I protein secretion systems, and proteins that appear to be components of a type VI secretion apparatus. The secretome consisted of lipoproteins and other proteins that might be substrates of the putative type I or type VI secretion systems. Our proteomic studies show that B. fragilis differs considerably from well-studied Gram-negative bacteria such as Escherichia coli in both the spectrum of OM proteins that it produces and the range of secretion strategies that it utilizes.

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Bacterioides fragilis: Production and Sensitivity to Bacteriocins

Cited by 4 publications

References 13 publications

Non-toxigenic Bacteroides fragilis (NTBF) administration reduces bacteria-driven chronic colitis and tumor development independent of polysaccharide A

Non-toxigenic Bacteroides fragilis (NTBF) administration reduces bacteria-driven chronic colitis and tumor development independent of polysaccharide A

An antimicrobial protein of the gut symbiont Bacteroides fragilis with a MACPF domain of host immune proteins

Analysis of the Outer Membrane Proteome and Secretome of Bacteroides fragilis Reveals a Multiplicity of Secretion Mechanisms

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