Bacteriocins Applied to Food Packaging Materials to Inhibit Listeria monocytogenes on Meats

Abstract

Bacteriocins in powders were produced from milk-based media and applied to food packaging films. Nisin and pediocin ''powders'' were retained in casings during dialysis. Antilisterial casings were prepared by internal coating with pediocin. Antilisterial activity applied in powdered form was retained during processing and retained on contact food packaging surfaces. Pediocin powder was applied to plastic packaging bags at 7.75 µg/cm 2 . Meats and poultry samples were inoculated with Listeria monocytogenes. The bags coated with pediocin powder completely inhibited growth of inoculated L. monocytogenes through 12 wk storage at 4ЊC. Applying bacteriocins to food packaging films is an effective approach to reduce L. monocytogenes contamination in meats and poultry.

Antibacterial Activities of Nisin Z Encapsulated in Liposomes or Produced In Situ by Mixed Culture during Cheddar Cheese Ripening

“…Nisin Z causes an immediate loss of cell K ϩ , phosphate, and ATP in L. monocytogenes, suggesting the unselectivity of these pores (2). In addition, the bactericidal effect of nisin through pore formation has been found to be an energy-and transmembrane electrical potential-dependent process in the absence of lipid II (47,54,62). When lipid II is available, nisin can disturb the barrier function of membrane at nanomolar concentrations, compared to micromolar range without lipid II (62).…”

“…In the present study, immobilization of nisin Z on liposome membranes appeared to protect its activity and improve its availability and distribution in the cheese matrix. Adsorption of bacteriocins to various surfaces with retention of activity may be successfully achieved (10,11,21,47). Membrane-immobilized nisin or surfaces could provide several advantages as a nisin delivery system, such as reducing the amount of nisin that would be used and improving its stability (20,58).…”

Postharvest intervention technologies for safety enhancement of meat and meat based products; a critical review

“…Moreover, niacin give good results in decontaminating meat surface when applied in combination with alginate-based edible coatings. Accordingly in a study, Ming et al (1997) reported that sausages can be preserved by treating their inner surface with bacteriocins. They also applied niacin and pediocin to inner side of packaging materials and noticed a significant reduction for Listeria monocytogenes in breast turkey meat, ham and beef under refrigerated storage.…”

Substances with Antibacterial Activity in Edible Films – A Review

“…Another investigated bacteriocin was pediocin which was introduced into the cellulose fi lm. The foil with its addition was demonstrated to completely inhibit the growth of L. monocytogenes bacteria [Ming et al, 1997]. Equally promising results were achieved in a study conducted with a pullulan fi lm enriched with bacteriocin, namely with sakacin A.…”