Bacteriocin-producing Enterococcus faecalis KT2W2G isolated from mangrove forests in southern Thailand: Purification, characterization and safety evaluation

H‐Kittikun, Aran; Bíscola, Vanessa; El-Ghaish, Shady; Jaffrès, Emmanuel; Dousset, Xavier; Pillot, Guillaume; Haertlé, Thomas; Chobert, Jean‐Marc; Hwanhlem, Noraphat

doi:10.1016/j.foodcont.2014.12.037

Cited by 42 publications

(11 citation statements)

References 72 publications

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“…In the case of storage at 30°C and 37°C, the activity of bacteriocin decreased after 24 h and completely abolished after 1 week at 45°C ( Figure 4C). In a similar study, the activity of bacteriocin PPB KT2W2G produced by Enterococcus faecalis KT2W2G was fully maintained after 8 weeks of storage at -20, 4 and 37°C (H-Kittikun et al, 2015). These authors reported that the stability of bacteriocin activity at different temperatures during storage depend on the kind of bacteriocin.…”

Section: Characterization Of Bacteriocinmentioning

confidence: 77%

Optimization and some characteristics of bacteriocin produced by Enterococcus sp. CM9 collected from Mauritanian Camel milk

Benmouna

Karam

2018

Emir J Food Agric

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The use of bacteriocin as a bioconservation is very promising in the agri-food industry to substitute the chemical preservatives. The production of bacteriocin by lactic acid bacteria (LAB) depending on various conditions, the purpose of the present work was to assess the effect of some ingredients of medium on the bacteriocin production. Twenty-four strains of LAB of the genera Leuconostoc, Lactobacillus, and Enterococcus, isolated from camel milk, were tested for antibacterial activity and bacteriocin production. Among these strains, three isolates showed bacteriocinogenic activity, two Enterococcus strains (bacteriocin CM9, bacteriocin CM18), and one Leuconostoc strain (bacteriocin CHBK310). The bacteriocin produced by Enterococcus sp CM9 was subject of optimisation study. The modified MRS with nitrogen sources in media containing tryptone, meat extract and yeast extract and with potassium sources in media containing 2% of K2HPO4 allowed higher activity with inhibition zone of 10 mm and this production remained stable at incubation temperatures from 25 to 40°C with an inhibition zone which reached 12 mm at 25°C. The diffusion of this bacteriocin in the neutrality condition, allowed a better evaluation of its inhibitory effect. The activity of the bacteriocin was not affected, neither by acidic or basic pH nor by heat treatments, its activity remaining stable even after 2 weeks at -20 and 4°C. The bacteriocin CM9 belonged to class II, had a molecular weight of 7.6 kDa. These results indicated that the growth and diffusion conditions have a role in the bacteriocin production and its activity.

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Section: Characterization Of Bacteriocinmentioning

confidence: 77%

Optimization and some characteristics of bacteriocin produced by Enterococcus sp. CM9 collected from Mauritanian Camel milk

Benmouna

Karam

2018

Emir J Food Agric

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“…For the homopolymeric type EPS production, a single gene described as gtf/ftf is required (Walter et al 2008), whereas an eps gene cluster is responsible for the heteropolymeric EPS production in which the priming glycosyltransferase gene (p-gtf) initiates the production of the EPS repeating unit (Horn et al 2013). In addition to the cell surface associated functional characteristics of lactobacilli, production of antimicrobial components plays also crucial roles in probiotic action (Stern et al 2006) as well as in food preservation (H-Kittikun et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains

et al. 2015

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The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces. Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4. Analysis of EPS production levels showed significant alterations among strains tested. Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production. Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level. An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels. This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.

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“…Several other studies have reported that the antibiotics production corresponded to stationary growth phase of bacteria from 48-72 h [19,20]. While Kittikun [21,22]. The result suggested that antibiotics can be produced at different growth phase of bacteria.…”

Section: Production Of Antibioticmentioning

confidence: 93%

PHYLOGENETIC ANALYSIS OF 16S rRNA GENE FRAGMENT OF ANTIBIOTIC-PRODUCING PLS 76 ISOLATE

Suzanni

Yolanda

Saidi

et al. 2018

J. Nat.

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Exploitation of extremophiles as novel bioactive compounds sources has been increasing. The aim of this study was to evaluate the activity and the class of antibiotic produced by a thermo-halophilic isolate PLS 76, as well as to identify the genotype of the isolate. The activity was determined by a disc diffusion method, while the antibiotic class was determined qualitatively by chemical reactions using ninhydrin, iodine vapour and potassium iodine. The genotype was determined by sequencing the 16S rRNA gene fragment and the phylogenetic tree from the sequence data. The results showed that PLS 76 was a Gram-negative bacterium and able to produce polypeptide antibiotic, which showed a slight activity on E. coli and S. aureus. Sequence alignment of the 16S rRNA gene fragment showed that PLS 76 was most related to Geobacillus kaustophilus. These results may be used to utilise the isolated for further antibiotic study.

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Bacteriocin-producing Enterococcus faecalis KT2W2G isolated from mangrove forests in southern Thailand: Purification, characterization and safety evaluation

Cited by 42 publications

References 72 publications

Optimization and some characteristics of bacteriocin produced by Enterococcus sp. CM9 collected from Mauritanian Camel milk

Optimization and some characteristics of bacteriocin produced by Enterococcus sp. CM9 collected from Mauritanian Camel milk

Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains

PHYLOGENETIC ANALYSIS OF 16S rRNA GENE FRAGMENT OF ANTIBIOTIC-PRODUCING PLS 76 ISOLATE

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