Bacteriocin diversity inBacillus sphaericus

Abstract

Nondenaturing polyacrylamide gel electrophoresis revealed the presence of diversity among bacteriocins produced by strains of Bacillus sphaericus. Bacteriocin bands of six strains (pathogenic and non pathogenic) were found to be located just below the stacking gel. However, in two other strains (1 pathogenic and 1 collection strain) more than one protein band with bacteriocin activity were seen in the middle of resolving gel. In bacteriocin-treated cultures, electron-microscopy studies revealed the growth of lysedswollen ghost cells, and loss of viability among sensitive strains.

Bacteriocinogenic Lactobacillus plantarum ST16Pa isolated from papaya (Carica papaya) — From isolation to application: Characterization of a bacteriocin

“…Some bacteriocins are also active against certain Gram negative bacteria, such as Escherichia coli and Salmonella Thyphimurium (Gong, Meng, & Wang, 2010;Todorov & Dicks, 2005b) and interestingly against Campylobacter jejuni, the major cause of gastroenteritis worldwide (Stern et al, 2006). More than 300 different bacteriocins have been described for the genera Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Enterococcus (Çetinkaya, Osmanağaoğlu, Food Research International 44 (2011) 1351-1363& Çökmüş, 2003De Vuyst & Vandamme, 1994;Olasupo, 1998;Osuntoki, Gbenle, & Olukoya, 2003). They are generally low molecular weight proteins that gain entry into target cells by binding to cell surface receptors.…”

Partial characterization of bacteriocin AMA-K, produced by Lactobacillus plantarum AMA-K isolated from naturally fermented milk from Zimbabwe

“…Bacteriocins of LAB are deWned as ribosomally synthesized proteins or protein complexes usually antagonistic to genetically closely related organisms (De Vuyst & Vandamme, 1994;Klaenhammer, 1988;Nes & Johnsborg, 2004). More than 300 diVerent bacteriocins have been described for the genera Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Enterococcus (Çetinkaya, Osmanafaoflu, & Çökmüo, 2003;De Vuyst & Vandamme, 1994;Olasupo, 1998;Osuntoki, Gbenle, & Olukoya, 2003).…”

Isolation and identification of bacteriocinogenic strain ofLactobacillus plantarumwith potential beneficial properties from donkey milk

“…More than 300 different bacteriocins have been described for the genera Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Enterococcus (De Vuyst and Vandamme ; Olasupo ; Çetinkaya et al . ; Osuntoki et al . ).…”