Bacteriocin as Weapons in the Marine Animal-Associated Bacteria Warfare: Inventory and Potential Applications as an Aquaculture Probiotic

Desriac, Florie; Defer, Diane; Bourgougnon, Nathalie; Brillet, Benjamin; Chevalier, Patrick Le; Fleury, Yannick

doi:10.3390/md8041153

Cited by 141 publications

(94 citation statements)

References 141 publications

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“…Several studies suggest that production of bacteriocins and organic acids (mainly lactic acid) are the main weapons of probiotic LAB of fish and aquatic origin to fight against pathogens of relevance for aquaculture [61,62], similarly as reported for human and farm animals probiotic LAB [62,63]. With regard to this, from the 75 lactococci analyzed in this study, only 9 (12 %) strains were identified as bacteriocin producers.…”

Section: Discussionmentioning

confidence: 72%

Safety assessment, genetic relatedness and bacteriocin activity of potential probiotic Lactococcus lactis strains from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment

Araújo

Muñoz-Atienza

Ramirez

et al. 2015

Eur Food Res Technol

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in three well-defined and highly divergent groups (31.0 % similarity). Nine (12 %) L. lactis strains were identified as bacteriocin producers. The bacteriocin produced by L. lactis subsp. cremoris WA2-67 was purified, and mass spectrometry and DNA sequencing revealed its identity to nisin Z (NisZ). Altogether, the results allowed the identification of 34 (45.3 %) putatively safe lactococci, including three bacteriocinogenic strains. This is the first description of tet(K), tet(O) and tet(T) in L. lactis. Moreover, ERIC-PCR fingerprinting has been shown for the first time as a valuable tool for genetic profiling of L. lactis strains from aquatic origin. A NisZ-producing L. lactis strain has been isolated for the first time from an aquatic environment, and this strain may be considered as a potential probiotic for rainbow trout farming for improving the quality and safety of fish and fish products.

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Section: Discussionmentioning

confidence: 72%

Safety assessment, genetic relatedness and bacteriocin activity of potential probiotic Lactococcus lactis strains from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment

Araújo

Muñoz-Atienza

Ramirez

et al. 2015

Eur Food Res Technol

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“…In this study, the 8 P. acidilactici strains displayed a broad antimicrobial spectrum against fish pathogens, which could be due to different antimicrobial metabolites such as organic acids (mainly lactic acid), hydrogen peroxide, diacetyl, acetaldehyde, and/or bacteriocins, with the organic acids and bacteriocins being the key weapons in inhibiting pathogens of relevance for aquaculture (Gillor et al 2008, Desriac et al 2010. In this respect, 6 out of 8 P. acidilactici strains were found to be bacteriocin producers, based on the proteinaceous nature and heat stability of the antimicrobial agent, which is in agreement with previous studies, where a remarkably high number of LAB displaying antimicrobial activity against fish pathogens were potential bacteriocin producers (Muñoz-Atienza et al 2013, Gómez-Sala et al 2015.…”

Section: Discussionmentioning

confidence: 92%

Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss) feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity

Araújo¹,

Muñoz-Atienza²,

Poeta³

et al. 2016

Dis. Aquat. Org.

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The use of lactic acid bacteria (LAB) as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. The objectives of this work were (1) the in vitro safety assessment of 8 Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae; (2) the evaluation of their genetic relatedness; (3) the study of their antimicrobial/bacteriocin activity against fish pathogens; and (4) the biochemical and genetic characterization of the bacteriocin produced by the strain displaying the greatest antimicrobial activity. Concerning the safety assessment, none of the pediococci showed antibiotic resistance nor produced hemolysin or gelatinase, degraded gastric mucin, or deconjugated bile salts. Four strains (50%) produced tyramine or putrescine, but the corresponding genes were not amplified by PCR. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting allowed clustering of the pediococci into 2 well-defined groups (68% similarity). From the 8 pediococci displaying direct antimicrobial activity against at least 3 out of 9 fish pathogens, 6 strains (75%) were identified as bacteriocin producers. The bacteriocin produced by P. acidilactici L-14 was purified, and mass spectrometry and DNA sequencing revealed its identity to pediocin PA-1 (PedPA-1). Altogether, our results allowed the identification of 4 (50%) putatively safe pediococci, including 2 bacteriocinogenic strains. ERIC-PCR fingerprinting was a valuable tool for genetic profiling of P. acidilactici strains. This work reports for the first time the characterization of a PedPA-1-producing P. acidilactici strain isolated from an aquatic environment (rainbow trout larvae), which shows interesting properties related to its potential use as a probiotic in aquaculture.

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“…Farming industries, such as the poultry industry [21,69] and aquaculture industry [70], look for more efficient ways to treat their stock. Interest in human applications comes from scientists and companies that realize the global health and economic potential of bacteriocin therapeutics.…”

Section: Discussionmentioning

confidence: 99%

The therapeutic potential of bacteriocins as protein antibiotics

Behrens

Six

Walker

et al. 2017

Emerging Topics in Life Sciences

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The growing incidence of antibiotic-resistant Gram-negative bacterial infections poses a serious threat to public health. Molecules that have yet to be exploited as antibiotics are potent protein toxins called bacteriocins that are produced by Gram-negative bacteria during competition for ecological niches. This review discusses the state of the art regarding the use for therapeutic purposes of two types of Gram-negative bacteriocins: colicin-like bacteriocins (CLBs) and tailocins. In addition to in vitro data, the potency of eight identified CLBs or tailocins has been demonstrated in diverse animal models of infection with no adverse effects for the host. Although the characteristics of bacteriocins will need further study, results obtained thus far regarding their in vivo potency, immunogenicity and low levels of resistance are encouraging. This leads the way for the development of novel treatments using bacteriocins as protein antibiotics.

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Bacteriocin as Weapons in the Marine Animal-Associated Bacteria Warfare: Inventory and Potential Applications as an Aquaculture Probiotic

Cited by 141 publications

References 141 publications

Safety assessment, genetic relatedness and bacteriocin activity of potential probiotic Lactococcus lactis strains from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment

Safety assessment, genetic relatedness and bacteriocin activity of potential probiotic Lactococcus lactis strains from rainbow trout (Oncorhynchus mykiss, Walbaum) and rearing environment

Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss) feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity

The therapeutic potential of bacteriocins as protein antibiotics

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