Bactericidal properties of disinfectant «Enzidez»

Abstract

Disinfection as a component of veterinary and sanitary measures ensures the well-being of livestock, increasing of animal productivity and obtaining safe raw materials. Therefore, the development of disinfectants that have a wide range of antimicrobial action and at the same time affect bacterial biofilms is relevant. The aim of the study was to determine the minimum bactericidal concentration, phenolic coefficient and protein index of the new disinfectant "Enzidez". Museum strains of test cultures of E. coli (055K59 №3912 / 41), S. aureus (ATCC 25923) and P. aeruginosa (27/99) were used in the experiments. The minimum bactericidal concentration of Enzidez disinfectant solutions, the phenolic ratio and the protein index were determined by generally accepted suspension methods. It was found that the microorganisms E. coli and P. aeruginosa were more sensitive to the disinfectant "Enzidez" compared to S. aureus. By exposure for 15 min the minimum bactericidal dilution against S. aureus was on average 1.9 times (p <0.05) higher than the bactericidal dilution against E. coli and 3.8 times (p <0.05) in relation to P. aeruginosa. By 30-minute exposure the difference between the minimum bactericidal dilution against S. aureus and against E. coli and P. aeruginosa was 1.3 and 2.7 times less (p <0.05), respectively. Thus, with increasing exposure time from 15 to 30 minutes the concentration of the agent for the destruction of gram-positive and gram-negative microorganisms decreases significantly. It was found that the bactericidal activity of the disinfectant "Enzidez" against the strain S. aureus №ATCC 25923 was on average 12.7 times stronger than the bactericidal action of the phenol solution. The bactericidal activity of Enzides against E. coli and P. aeruginosa strains was 24.0 and 28.8 times stronger, respectively compared with the action of phenol. The presence of protein (blood serum) in the medium leads to a slight decrease in the bactericidal activity of Enzides solutions. In particular, the bactericidal activity of Enzides against S. aureus is reduced by an average of 1.4 times, provided the presence of 10% of serum in the medium. The bactericidal activity of Enzides against E. coli and P. aeruginosa in the presence of protein decreased by 1.35 and 1.45 times, respectively. Thus, the disinfectant "Enzidez" is quite active even in the presence of protein in the medium.