Bactericidal Effect of Direct and Filtered Ultraviolet C Through Transparent Plastic Sheet on Gram Negative Bacilli –An in- Vitro Study

Abstract

Wound infection (with both Gram-positive and Gram-negative organisms) is a major problem in delayed wound healing. Persistence of these organisms in the wound delays considerably the healing time and increases length of hospital stay. Ultraviolet-C has shown to effectively eliminate these organisms (bactericidal effect) in few laboratory, animal and human studies and has shown promising role in promoting wound healing. Several wound dressing methods have been used to accelerate the rate of wound healing. Among them, Limited Access Dressing (LAD) utilizes intermittent negative pressure dressing using a transparent plastic cover combined with moist wound healing principle, minimizes need for regular change of dressing. Thus, the present study was conducted to explore the in-vitro bactericidal effect of direct and filtered UVC through transparent plastic sheet on Gramnegative bacilli.

Efficacy of ultraviolet C light at sublethal dose in combination with antistaphylococcal antibiotics to disinfect catheter biofilms of methicillin-susceptible and methicillin-resistant <em>Staphylococcus aureus</em> and <em>Staphylococcus epidermidis</em> in vitro

“…Several in vitro studies have reported the susceptibility of multidrug-resistant bacteria to inactivation by UVC 9–12. These bacteria include methicillin-resistant Staphylococcus aureus (MRSA), coagulase-negative Staphylococcus , Streptococcus pyogenes , Enterococcus faecalis, Pseudomonas aeruginosa , and Mycobacteria 10–12.…”

Effect of Low-Level Laser Therapy and Ultraviolet-C Irradiation on Partial-Thickness Burn Wound Healing: A Randomized Controlled Trial

“…Effects include induction of releasable factors normally expressed during healing [38,39], modification of growth factor receptors [38,40], accelerated DNA synthesis, fibronectin release from fibroblasts [41], and epidermal cell proliferation [38]. The killing of common wound pathogens and antibiotic-resistant organisms has also been shown [18,42]. The germicidal properties of UV light have been known, approximately, for a century.…”

Corrosión atmosférica acelerada en una fundición gris ASTM A48

“…Para lo anterior, se procedió a sumergir cupones de fundición gris, en dos tipos de agua por separado. El primero, agua de mar natural de las costas de Antofagasta Chile; y el segundo, esta misma solución, pero expuesta a radiación UV-C. Esta segunda solución corresponde a una que presenta ausencia de bacterias, de acuerdo al efecto bactericida de los rayos UV-C [27]. Tras 14 (días) de exposición a las soluciones las muestras fueron retiradas, limpiadas y se realizó una inspección con electrones secundarios en SEM.…”