Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study

Dai, Shaotao; Xiao, Gang; Dong, Ning; Liu, Fei; He, Shi; Guo, Qingbin

doi:10.1186/s12903-018-0611-6

Cited by 23 publications

(22 citation statements)

References 23 publications

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“…Previous studies on the antimicrobial effects of diode laser irradiation were mainly conducted in medicine and were focused on pathogenic bacteria such as Enterococcus faecalis and Escherichia coli. Nevertheless, higher values of average laser power than in this paper were most often applied (Beer et al, 2011;Asnaashari et al, 2016;Olivi et al, 2016;Dai et al, 2018), with similar ones applied less often (Tosun et al, 2012;Sadık et al, 2013).…”

Section: Discussionmentioning

confidence: 45%

Application of a Medical Diode Laser (810 nm) for Disinfecting Small Microbiologically Contaminated Spots on Degraded Collagenous Materials for Improved Biosafety in Objects of Exceptional Historical Value From the Auschwitz-Birkenau State Museum and Protection of Human Health

2020

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The research aim was to optimize the operating parameters of a diode laser irradiation for the effective disinfection of degraded collagenous materials. Historical leather shoes stored at the Auschwitz-Birkenau State Museum in Oświęcim (Poland) were the main study objects. Surfaces of contaminated small spots occurring on the degraded materials were sampled with moistened swabs and microbiologically examined using the molecular techniques MALDI-TOF MS, 16S rRNA, and NGS sequencing. The surfaces were colonized by bacteria with 106 CFU/100 cm2 and 104 CFU/100 cm2 by fungi, on average. Microorganisms of the genera Bacillus and Penicillium were predominant. The effectiveness of the laser treatment was assessed for the new and degraded collagenous materials against isolated environmental strains using four variants of exposure time and number of repetitions. 0.3 W/CW 2 × 2 min variant was the most effective and also did not noticeably change the color of the treated samples. The variant caused a reduction in the numbers of microorganisms by 96–100%. After 1 month, four types of leather were subjected to comprehensive physico-chemical analyses. SEM and FTIR techniques confirmed that laser irradiation in the selected optimal variant did not affect the surface morphology and collagen structure, while XPS technique enabled detection of subtle changes in non-historical protective coatings on the surfaces of tested degraded historical materials.

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Section: Discussionmentioning

confidence: 45%

Application of a Medical Diode Laser (810 nm) for Disinfecting Small Microbiologically Contaminated Spots on Degraded Collagenous Materials for Improved Biosafety in Objects of Exceptional Historical Value From the Auschwitz-Birkenau State Museum and Protection of Human Health

2020

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“…The diode laser uses the solid semiconductor of aluminum gallium arsenide as an active medium operating in the near-infrared spectral region, between 808 and 980 nm. 35,36 The penetration of diode laser irradiation to a depth of up to 1000 μm into dentinal tubules could be responsible for its strong bactericidal effect. On the other hand, chemical disinfectants can only reach up to 100 μm into the surrounding dentinal tubules, whereas the bacteria can penetrate and remain protected over 1000 μm in the deeper layers of dentin.…”

Section: Discussionmentioning

confidence: 99%

“…can cause irreversible injury to the periodontal tissues. 36 Gutknecht et al demonstrated that diode laser irradiation for 5 seconds, with 10 seconds of resting time, should be considered to avoid a temperature rise to an undesired level. 39 Schoop et al also drew similar conclusions that a diode laser showed the lowest temperature increases compared with other laser devices and was suitable for the disinfection of root canals.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of the Efficacy of Chlorhexidine, Sodium Hypochlorite, the Diode Laser and Saline in Reducing the Microbial Count in Primary Teeth Root Canals – An In Vivo Study

Walia¹,

Goswami²,

Mishra³

et al. 2019

J Lasers Med Sci

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Please cite this article as follows: Walia V, Goswami M, Mishra S, Walia N, Sahay D. Comparative evaluation of the efficacy of chlorhexidine, sodium hypochlorite, the diode laser and saline in reducing the microbial count in primary teeth root canals -an in vivo study. Abstract Introduction:One of the primary goals of pulpectomy is to decrease the sum total of microorganisms and disinfect the tooth root canal system. To achieve this, mechanical preparation, irrigation, disinfection and obturation of the root canal is necessary. The present study was set out to evaluate the difference in the antibacterial efficacy of primary teeth root canals either irrigated with chlorhexidine, saline, and sodium hypochlorite or irradiated with a soft tissue diode laser (980 nm, Photon Plus, Zolar Tech & Mfg Co. Inc, Ontario, Canada). Methods: Sixty primary teeth of children requiring pulpectomy were divided into 4 groups of 15 each, group 1 (2% chlorhexidine), group 2 (1% sodium hypochlorite), group 3 (laser irradiation) and group 4 (saline). Pulp tissue was extirpated from the canals and the samples were collected using sterile absorbent paper points. After cleaning and shaping, the root canals of the teeth in each group were irrigated using sodium hypochlorite, chlorhexidine, and saline or were irradiated with the laser. The samples were obtained again and sent for microbiological examination. Results: The colony-forming unit (CFU) counts from pre-disinfection sample served as a baseline for comparisons throughout the study. The mean bacterial colony counts of all the isolated bacteria reduced after irrigation or irradiation. Intergroup comparisons showed no significant difference when groups 1, 2, and 3 were compared to each other (P > 0.05). However, a significant difference was seen when groups 1, 2, 3 were compared to group 4 (P < 0.05). Conclusion: Two percent chlorhexidine, 1% sodium hypochlorite and laser irradiation succeeded in reducing the root canal infection. Hence, diode laser irradiation may be a possible supplement to existing protocols for disinfecting the root canal system.

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“…2 Numerous previous studies have been conducted to evaluate the bactericidal effect of various wavelengths of lasers, but most of these studies were conducted on dentine slices of 100, 300, 500, and 1000 μm. [5][6][7] They were conducted with dentine samples up to 1000 μm, therefore limiting our understanding as to the effects of lasers and biocides, with one study beyond 2000 μm. 8 This in vitro study aimed to compare the photobiomodulation effect of a 940 nm laser on Enterococcus faecalis through varying thicknesses of human root dentin slices.…”

Section: Introductionmentioning

confidence: 99%

The Photobiomodulation Effect of 940nm Laser Irradiation on Enterococcus faecalis in Human Root Dentin Slices of Varying Thicknesses

2021

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Introduction: An increase in dentine thickness could result in an inadequate depth of laser energy penetration. This study aimed to evaluate the effect of a 940 nm laser on Enterococcus faecalis through varying thicknesses of human root dentin slices. Methods: Thirty-five dentin slices of root dentin with thicknesses ranging between 500 and 3000 µm were produced. Six experimental groups (500, 1000, 1500, 2000, 2500, and 3000 µm (n=5 each) were lased and the seventh, non-lased group served as the positive control with a dentine thickness of 2000 µm. The slices were inoculated with 2 µL of E. faecalis suspension of 1.5 × 108 E. faecalis cells/mL. All the lased slices were lased from the opposing side of the inoculation. A non-initiated 200 μm bare end fibre at the power of 1 W, in a continuous wave was used. Four doses of laser irradiation of 5 seconds with a side to side movement with the tip held at a 5º angle to the dentine slice were performed. The colony-forming units of E. faecalis were determined and the bacterial photobiomodulation effect analysed using one-way ANOVA with a Bonferroni and Holm post hoc test at a significance level of P>0.05. Results: There were statistical differences between the dentin slices of 500, 1000, and 1500 μm treated with the laser compared to the positive control (P<0.01). However, there were no statistical differences between the lased 2000 and 2500 μm slices compared to the positive control. There was significantly more photobiomodulation of the E. faecalis for the dentine slices of 3000 μm than the positive control (P<0.01). Conclusion: Laser treatment through dentine slices of 2000 μm and thinner significantly reduced bacterial growth. The photobiomodulation effects started to occur in dentine slices thicker than 2500 μm compared to the positive control.

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Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study

Cited by 23 publications

References 23 publications

Application of a Medical Diode Laser (810 nm) for Disinfecting Small Microbiologically Contaminated Spots on Degraded Collagenous Materials for Improved Biosafety in Objects of Exceptional Historical Value From the Auschwitz-Birkenau State Museum and Protection of Human Health

Application of a Medical Diode Laser (810 nm) for Disinfecting Small Microbiologically Contaminated Spots on Degraded Collagenous Materials for Improved Biosafety in Objects of Exceptional Historical Value From the Auschwitz-Birkenau State Museum and Protection of Human Health

Comparative Evaluation of the Efficacy of Chlorhexidine, Sodium Hypochlorite, the Diode Laser and Saline in Reducing the Microbial Count in Primary Teeth Root Canals – An In Vivo Study

The Photobiomodulation Effect of 940nm Laser Irradiation on Enterococcus faecalis in Human Root Dentin Slices of Varying Thicknesses

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