Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows

Abstract

Staphylococcus aureus is an important pathogen for both humans and animals, and it has been an ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. Elimination of S. aureus with classic antibiotics is difficult, and the current study aimed to evaluate the efficacy of ethanolic extracts of propolis (EEP) against S. aureus cultivated in complex media or milk. EEP (0-0.5 mg ml(-1)) decreased growth of S. aureus in BHI media and 1 mg ml(-1) was bactericidal against washed cell suspensions (10(7) CFU ml(-1)). Propolis extracts also killed S. aureus cells resuspended in milk, but the bactericidal dose was at least 20-fold greater. Cultures that were transferred for at least 60 generations with sub-lethal doses of propolis did not change much their sensibility to EEP. Atomic force microscopy images revealed changes in morphology and cell size of S. aureus cells exposed to EEP (0.5 mg ml(-1)). Our results indicate that propolis extracts might be effective against mastitis-causing S. aureus strains in vivo, but milk constituents affect the inhibitory activity of propolis. Considering that propolis-resistance appears to be a phenotype not easily selected, the use of EEP combined or not with other antimicrobial agents might be useful for mastitis control in vivo.

Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture

“…However, for the selection of isolates that have a greater chance of being efficient in the process of colonization and growth promotion, the isolates have first to be identified with preliminary in vitro biochemical analysis (Pal et al [2001]; Berg et al [2002]; Bernardes et al [2010]).Taxonomy of selected microorganisms with biotechnological potential in agriculture is thus a key step and, for this reason, sequencing of the 16S rRNA has been successfully used to identify genera such as Bacillus (Porwal et al In [2009], Deepa et al [2010]), Rhizobium, Burkholderia (Ferreira et al [2011]), Enterobacter, Pantoea Serratia , among others (Tian et al [2009]).…”

Bee Products and Essential Oils as Alternative Agents for Treatment of Infections Caused by S. aureus

“…The authors noticed that in milk the bactericidal dose was at least 20-fold greater [50]. Interesting results were also published by Suleman and colleagues [51].…”

“…The global market size of propolis was about 2300 tons in 2015 (it is established that it will increase to 2900 tons in 2021), and Brazil is the largest production and exporting country of this product (htps://www.whatech.com/market-research/materials-chemicals/125806-world-propolis-industry-trends-share-size-2021-forecast-report). High anti-staphylococcal activity of propolis sourced from State of Paraná, in Brazil, was observed by PamplonaZomenhan and coworkers, the MIC 50 and MIC 90 for the 210 strains (162 MSSA and 48 MRSA) were both 1420 μg/mL [48]. The results of investigations performed by the groups of Fidoralisi [49] and Santana [50] indicated that propolis extracts might be efective against mastitis-causing S. aureus.…”

“…High anti-staphylococcal activity of propolis sourced from State of Paraná, in Brazil, was observed by PamplonaZomenhan and coworkers, the MIC 50 and MIC 90 for the 210 strains (162 MSSA and 48 MRSA) were both 1420 μg/mL [48]. The results of investigations performed by the groups of Fidoralisi [49] and Santana [50] indicated that propolis extracts might be efective against mastitis-causing S. aureus. The group of Fidoralisi observed reduction in S. aureus growth on average, 1.5 and 4 log 10 times at concentration of propolis 200 and 500 μg/mL, respectively.…”

Propolis Extract-PVA Nanocomposites of Textile Design: Antimicrobial Effect on Gram Positive and Negative Bacterias

“…It is seen that propolis nanostructure containing solutions prevents proliferation of Staphylococcus aureus colonies effectively but not of Acinetobacter baumannii and Pseudomonas aeruginosa [15]. This proves that propolis extract containing nanostructure is effective on gram positive bacteria, Staphylococcus aureus [16][17][18][19]. …”