Bactericidal action of tachyplesin I against oral streptococci

Masuda, Koji; Ohta, Michio; Ito, Mikako; Ohsuka, Shinji; Kaneda, Toshio; Kato, Naoya

doi:10.1111/j.1399-302x.1994.tb00038.x

Cited by 9 publications

(4 citation statements)

References 30 publications

(16 reference statements)

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“…Further exploration of these compounds may lead to the development of valuable medicines for treating, among others, erectogenic conditions [76][77][78], botulism [97,98], malignant neoplasms [114,116,222,223], autoimmune disorders [133], microbial [161,165,210,215,245] and parasitic infections [147], and chronic pain [207], as well as improved bacterial endotoxin detection [159] and innovative sunscreens [178]. These advances are much needed when considering the high global incidence of erectogenic conditions [250], autoimmune disorders [251], and cancer [252], the worldwide threat of bioterrorism [99], and the increasing resistance of microbial [253] and parasitic infections [254] to exisiting medications.…”

Section: Discussionmentioning

confidence: 99%

“…They presumably play a role in the protection of horseshoe crabs from invading microorganisms. Indeed, these compounds were found to inhibit the growth of both Gram-negative and -positive bacteria at low concentrations [161,164,165]. Due to their small size, positive net charge, and amphiphatic character, they are able to insert into bacterial membranes and to interact with the negatively charged lipopolysaccharides in bacterial membranes [160][161][162]164] such as lipopolysaccharides [166].…”

Section: Horseshoe Crab-derived Antibioticsmentioning

confidence: 99%

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Exploring the global animal biodiversity in the search for new drugs – Spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes

Mans¹

2017

J Transl Sci

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Drug discovery and development programs have historically mainly focused on plants with medicinal properties and the extensive knowledge of traditional healers about these plants. More recently, the vast array of marine invertebrates and insects throughout the world have been recognized as additional sources for identifying unusual lead compounds to obtain structurally novel and mechanistically unique therapeutics. The results from these efforts are encouraging and have yielded a number of clinically useful drugs. However, many arthropods other than insects -such as spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes -also produce hundreds of bioactive substances in their venom that may become useful in the clinic. Many of these chemicals are defensive and predatory weapons of these creatures and have been refined during millions of years of evolution to rapidly and with high specificity and high affinity shut down critical molecular targets in prey and predators. Exploration of these compounds may lead to the development of, among others, novel drugs for treating diseases caused by abnormalities in humans in the same (evolutionary conserved) molecular targets such as erectyle dysfunction, botulism, and autoimmune disorders, as well as the identification of novel antineoplastic, antimicrobial, and antiparasitic compounds. This paper addresses the importance of bioactive compounds from spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes to these advances.

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Section: Discussionmentioning

confidence: 99%

Section: Horseshoe Crab-derived Antibioticsmentioning

confidence: 99%

Exploring the global animal biodiversity in the search for new drugs – Spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes

Mans¹

2017

J Transl Sci

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“…Among these 17 alpha amino acid residues, 4 cysteine residues constitute two disulfide bonds that contribute to the hemolytic ability of TP I in blood cells [2]. Furthermore, research has shown that TP I is active against both Gram-positive and Gram-negative bacteria [3], fungi [4], viruses [5], and cancer cells [6]. The peptide also interacts with DNA and inhibits the synthesis of macromolecules.…”

Section: Introductionmentioning

confidence: 99%

Genomic and Functional Analysis of the Toxic Effect of Tachyplesin I on the Embryonic Development of Zebrafish

Zhao

Dai

Jin

2014

Computational and Mathematical Methods in Medicine

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Tachyplesin I (TP I) is an antimicrobial peptide isolated from the hemocytes of the horseshoe crab. With the developments of DNA microarray technology, the genetic analysis of the toxic effect of TP I on embryo was originally considered in our recent study. Based on our microarray data of the embryonic samples of zebrafish treated with the different doses of TP I, we performed a series of statistical data analyses to explore the toxic effect of TP I at the genomic level. In this paper, we first employed the hexaMplot to illustrate the continuous variation of the gene expressions of the embryonic cells treated with the different doses of TP I. The probabilistic model-based Hough transform was used to classify these differentially coexpressed genes of TP I on the zebrafish embryos. As a result, three line rays supported with the corresponding 174 genes were detected in our analysis. Some biological processes of the featured genes, such as antigen processing, nuclear chromatin, and structural constituent of eye lens, were significantly filtered with the smaller P values.

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“…Tachyplesin I exerts variable bactericidal activities against oral Gram-positive streptococciwhich are potential dental and mucosal pathogensin aerobic broth culture (13). Protegrin isoforms, including PG-1, D-PG-1, PG-2, PG-3 and PG-5, were highly microbicidal against A. actinomycetemcomitans and the Capnocytophaga spp.…”

mentioning

confidence: 99%

Killing of Fusobacterium nucleaturn, Porphyromonas gingivalis and Prevotella intermedia by protegrins

Miyasaki¹,

Iofel²,

Oren

et al. 1998

J of Periodontal Research

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Protegrins are broad spectrum antibiotic peptides isolated from porcine leukocytes. In this study, we (i) examine the sensitivity of Gram‐negative, anaerobic periodontal pathogens to synthetic protegrins; (ii) determine the relative potencies of protegrin congeners against these bacteria; and (iii) compare the potency of protegrins with other antibiotic peptides, including magainin MSI‐78, tachyplesin I, cecropin P1, human defensins HNP‐1‐3, and clavanin A. Synthetic l‐ and d‐enantiomers of protegrin 1 (PG‐1 and D‐PG‐1, respectively), and L‐enantiomers of protegrins 2, 3 and 5 (PG‐2, PG‐3 and PG‐5) were tested against Fusobacteriurn nucleatum, and black‐pigmented organisms including Porphyromonas gingivalis and Prevotella intermedia. Strains of both F. nucleatum and the black‐pigmented organisms were sensitive to PG‐1, and exhibited mean ED99 of 2.2‐2.3 μg/ml and 3.4‐9.9 μg/ml, respectively. The D‐form was statistically more potent than the L‐form against these oral anaerobes, and although this difference in potency is unlikely to be of decisive therapeutic significance, the d‐form may be of value given ability to resist microbial and host‐derived proteases. PG‐1 was more potent than magainin, tachyplesin, cecropin, defensins and clavanin under test conditions. Hypertonic saIt concentrations and heat‐inactivated serum were found to be inhibitory to the bactericidal activity of PG‐1. PG‐1 was found to induce morphologic alterations in the ultrastructural appearance of F. nucleatum consistent with damage to the bacterial membranes. We conclude that protegrins may be useful antimicrobial agents in therapy against Gram‐negative anaerobic bacteria believed to be involved in chronic, adult forms of periodontal infections.

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Bactericidal action of tachyplesin I against oral streptococci

Cited by 9 publications

References 30 publications

Exploring the global animal biodiversity in the search for new drugs – Spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes

Exploring the global animal biodiversity in the search for new drugs – Spiders, scorpions, horseshoe crabs, sea spiders, centipedes, and millipedes

Genomic and Functional Analysis of the Toxic Effect of Tachyplesin I on the Embryonic Development of Zebrafish

Killing of Fusobacterium nucleaturn, Porphyromonas gingivalis and Prevotella intermedia by protegrins

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