Bactericidal Action of Photodynamic Antimicrobial Chemotherapy (PACT) with Photosensitizers Used as Plaque-Disclosing Agents against Experimental Biofilm

Ishiyama, Kirika; Nakamura, Keisuke; Kanno, Taro; Niwano, Yoshimi

doi:10.4265/bio.21.187

Cited by 12 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a consequence, it is strongly advocated that RB-AlEW could be applied to treat dental biofilms. Since Ishiyama's study dealt with a single-species S. mutans biofilm, the effect on mixed-species biofilms should be examined in the near future in order to further ascertain the feasibility of the treatment's application in dentistry [41] ( Table 1).…”

Section: Electrolyzed Watermentioning

confidence: 99%

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

Diogo

Faustino

Neves

et al. 2019

JFB

View full text Add to dashboard Cite

Apical periodontitis is a biofilm-mediated disease; therefore, an antimicrobial approach is essential to cure or prevent its development. In the quest for efficient strategies to achieve this objective, antimicrobial photodynamic therapy (aPDT) has emerged as an alternative to classical endodontic irrigation solutions and antibiotics. The aim of the present critical review is to summarize the available evidence on photosensitizers (PSs) which has been confirmed in numerous studies from diverse areas combined with several antimicrobial strategies, as well as emerging options in order to optimize their properties and effects that might be translational and useful in the near future in basic endodontic research. Published data notably support the need for continuing the search for an ideal endodontic photosensitizer, that is, one which acts as an excellent antimicrobial agent without causing toxicity to the human host cells or presenting the risk of tooth discoloration. The current literature on experimental studies mainly relies on assessment of mixed disinfection protocols, combining approaches which are already available with aPDT as an adjunct therapy. In this review, several approaches concerning aPDT efficiency are appraised, such as the use of bacteriophages, biopolymers, drug and light delivery systems, efflux pump inhibitors, negative pressure systems, and peptides. The authors also analyzed their combination with other approaches for aPDT improvement, such as sonodynamic therapy. All of the aforementioned techniques have already been tested, and we highlight the biological challenges of each formulation, predicting that the collected information may encourage the development of other effective photoactive materials, in addition to being useful in endodontic basic research. Moreover, special attention is dedicated to studies on detailed conditions, aPDT features with a focus on PS enhancer strategies, and the respective final antimicrobial outcomes. From all the mentioned approaches, the two which are most widely discussed and which show the most promising outcomes for endodontic purposes are drug delivery systems (with strong development in nanoparticles) and PS solubilizers.

show abstract

Section: Electrolyzed Watermentioning

confidence: 99%

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

Diogo

Faustino

Neves

et al. 2019

JFB

View full text Add to dashboard Cite

show abstract

“…In contrast, photo-irradiated rose bengal in AlEW showed prominent bactericidal activity of viable bacterial count, suggesting that PACT with rose bengal in combination with AlEW could be applied to treat dental biofilm. In a previous study Ishiyama K. et al (2012) showed that of the three photo sensitizers tested, rose bengal exerted the highest bactericidal activity against planktonic S. mutans, most likely due to its high incorporation efficiency into bacterial cells [22,23]. 2015) revealed in their study that in patients with aggressive periodontitis (AgP) the use of amoxicillin and metronidazole resulted in a statistically significant reduction of matrix metalloproteinase 8 and 9 MMP-8 gingival crevicular fluid GCF levels at 3 and 6 months after treatment.…”

Section: Porphyrinsmentioning

confidence: 98%

A Study on Revealing Agents in the Context of Photodynamic Therapy in Dental Medicine - A Literature Review

Dascalu

Saroși²,

Moldovan³

et al. 2017

DDF

View full text Add to dashboard Cite

Photodynamic therapy (PDT) also known as Phototherapy or Photo chemotherapy involves the use of a photoactive dye (photosensitive) which is activated by exposure to light of a certain wavelength in the presence of oxygen. Dentists have to deal regularly with oral infections which untreated may cause systemic infections. Oral infections such as caries, periodontal infections and infections of the oral mucosa are available lighting so photodynamic therapy is particularly suitable for oral diseases [1,2]. Antimicrobial chemical treatment is nowadays widely used in the prophylaxis and treatment of inflammation-induced plaque with the risk of resistance development. The use of photodynamic therapy as a therapeutic approach can eliminate this risk, however. Studies in the literature show that both Gram-positive bacteria and Gram-negative bacteria are susceptible to the treatment. Among the advantages of using this method is counted rapid elimination of bacteria, minimal chances of developing bacterial resistance and safety in terms of host tissue and existing normal microflora in the oral cavity [3]. Antimicrobial photodynamic therapy (aPDT) by photochemical reaction uses light at a specific wavelength to activate a nontoxic photosensitizer (PS) in the presence of oxygen to produce cytotoxic products. There are different PSs used in dentistry including methylene blue (MB), toluidine blue O (TBO), indocyanine green (ICG) and curcumin [4].

show abstract

“…Modification of Gram-stain is rarely done, but discoveries for alternatives have been done in several recent researchers by using different dyes or stains, which result in higher contrast [6]. In other cases, novel staining methods were developed in dentistry to detect the presence of oral biofilm in the teeth and gums [10,18]. However, the experiments tend to use pure compounds and do not generally use foodcoloring dyes directly.…”

Section: Issn 2087-2852 E-issn 2338-1655mentioning

confidence: 99%

The Use of Food Coloring Dyes in Bacterial Staining

Risandiansyah

Arniyati

Nurita

et al. 2020

JELS

View full text Add to dashboard Cite

Staining creates a contrast between the cells and its surrounding, and enables the microscopic characteristics of bacterial cells to be easily visible and distinguished. However, staining often relies on dyes which are expensive, not readily available, or toxic. In this study, the use of food coloring dyes to stain bacteria was explored. We stained Grampositive bacteria (Bacillus sp. and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) using several food coloring dyes of different colors, which were purchased locally. After slide fixation, the dye was flooded on the bacterial smear and air-dried for up to 30 minutes and observed by using microscope before and after washing with water. The results of this study show that prior to washing, most food coloring dyes were able to stain bacterial cells. However, after washing, only pink and purple food coloring dyes were retained, showing pink colored cells. We suspected that erythrosine was the agent responsible for this result, and was able to show similar characteristics with erythrosine alone. This study concludes that food coloring dyes containing erythrosine can be used to stain bacterial cells indiscriminately.

show abstract

Bactericidal Action of Photodynamic Antimicrobial Chemotherapy (PACT) with Photosensitizers Used as Plaque-Disclosing Agents against Experimental Biofilm

Cited by 12 publications

References 19 publications

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

A Study on Revealing Agents in the Context of Photodynamic Therapy in Dental Medicine - A Literature Review

The Use of Food Coloring Dyes in Bacterial Staining

Contact Info

Product

Resources

About