Bacterial wilt of sweet potato caused by Ralstonia solanacearum in Taiwan

Chen, Yi-Jeng; Lin, Yi-Sheng; Chung, Wen‐Hsin

doi:10.1007/s10327-011-0353-7

Cited by 9 publications

(7 citation statements)

References 9 publications

(8 reference statements)

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“…In comparison to various hexoses, BS168 exhibited a consistent trend of a higher chemotactic response toward glucose (Figure 1a). This trend aligns with the significant glucose chemotaxis observed in the wild‐type (WT) BS strain MCLB2 (Yen, 2023). The mild chemotactic tendency observed in our study may be attributed to the relatively weaker biofilm‐forming ability of the specific BS strain utilized (McLoon et al, 2011).…”

Section: Resultssupporting

confidence: 78%

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The AT‐hook protein AHL29 promotes Bacillus subtilis colonization by suppressing SWEET2‐mediated sugar retrieval in Arabidopsis roots

Wu,

Yu,

Chiu

et al. 2023

Plant Cell & Environment

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Beneficial Bacillus subtilis (BS) symbiosis could combat root pathogenesis, but it relies on root‐secreted sugars. Understanding the molecular control of sugar flux during colonization would benefit biocontrol applications. The SWEET (Sugar Will Eventually Be Exported Transporter) uniporter regulates microbe‐induced sugar secretion from roots; thus, its homologs may modulate sugar distribution upon BS colonization. Quantitative polymerase chain reaction revealed that gene transcripts of SWEET2, but not SWEET16 and 17, were significantly induced in seedling roots after 12 h of BS inoculation. Particularly, SWEET2‐β‐glucuronidase fusion proteins accumulated in the apical mature zone where BS abundantly colonized. Yet, enhanced BS colonization in sweet2 mutant roots suggested a specific role for SWEET2 to constrain BS propagation, probably by limiting hexose secretion. By employing yeast one‐hybrid screening and ectopic expression in Arabidopsis protoplasts, the transcription factor AHL29 was identified to function as a repressor of SWEET2 expression through the AT‐hook motif. Repression occurred despite immunity signals. Additionally, enhanced SWEET2 expression and reduced colonies were specifically detected in roots of BS‐colonized ahl29 mutant. Taken together, we propose that BS colonization may activate repression of AHL29 on SWEET2 transcription that would be enhanced by immunity signals, thereby maintaining adequate sugar secretion for a beneficial Bacillus association.

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Section: Resultssupporting

confidence: 78%

“…The strain 168 is one of the domesticated lab strain with moderate biofilm formation (Gallegos‐Monterrosa et al, 2016). Ralstonia solanacearum RS7 was isolated from symptomatic tomato plants at Xinshe Dist., Taichung City, Taiwan (Yen, 2023).…”

Section: Methodsmentioning

confidence: 99%

The AT‐hook protein AHL29 promotes Bacillus subtilis colonization by suppressing SWEET2‐mediated sugar retrieval in Arabidopsis roots

Wu,

Yu,

Chiu

et al. 2023

Plant Cell & Environment

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“…Bacterial wilt disease caused by soil-borne Ralstonia solanacearum is one of the most devastating bacterial diseases in the tropics, subtropics, and some temperate areas with humid and hot summers, with phylotype IIB-1 strains that are acclimatized to even lower temperatures and have caused significant outbreaks in temperate areas in recent years [1,2]. R. solanacearum affects more than 200 plant species, including many solanaceous plants [3,4]. The yield loss of tomato varies up to 91%, and the losses vary from about 10% to 30% in tobacco and from 33% to 90% in potato [5].…”

Section: Introductionmentioning

confidence: 99%

“…Bacteria constantly oozed from the cut end of diseased stems when placed in water. R. solanacearum can be transmitted in various ways, such as root contact, irrigation water, machinery, or insects [3,4,7,8].…”

Section: Introductionmentioning

confidence: 99%

Selection, Formulation, and Field Evaluation of Bacillus amyloliquefaciens PMB01 for Its Application to Manage Tomato Bacterial Wilt Disease

et al. 2022

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Bacterial wilt caused by the soil-borne pathogen Ralstonia solanacearum is one of the most devastating diseases in solanaceous plants. No agrochemicals are available to manage bacterial wilt effectively. A Bacillus amyloliquefaciens strain designated PMB01 was recovered from the cabbage rhizosphere and was found to be capable of inhibiting the growth of R. solanacearum. The PMB01 strain was highly resistant to extreme pH, heat, high salt salinity, and various fungicides. In contrast, PMB01 was sensitive to copper-based compounds, streptomycin, and tetracycline. The efficacy of the PMB01 strain in suppressing R. solanacearum and bacterial wilt in tomatoes was significantly improved when the culture medium was supplemented with 1% (w/v) soybean meal. PMB01 was in a 500-liter tank for the pilot production, and the resultant broth could effectively reduce the severity of tomato bacterial wilt in greenhouse trials. The PMB01 fermentation broth was mixed with 10% corn starch and 30% maltodextrin to make a wettable powder (WP). PMB01 could survive in the wettable powder for more than two years without losing its antagonistic activity. In ten field trials, tomato plants treated with 50, 100, or 200-fold dilutions of PMB01 WP reduced bacterial wilt severity by more than 67% compared to the mock (water control) treatment. This work revealed that the effectiveness of the rhizobacterium PMB01 to antagonize R. solanacearum was greatly improved when the culture medium was supplemented with 1% (w/v) soybean meal, indicating that PMB01 is an ideal bio-agent candidate. A durable format suitable for storage was also developed. Similar concepts may be applied to other bio-agent candidates to improve their effectiveness in disease management.

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“…Vegetable sweet potato (VSP), Ipomoea batatas (L.) Lam., a type of sweet potato that generates many tender shoots but does not produce root tubers, is an important green vegetable source during summer or rainy season in East and South Asia (Lai, Lee, & Chen, ). Yields of VSP have been shown to be reduced by 30%–80% due to bacterial wilt (BW) in Taiwan during the past several years (Chen, Lin, & Chung, ). Propagation is by vine cuttings, and a recent study demonstrated that, Ralstonia solanacearum race 1 biovar 4 (R1bv4) can latently infect such cuttings and serve as the major inoculum source for introducing BW into Taiwan VSP fields (Chen, Lin, Tseng, & Chung, ).…”

Section: Introductionmentioning

confidence: 99%

Distribution and multiplication of Ralstonia solanacearum strain race 1 biovar 4 in vegetable sweet potato cuttings

Chen

Lin

Pan

et al. 2019

Journal of Phytopathology

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Ralstonia solanacearum (RS) race 1 biovar 4 (R1bv4), causal agent of bacterial wilt in vegetable sweet potato (VSP), is often latent in VSP vines and is important in introduction of the pathogen to newly planted fields. In this study, the effects of biological and environmental factors on the distribution and multiplication of R1bv4 in VSP tissues were examined. Based on stem‐injection inoculation, the R1bv4strain of NC01 could cause 49.0% and 33.0% wilting on VSP cultivars TN71 and WS, respectively. The populations of NC01 in diseased TN71 and WS were 108–109 cfu/g tissue at 28th day after inoculation. On the other hand, the R1bv4 could not cause symptom in cultivars of TN57 and VSPSL‐1 vine and the NC01 was confined to near the injection sites. Temperature tests indicated that NC01 could cause 28.0% and 14.0% wilting on cultivar TN71 at 28 and 20°C, respectively. Moreover, the populations of NC01in diseased plants were 1.6 × 109 and 7.9 × 108 cfu/g tissue at 28 and 20°C, respectively. The distribution of NC01 in VSP stem indicated that the isolation frequency of NC01 was lower than 31.0% in terminal shoots or erect stems and 45.0 to 100.0% in creeping stem after 8 wks planted in infested soil (106 cfu/g soil). The results demonstrated that terminal shoots or erect stems were not common carrier for transmitting R1bv4. Furthermore, two R1bv4 strains, NC01 and HsinT01, were examined the ability for latent infection on cv. TN71. The results revealed that NC01 and HsinT01 showed different ability of latent infection on cultivar TN71. NC01 had lower percentage (46.8% and 45.1%) than HsinT01 (93.4% and 75.3%) at 20 and 28°C.

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Bacterial wilt of sweet potato caused by Ralstonia solanacearum in Taiwan

Cited by 9 publications

References 9 publications

The AT‐hook protein AHL29 promotes Bacillus subtilis colonization by suppressing SWEET2‐mediated sugar retrieval in Arabidopsis roots

The AT‐hook protein AHL29 promotes Bacillus subtilis colonization by suppressing SWEET2‐mediated sugar retrieval in Arabidopsis roots

Selection, Formulation, and Field Evaluation of Bacillus amyloliquefaciens PMB01 for Its Application to Manage Tomato Bacterial Wilt Disease

Distribution and multiplication of Ralstonia solanacearum strain race 1 biovar 4 in vegetable sweet potato cuttings

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