Bacterial Wilt of Perilla Caused by<i>Pseudomonas solanacearum</i>and its Transmission

Hsu, Shih Tien; Hong, Wai-Foong; Tzeng, K. C.; Chen, Chin-Chih

doi:10.1094/pd-77-0674

Cited by 10 publications

(3 citation statements)

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“…4b). This result is in agreement with the report of Bhide (1948), Das and Chattopadhyay (1955) and Hsu et al (1993) and While Rath and Addy (1977).…”

Section: Casein Hydrolysissupporting

confidence: 94%

Characterization of Plant Pathogenic Ralstonia Solanacearum Causing Bacterial Wilt of Potato

Joshi,

Patel,

Parmar

et al. 2024

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After wheat, rice and maize, the potato (Solanum tuberosum L.) is considered one of the world's most significant food crops. Bacterial wilt or brown rot of potato, caused by Ralstonia solanacearum, is one of the most economically important diseases of potato. The purpose of this study was to characterize morphological, pathological and biochemical characterization of Ralstonia solanacearum. Total ten isolates of Ralstonia solanacearum were isolated on TZC agar medium from infected potato plant parts. All isolates of R. solanacearum showed fluidal, irregular and creamy white with pink center colony on TZC medium after 48 h of incubation were selected. The isolates of R. solanacearum showed a positive result for the pathogenicity test and produced wilting symptoms on potato plants (Kufri Pukhraj). The results of biochemical studies showed that all the ten isolates were gram-negative, rod-shaped and positive for KOH test, oxidase test, catalase test, production of hydrogen sulphide, casein hydrolization test and acid production test, but they showed negative reaction for indole test. R. solanacearum was identified as the pathogen causing potato wilt based on an isolation study (colony characteristics on TZC media), oozing test, pathogenicity test and various biochemical tests.

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“…4b). This result is in agreement with the report of Bhide (1948), Das and Chattopadhyay (1955) and Hsu et al (1993) and While Rath and Addy (1977).…”

Section: Casein Hydrolysissupporting

confidence: 94%

Characterization of Plant Pathogenic Ralstonia Solanacearum Causing Bacterial Wilt of Potato

Joshi,

Patel,

Parmar

et al. 2024

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“…Likewise, the isolates oxidized glucose with release of gas, this result also corroborates with those of Khetmalas [49], [50] who reported that R. solanacearum was positive in gas production in the presence of glucose. However, the results concerning the negative production of gaseous hydrogen sulphide (H2S) corroborate with those of Hsu et al [51] and [36] who carried out the same test on R. solanacearum. All the above-mentioned results categorically proved that the tomato plant isolates were the pathogen R. solanacearum.…”

Section: Gramsupporting

confidence: 83%

Morphological and Biochemical Characterization of Strains of Ralstonia solanacearum, Causal Agent of Bacterial Wilt in Tomatoes in Cameroon and Screening of Virulent Strains

Mountseng,

Mahot,

Membang

et al. 2024

ARRB

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Ralstonia solanacearum is a major constraint in tomato production. The aim of this work was to identify the different biovars and races of R. solanacearum which infect tomato plants in Cameroon and determine the most virulent in a gnotobiotic environment. Thirty (30) samples were collected in the field of 03 different agro-ecological zones considered as major tomato production area in Cameroon. The bacteria were isolated on modified Kelman solid medium, and identified based on morphological and biochemical characterization. The pathogenicity test was performed in a gnotobiotic environment by root inoculation. Twenty-nine (29) isolates showed colonies characteristic of virulent strains. Water, motility, catalase, KOH, Kovac oxidase, glucose reduction, and sucrose tests were positive. However, gram staining, spore production, arginine test and sulfate reduction were negative. The biovar tests carried out revealed the presence16.66% biovar 2 (bv 2) and 76.66% biovar 3(bv 3). The tobacco hypersensitivity tests carried out revealed the presence of two (02) races: race 1 and race 3 (majority). A predominance of race3 biovar 3 was observed in Cameroon. Results from pathogenicity test revealed that two (02) strains, FM6 (race 3 bv2) and BFo (race 3 bv 3) were high virulent, causing up to 100% loss of seedlings on the sixth day post-inoculation.

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“…The presence of this pathogen on several hundred species representing 44 families of plants has been recorded (4). In Taiwan, the pathogen has been reported to occur on tomato, tobacco, potato, sweet pepper, eggplant, peanut, birdof-paradise (40), strawberry, perilla (Perilla crispa) (22), castor bean, sesame, radish (26), anthurium (37), and eustoma (Eustoma grandiflorum L.) (5) plants. R. solanacearum strains represent a heterogeneous group that has been subdivided into five races on the basis of host range (3; I. W. Buddenhagen, L. Sequeira, and A. Kelman, abstr., Phytopathology 52:726, 1962) or five biovars based on the ability to oxidize certain disaccharides and hexose alcohols (19,20).…”

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confidence: 99%

Isolation of an Insertion Sequence from Ralstonia solanacearum Race 1 and Its Potential Use for Strain Characterization and Detection

Lee

Fan

Chiu

et al. 2001

Appl Environ Microbiol

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A new insertion sequence (IS), IS1405, was isolated and characterized from a Ralstonia solanacearum race 1 strain by the method of insertional inactivation of the sacB gene. Sequence analysis indicated that the IS is closely related to the members of IS5 family, but the extent of nucleotide sequence identity in 5 and 3 noncoding regions between IS1405 and other members of IS5 family is only 23 to 31%. Nucleotide sequences of these regions were used to design specific oligonucleotide primers for detection of race 1 strains by PCR. The PCR amplified a specific DNA fragment for all R. solanacearum race 1 strains tested, and no amplification was observed with some other plant-pathogenic bacteria. Analysis of nucleotide sequences flanking IS1405 and additional five endogenous IS1405s that reside in the chromosome of R. solanacearum race 1 strains indicated that IS1405 prefers a target site of CTAR and has two different insertional orientations with respect to this target site. Restriction fragment length polymorphism (RFLP) pattern analysis using IS1405 as a probe revealed extensive genetic variation among strains of R. solanacearum race 1 isolated from eight different host plants in Taiwan. The RFLP patterns were then used to subdivide the race 1 strains into two groups and several subgroups, which allowed for tracking different subgroup strains of R. solanacearum through a host plant community. Furthermore, specific insertion sites of IS1405 in certain subgroups were used as a genetic marker to develop subgroup-specific primers for detection of R. solanacearum, and thus, the subgroup strains can be easily identified through a rapid PCR assay rather than RFLP analysis.

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Bacterial Wilt of Perilla Caused byPseudomonas solanacearumand its Transmission

Cited by 10 publications

References 0 publications

Characterization of Plant Pathogenic Ralstonia Solanacearum Causing Bacterial Wilt of Potato

Characterization of Plant Pathogenic Ralstonia Solanacearum Causing Bacterial Wilt of Potato

Morphological and Biochemical Characterization of Strains of Ralstonia solanacearum, Causal Agent of Bacterial Wilt in Tomatoes in Cameroon and Screening of Virulent Strains

Isolation of an Insertion Sequence from Ralstonia solanacearum Race 1 and Its Potential Use for Strain Characterization and Detection

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