Bacterial wilt of ginger in Queensland: reappraisal of a disease outbreak

Hayward, Alice; Pegg, K. G.

doi:10.1007/s13313-012-0174-y

Cited by 10 publications

(6 citation statements)

References 9 publications

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“…Strains of the RSSC were initially sub-divided into five “races” based on host ranges, and into five “biovars” based on carbohydrate utilization [ 18 , 19 ]. Later, sequence analysis of the internal transcribed spacer (ITS) region of the 16S-23S rRNA gene, further divided the RSSC into four phylogenetic groups (“phylotypes” I to IV) which corresponded to the geographical origins of strains from Asia, America, Africa, and Indonesia, respectively [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Different Bacterial Wilt Resistant Eggplant Rootstocks for Grafting Tomato

Manickam

Chen

Sotelo-Cardona

et al. 2021

Plants

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Bacterial wilt (BW) is one of the most economically important diseases of tomato and eggplant in the tropics and subtropics, and grafting onto resistant rootstocks can provide an alternative and effective solution to manage soil-borne bacterial in these crops. This study was conducted to evaluate the BW resistance and agronomic potential of newly identified eggplant accessions as rootstocks for tomato grafting. Five BW resistant eggplant accessions (VI041809A, VI041943, VI041945, VI041979A, and VI041984) from the World Vegetable Center were evaluated as rootstocks for grafting with two different fresh market tomato cultivars (Victoria and TStarE) as scion under open field conditions in Taiwan. Graft compatibility using the tube grafting method as well as BW wilting percentage, disease index, fruit yield and quality parameters were assessed. All the rootstocks showed good graft compatibility (93% and above) and grafted plants showed low wilting percentage (0.0–20.0%) and disease index (0.0–20.8%) following inoculation with BW. Yield for the eggplant rootstock grafted tomato plants was higher compared to the non-grafted tomatoes and self-grafted tomato. Fruit quality was not affected by grafting, although some differences in antioxidant activities were observed. The new eggplant rootstocks can be considered as alternatives to the rootstocks currently used for commercial production of tomatoes during the hot-wet season.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Different Bacterial Wilt Resistant Eggplant Rootstocks for Grafting Tomato

Manickam

Chen

Sotelo-Cardona

et al. 2021

Plants

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“…It is present worldwide, threatening food safety of small producers in tropical and subtropical areas, especially in China, Bangladesh, Bolivia, and Uganda (Martin and French 1985;Muthoni et al 2012). However, because the pathogen disseminates easily to long distances via infected plant material (Hayward and Pegg 2013;Janse et al 2004), there has been a recent spread of the disease to temperate regions (Genin and Boucher 2004). R. solanacearum exhibits an extremely wide host range, causing bacterial wilt on more than 200 plant species, including commercially important crops such as potato, tomato, and banana (Hayward 1991).…”

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confidence: 99%

A Novel, Sensitive Method to Evaluate Potato Germplasm for Bacterial Wilt Resistance Using a LuminescentRalstonia solanacearumReporter Strain

Zuluaga¹,

Ferreira²,

Pianzzola³

et al. 2014

MPMI

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Several breeding programs are under way to introduce resistance to bacterial wilt caused by Ralstonia solanacearum in solanaceous crops. The lack of screening methods allowing easy measurement of pathogen colonization and the inability to detect latent (i.e., symptomless) infections are major limitations when evaluating resistance to this disease in plant germplasm. We describe a new method to study the interaction between R. solanacearum and potato germplasm that overcomes these restrictions. The R. solanacearum UY031 was genetically modified to constitutively generate light from a synthetic luxCDABE operon stably inserted in its chromosome. Colonization of this reporter strain on different potato accessions was followed using life imaging. Bacterial detection in planta by this nondisruptive system correlated with the development of wilting symptoms. In addition, we demonstrated that quantitative detection of the recombinant strain using a luminometer can identify latent infections on symptomless potato plants. We have developed a novel, unsophisticated, and accurate method for high-throughput evaluation of pathogen colonization in plant populations. We applied this method to compare the behavior of potato accessions with contrasting resistance to R. solanacearum. This new system will be especially useful to detect latency in symptomless parental lines before their inclusion in long-term breeding programs for disease resistance.Bacterial wilt caused by Ralstonia solanacearum is one of the world's most devastating bacterial diseases of plants (Peeters et al. 2013). It is present worldwide, threatening food safety of small producers in tropical and subtropical areas, especially in China, Bangladesh, Bolivia, and Uganda (Martin and French 1985;Muthoni et al. 2012). However, because the pathogen disseminates easily to long distances via infected plant material (Hayward and Pegg 2013;Janse et al. 2004), there has been a recent spread of the disease to temperate regions (Genin and Boucher 2004). R. solanacearum exhibits an extremely wide host range, causing bacterial wilt on more than 200 plant species, including commercially important crops such as potato, tomato, and banana (Hayward 1991). Wilting symptoms are caused by extensive multiplication of the bacterium in the xylem vessels, which hinders water flow in the plant. In addition, the bacterium can colonize asymptomatically several weeds that act as pathogen reservoirs (Genin and Denny 2012) and survive in waterways and soil for long periods (Caruso et al. 2005;Elphinstone 2005). These facts, together with the lack of resistant commercial varieties in any of its hosts , render the control of bacterial wilt very challenging.R. solanacearum is considered a species complex, divided into four phylotypes which roughly reflect their geographic origin: phylotype I groups strains from Asia, phylotype II from the Americas, phylotype III from Africa, and phylotype IV from Indonesia (Peeters et al. 2013). Each phylotype is subdivided into different sequevars which are clus...

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“…Nearly identical observations were made in China for biovar 4 ginger isolates where Chinese strains were not genetically distinguishable from ACH92 (r4‐bv4) isolated from Queensland (Xu et al ., ). This led to the theory that the Queensland isolate was an exotic strain originating from China, possibly through ginger consignments (Hayward & Pegg, ). MLST data further substantiated the limited intraracial diversity among biovar 3 isolates.…”

Section: Discussionmentioning

confidence: 99%

Host specificity and genetic diversity of race 4 strains of Ralstonia solanacearum

et al. 2014

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Ralstonia solanacearum race 4 isolates were obtained from Zingiberaceae plants in India during bacterial wilt outbreaks. Polyphasic phenotypic and genotypic analysis revealed intraracial diversity and dominance of biovar 3 over biovar 4. Biovar 3 strains were isolated from very severely wilted Zingiberaceae plants in the field and found to be present across diverse geographical, host and seasonal boundaries. It was hypothesized that these isolates belong to a single, 'fast wilting', lineage. Using one 'fast wilting' isolate in controlled inoculations, rapid wilt was observed in ginger within 5-7 days. Wilting was also observed in several other closely and distantly related hosts such as turmeric (Curcuma longa), aromatic turmeric (Curcuma aromatica), black turmeric (Curcuma caesia), sand ginger (Kaempferia galanga), white turmeric (Curcuma zeodaria), awapuhi (Zingiber zerumbet), greater galangal (Alpinia galanga), globba (Globba sp.), small cardamom (Elettaria cardamomum) and large cardamom (Ammomum subulatum) of the Zingiberaceae family, and in tomato (Solanum lycopersicum). Molecular analysis, including multiplex PCR-based phylotyping, sequence analysis of 16S rDNA, 16-23S intergenic spacer and the recN gene, and multilocus sequence typing, revealed minimal differences between fast wilting isolates, confirming that almost all belong to the same lineage. Biovar 4 was isolated from plants showing slow wilt progression and self-limiting wilting in restricted geographical locations instead, and was identified to be genetically distinct from the fast wilting biovar 3 isolates. To the authors' knowledge, this is the first report of host range and genetic analysis of R. solanacearum race 4 in India.

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Bacterial wilt of ginger in Queensland: reappraisal of a disease outbreak

Cited by 10 publications

References 9 publications

Evaluation of Different Bacterial Wilt Resistant Eggplant Rootstocks for Grafting Tomato

Evaluation of Different Bacterial Wilt Resistant Eggplant Rootstocks for Grafting Tomato

A Novel, Sensitive Method to Evaluate Potato Germplasm for Bacterial Wilt Resistance Using a LuminescentRalstonia solanacearumReporter Strain

Host specificity and genetic diversity of race 4 strains of Ralstonia solanacearum

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