Bacterial Volatile Compounds: Functions in Communication, Cooperation, and Competition

Netzker, Tina; Shepherdson, Evan M F; Zambri, Matthew; Elliot, Marie A.

doi:10.1146/annurev-micro-011320-015542

Cited by 71 publications

(53 citation statements)

References 101 publications

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“…The RL1 genome harbors genes related to the production of volatiles, exopolysaccharides and proteases, which are important in microbial communication, plant colonization and microbial detection by the host ( Flemming et al, 2016 ; Netzker et al, 2020 ). The chemotaxis protein CheY relevant for the transmission of sensory signals from the chemoreceptors to the flagella motors, which is additionally a microbe-associated molecular pattern (MAMP) ( Paul et al, 2010 ) was identified in the RL1 genome.…”

Section: Discussionmentioning

confidence: 99%

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

et al. 2021

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Stress tolerant, plant-associated bacteria can play an important role in maintaining a functional plant microbiome and protecting plants against various (a)biotic stresses. Members of the stress tolerant genus Rhodococcus are frequently found in the plant microbiome. Rhodococcus qingshengii RL1 was isolated from Eruca sativa and the complete genome was sequenced, annotated and analyzed using different bioinformatic tools. A special focus was laid on functional analyses of stress tolerance and interactions with plants. The genome annotation of RL1 indicated that it contains a repertoire of genes which could enable it to survive under different abiotic stress conditions for e.g., elevated mercury concentrations, to interact with plants via root colonization, to produce phytohormones and siderophores, to fix nitrogen and to interact with bacterial signaling via a LuxR-solo and quorum quenching. Based on the identified genes, functional analyses were performed in vitro with RL1 under different growth conditions. The R. qingshengii type strain djl6 and a closely related Rhodococcus erythropolis BG43 were included in the experiments to find common and distinct traits between the strains. Genome based phylogenetic analysis of 15 available and complete R. erythropolis and R. qingshengii genome sequences revealed a separation of the R. erythropolis clade in two subgroups. First one harbors only R. erythropolis strains including the R. erythropolis type strain. The second group consisted of the R. qingshengii type strain and a mix of R. qingshengii and R. erythropolis strains indicating that some strains of the second group should be considered for taxonomic re-assignment. However, BG43 was clearly identified as R. erythropolis and RL1 clearly as R. qingshengii and the strains had most tested traits in common, indicating a close functional overlap of traits between the two species.

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Section: Discussionmentioning

confidence: 99%

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

et al. 2021

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“…It is known that several beneficial microorganisms can promote iron uptake by plants based on the mechanisms of chelation, reduction, acidification and induction, among which the induction of plant systemic resistance mediated by volatile organic compounds (VOCs) has attracted wide attention in recent years (Back et al, 2020). Compared with soluble compounds, the VOCs released by rhizosphere microorganisms have two advantages in the interaction of microbial communities: first, volatile compounds with low molecular weights (<300 Da) can evaporate and migrate freely via soil pores over long distances (Sharifi and Ryu, 2018a,b); second, these gaseous substances act as signal molecules and can activate or enhance plant defense responses (Fincheira and Quiroz, 2018;Netzker et al, 2020). Some studies showed that Bacillus subtilis GB03 directly and indirectly promoted the growth of Arabidopsis thaliana under iron deficiency through the emission of acetoin and 2,3-butanediol (Zhang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Iron Uptake in Plants by Volatile Emissions of Rahnella aquatilis JZ-GX1

Kong

Wang

2021

Front. Plant Sci.

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Iron deficiency in soil has crucially restricted agricultural and forestry production. Volatile organic compounds (VOCs) produced by beneficial microorganisms have been proven to play an important role in inducing abiotic stress tolerance in plants. We investigated the effects of VOCs released by the rhizobacterium Rahnella aquatilis JZ-GX1 on the growth and root parameters of Arabidopsis thaliana under iron deficiency. The effect of the rhizobacterial VOCs on the gene expression in iron uptake and hormone signaling pathways were detected by RT-qPCR. Finally, the VOCs of the JZ-GX1 strain that could promote plant growth under iron deficiency stress were screened. The results showed that the JZ-GX1 strain could induce A. thaliana tolerance to iron deficiency stress by promoting the development of lateral roots and root hairs and increasing the activities of H+ ATPase and Fe3+ reductase. In addition, the AHA2, FRO2, and IRT1 genes of A. thaliana exposed to JZ-GX1-emitted VOCs were upregulated 25-, 1. 81-, and 1.35-fold, respectively, and expression of the abscisic acid (ABA) synthesis gene NCED3 was upregulated on both the 3rd and 5th days. Organic compounds were analyzed in the headspace of JZ-GX1 cultures, 2-undecanone and 3-methyl-1-butanol were found to promote Medicago sativa and A. thaliana growth under iron-limited conditions. These results demonstrated that the VOCs of R. aquatilis JZ-GX1 have good potential in promoting iron absorption in plants.

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“…Many microbial species such as Bacillus spp., Pseudomonas spp., Trichoderma spp., Streptomyces spp., and Acinetobacter spp. have been shown to effectively control FOC ( Raza et al, 2017 ; Kumar et al, 2020 ; Netzker et al, 2020 ). The primary mechanism probably involves secreting antimicrobial compounds, competing for biological niche and nutrients, and inducing plant resistance ( Shafi et al, 2017 ; Netzker et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Biocontrol of Cucumber Fusarium Wilt by Combined Application of New Antagonistic Bacteria Bacillus amyloliquefaciens B2 and Phenolic Acid-Degrading Fungus Pleurotus ostreatus P5

Wang

Cai

et al. 2021

Front. Microbiol.

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Continuous monoculture of cucumber (Cucumis sativus L.) typically leads to the frequent incidence of Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC). As potent allelochemicals, phenolic acids are believed to be associated with soilborne diseases. This study aimed to investigate the effect of single or co-inoculation of antagonistic bacteria Bacillus amyloliquefaciens B2 and phenolic acid-degrading fungus Pleurotus ostreatus P5 on the suppression of cucumber Fusarium wilt. The strain B2 was identified as B. amyloliquefaciens based on biochemical, physiological, and 16S rDNA and gyrB gene sequence analyses. Strain B2 showed indole-3-acetic acid (IAA) and siderophore production and phosphate solubilization in in vitro assays. Scanning electron microscope (SEM) imaging showed the ability of strain B2 to adhere to the root surface of cucumber. P. ostreatus P5 could effectively degrade mixed phenolic acids as its sole source of carbon and energy for growth in liquid medium. In a pot experiment, four treatments were established as follows: (1) CK, uninoculated control; (2) B2, inoculation of strain B2; (3) P5, inoculation of strain P5; and (4) B2 + P5, co-inoculation of strain B2 and strain P5. At the end of the 60-day pot experiment, the B2, P5, and B2 + P5 treatments significantly reduced disease incidence by 48.1, 22.2, and 63.0%, respectively, compared to the CK treatment (p < 0.05). All three inoculation treatments significantly increased the growth of cucumber seedlings and suppressed the FOC population compared to the control (p < 0.05). High-performance liquid chromatography (HPLC) analysis showed that total phenolic acids were decreased by 18.9, 35.9, and 63.2% in the B2, P5, and B2 + P5 treatments, respectively. The results from this study suggest that combined application of B. amyloliquefaciens B2 and P. ostreatus P5 could be a promising strategy for suppressing Fusarium wilt and improving plant growth of cucumber seedlings under continuous cropping conditions.

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Bacterial Volatile Compounds: Functions in Communication, Cooperation, and Competition

Cited by 71 publications

References 101 publications

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

Enhanced Iron Uptake in Plants by Volatile Emissions of Rahnella aquatilis JZ-GX1

Enhanced Biocontrol of Cucumber Fusarium Wilt by Combined Application of New Antagonistic Bacteria Bacillus amyloliquefaciens B2 and Phenolic Acid-Degrading Fungus Pleurotus ostreatus P5

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