Bacterial utilization of algal extracellular products. 1. The kinetic approach

Bell, Wayne H.

doi:10.4319/lo.1980.25.6.1007

Cited by 46 publications

(33 citation statements)

References 18 publications

(15 reference statements)

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“…(n=12) net total (n=4) resp. (n=12) net (n=12) total (n=4) Bell (1980a). Statistics represent &SE for n=15 (resp.…”

Section: Resultsmentioning

confidence: 99%

“…Experimental protocol for kinetic assay of heterotrophic [14C]EP utilization is based on the mixed-substrate models derived by Bell (198Oa) Bell (1980a) showed that a plot of t/f' vs. A' will yield a straight line analogous to that obtained in pure-substrate analyses. IIowever, the kinetic parameters determined from such a line will tend to overestimate the "true" parameters for each substrate if such could be determined by Kinetic experiments were performed according to the basic protocol of Bell and Sakshaug (1980)) with a total sample volume of 10 ml in each 60-ml serum bottle consisting of 5 ml of bacterial suspension and 5 ml of diluted [14C]EP providing a range of A' of about 30-200 rug C-liter-I.…”

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confidence: 99%

“…14C-labeled EP ([14C]EP) were prepared, stored, and cleansed as described by Bell (1980a). Immediately before experimentation, the [lC]EP were diluted in sterilefiltered (0.22 pm) natural or artificial seawater to 300-400 pg Caliter-I .…”

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confidence: 99%

“…Total uptake rate is defined as the sum of respiration plus net uptake rates; %EP respiration is determined separately at each A' as [(respiration dpm)/(total uptake dpm)] X 100%. All samples were corrected with appropriate blanks prepared at t = 0 (Bell 1980a).…”

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confidence: 99%

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Bacterial utilization of algal extracellular products. 3. The specificity of algal‐bacterial interaction

Bell

1983

Limnology & Oceanography

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Kinetic analyses were made of the utilization of '"C-labeled algal extracellular products (EP) by bacterial populations in continuous algal cultures containing steady state Skeletonema costatum or Dunaliella tertiolecta. The development of bacteria adapted to the respective algal species was revealed by gradual evolution of a common X-intercept for net EP uptake and respiration lines from initially disparate estimates. When the adapted populations were given labeled EP prepared from other algal species, linear kinetics were retained but the X-intercepts for net uptake and respiration failed to coincide. Bacterial response to a given algal EP pool does not necessarily preclude enzyme-mediated utilization of organic substrates from other species. Rapid utilization of algal EP appears to be a consequence of bacterial adaptation to existence under dilute nutrient conditions rather than a response to a specific alga. Prolonged exposure to a single alga may favor the development of bacterial populations in which metabolism is limited by enzyme-mediated transport of the compounds available in its EP pool, but this phenomenon is less significant ecologically than the general stimulation of bacterial activity effected by the increased supply of these compounds.

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“…(n=12) net total (n=4) resp. (n=12) net (n=12) total (n=4) Bell (1980a). Statistics represent &SE for n=15 (resp.…”

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Bacterial utilization of algal extracellular products. 3. The specificity of algal‐bacterial interaction

Bell

1983

Limnology & Oceanography

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“…Although the selective filtration methods used in these studies result in ambiguities in production and utilization rates (see critique in Smith and Horner, 1981), bacterial incorporation of label was consistently observed. A variety of attempts have been made to distinguish the production and heterotrophic utilization of photosynthesized dissolved organic matter including kinetic analyses of isotope distribution between dissolved and particulate phases (Wiebe and Smith, 1977;Bell, 1980Bell, , 1983Bell and Sakshaug, 1980;Lt, 1983) and use of antibiotics (e.g. Jensen, 1984).…”

Section: List Of Tables (Continued)mentioning

confidence: 99%

Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter

Lohrenz¹

1985

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Kinetic models for the growth ofEscherichia coli with mixtures of sugars under carbon-limited conditions

Lendenmann

Egli

1998

Biotechnol. Bioeng.

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In natural environments, heterotrophic microorganisms encounter complex mixtures of carbon sources, each of which is present only at very low concentrations. Under such conditions no significant growth could be expected if cells utilized only one of the available carbon compounds as suggested by the principle of diauxic growth. Indeed, there is much evidence that microbial cells utilize many carbon sources simultaneously. In order to predict bacterial growth under such conditions we developed a model describing the specific growth rate as a function of the individual concentrations of several simultaneously utilized carbon substrates. Together with multisubstrate models previously published, this model was evaluated for its ability to describe growth of Escherichia coli during the simultaneous utilization of mixtures of sugars in carbon‐limited continuous culture. Using the μmax and Ks constants determined for single substrate growth with six different sugars, the model was able for most experiments to adequately describe the specific growth rate of the culture, i.e., the experimentally set dilution rate, from the measured concentrations of the individual sugars. The model provides an explanation why bacteria can still grow relatively fast under environmental conditions where the concentrations of carbon substrates are usually extremely low. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:99–107, 1998.

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Bacterial utilization of algal extracellular products. 1. The kinetic approach

Cited by 46 publications

References 18 publications

Bacterial utilization of algal extracellular products. 3. The specificity of algal‐bacterial interaction

Bacterial utilization of algal extracellular products. 3. The specificity of algal‐bacterial interaction

Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter

Kinetic models for the growth ofEscherichia coli with mixtures of sugars under carbon-limited conditions

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