Bacterial Transformation

Effect of potassium phosphate on Bacillus subtilis competence mutants

“…(1984) 157, 152-157). Competence factors have been described in Pneumococcus [4,5] and in B. subtilis [6,7]. This buffer stimulates up to 40-50 times the transformation frequency of FB92 and FB94 strains, while it has an inhibitory effect on the other two mutants and on the wild-type strain.…”

“…Competence factors have been described in Pneumococcus [4,5] and in B. subtilis [6,7].In a previous paper we described the isolation and characterization of four different B. subtilis mutants altered in competence development. (1984) 157, 152-157).…”

“…Competence factors have been described in Pneumococcus [4,5] and in B. subtilis [6,7]. (1984) 157, 152-157).…”

Bacillus

“…A more interested reader is referred to a number of excellent and extensive reviews which delve into the physiology and biochemistry of this process (Dubnau, 1976Venema 1979;Smith and Danner, 1981;Dressler and Potter, 1982).…”

Tn916-mediated plasmid transfer in Bacillus subtilis

“…When double stranded DNA binds to these sites, degradation of a single strand of DNA takes place by the nucleases present near the binding sites while the other DNA strand is taken up into the cell. Once the single strand of DNA is transferred into the cell the single strand is then incorporated into the host genome by the host recombination system (Smith et al, 1981, andStewart, 1989 ;Venema, 1979 ;Michel et al, 1982). Ehrlich, (1978) demonstrated that unrelated plasmid DNA can be taken up by the process of transformation in competent B. subtilis.…”