Bacterial spores as particulate carriers for gene gun delivery of plasmid DNA

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

“…DNA has been extensively employed as a biotechnological tool, with applications in different forms of genetic modification, such as gene therapy and silencing (Fichou and Gersbach 2016), in vaccine development (Aps et al 2016) and as vaccine adjuvants (Rozenfeld et al 2012), and even in the delivery of macromolecules (as hydrogels and DNA cages) (Campolongo et al 2010;Walsh et al 2011). In many of these applications, the DNA molecules used are either in the form of long and circular plasmids (see, for example, Aps et al 2016;Robinson-Hamm and Gersbach 2016) or as small linear oligonucleotides (Fichou and Férec 2006;Rozenfeld et al 2012).…”

“…In many of these applications, the DNA molecules used are either in the form of long and circular plasmids (see, for example, Aps et al 2016;Robinson-Hamm and Gersbach 2016) or as small linear oligonucleotides (Fichou and Férec 2006;Rozenfeld et al 2012). The effect of small single-stranded DNA oligonucleotides on the structure of DODAB vesicles has been studied with ESR spectroscopy (Rozenfeld et al 2015a).…”

“…Versatile DODAB membranes have been used as nucleic acid (Silva et al 2011) and hydrophobic drug carriers Vieira et al 2006), as templates for polymerization and deposition of materials (Hubert et al 2000), as biomimetic particles (Rosa et al 2008), as microbicidal agents (Ragioto et al 2014) and as vaccine adjuvants (Rozenfeld et al 2012;Aps et al 2016). Similarly, highly fusogenic diC14-amidine membranes (Oliveira et al 2012) have successfully delivered nucleic acids (El Ouahabi et al 1996;Ruysschaert et al 1994) and stimulated the formation of immune responses (Tanaka et al 2008).…”

The Versatile Dioctadecyldimethylammonium Bromide

“…DODAB was also used to treat spores of Bacillus subtilis aiming at gene gun delivery of DNA plasmids in mice; DODAB treated spores allowed efficient plasmid adsorption and could be loaded into biolistic cartridges and efficiently delivered into mice for induction of specific cellular and antibody responses required for DNA vaccines in vivo [93].…”

Peptide‐based supramolecular hydrogels for delivery of biologics

“…For example, the therapeutic efficiency of DNAs that encode for antigenic protein may be limited by rapid enzymatic degradation and fragmentation of DNA during the long circulation time and the poor cell permeability that results from the highly negatively charged phosphate backbone. Viral vehicles and non‐viral delivery systems such as gene gun, nanoparticles, and liposomes have been widely explored . However, these delivery systems may be accompanied by other disadvantages such as toxicity and impairment of DNA activity.…”