“…Thus, by keeping a high concentration of QS signals and minimising the impact of QQ, the efficacy of the METs can be improved ( Fig. 2 ), for example, by preserving the stability of aerobic granules for stable wastewater treatment [ 25 ]. Fig.…”
Section: Importance Of Qs/qq Assistance In the Application Of Metsmentioning
“…Thus, by keeping a high concentration of QS signals and minimising the impact of QQ, the efficacy of the METs can be improved ( Fig. 2 ), for example, by preserving the stability of aerobic granules for stable wastewater treatment [ 25 ]. Fig.…”
Section: Importance Of Qs/qq Assistance In the Application Of Metsmentioning
“…These were substantially slower rates compared to when E. coli TOP10-AiiO or Rhodococcus sp. BH4 was tested in suspension, both of which completely degraded OHHL within several minutes [19,25]. This is because the rate at which AHL diffused into the beads' interior affected the overall degradation rate.…”
Section: Effect Of Qq Beads On Biofilm Formationmentioning
confidence: 99%
“…Disrupting bacterial QS, known as quorum quenching (QQ), can be an effective method to inhibit biofilm formation. In particular, extensive research on controlling biofouling in membrane processes using QQ bacteria that disrupt the QS process of AHLs has been carried out in the past decade [19,20]. Specifically, the technique of entrapping QQ bacteria within hydrogel beads has gained popularity as a means of implementing the QQ strategy in continuous water treatment processes [15,21].…”
Membrane biofouling is an inevitable challenge in membrane-based water treatment systems such as membrane bioreactors. Recent studies have shown that biological approaches based on bacterial signaling can effectively control biofilm formation. Quorum quenching (QQ) is known to inhibit biofilm growth by disrupting quorum sensing (QS) signaling, while nitric oxide (NO) signaling helps to disperse biofilms. In this study, batch biofilm experiments were conducted to investigate the impact of simultaneously applying NO signaling and QQ for biofilm control using Pseudomonas aeruginosa PAO1 as a model microorganism. The NO treatment involved the injection of NONOates (NO donor compounds) into mature biofilms, while QQ was implemented by immobilizing QQ bacteria (Escherichia coli TOP10-AiiO or Rhodococcus sp. BH4) in alginate or polyvinyl alcohol/alginate beads to preserve the QQ activity. When QQ beads were applied together with (Z)-1-[N-(3-aminopropyl)-N-(n-propyl) amino]diazen-1-ium-1,2-diolate (PAPA NONOate), they achieved a 39.0% to 71.3% reduction in biofilm formation, which was substantially higher compared to their individual applications (16.0% to 54.4%). These findings highlight the significant potential of combining QQ and NO technologies for effective biofilm control across a variety of processes that require enhanced biofilm inhibition.
“…These are bio-electrochemical systems that use biofilms as catalysts to convert chemical energy into electrical energy. They can generate electricity through low-grade biomass and even through wastewater [ 207 ]. They have a cathode chamber, an anaerobic anode chamber, a salt bridge, or a proton exchange membrane (PEM) that works as a separating unit between both chambers and only allow protons (H + ) to pass to cathode from anode.…”
Section: Potential Applications Of Qs Regulated Biofilms For Wwtsmentioning
confidence: 99%
“…Other than controlling rhlI, LasR, a transcriptional regulator, regulates the pqsABCDE operon positively to produce PQS, another QS signal for P. aeruginosa . In contrast to this, phz, which controls the production of phenazine and helps in electron transfer to the anode, is controlled by pqs operon [ 207 ]. In a study, for QS overexpression, multi-copy broad-host plasmid pYC-rhlIR was transformed in strain CGMCC 1.860.…”
Section: Potential Applications Of Qs Regulated Biofilms For Wwtsmentioning
Quorum sensing (QS), a type of bacterial cell–cell communication, produces autoinducers which help in biofilm formation in response to cell population density. In this review, biofilm formation, the role of QS in biofilm formation and development with reference to biological wastewater treatment are discussed. Autoinducers, for example, acyl-homoserine lactones (AHLs), auto-inducing oligo-peptides (AIPs) and autoinducer 2, present in both Gram-negative and Gram-positive bacteria, with their mechanism, are also explained. Over the years, wastewater treatment (WWT) by QS-regulated biofilms and their optimization for WWT have gained much attention. This article gives a comprehensive review of QS regulation methods, QS enrichment methods and QS inhibition methods in biological waste treatment systems. Typical QS enrichment methods comprise adding QS molecules, adding QS accelerants and cultivating QS bacteria, while typical QS inhibition methods consist of additions of quorum quenching (QQ) bacteria, QS-degrading enzymes, QS-degrading oxidants, and QS inhibitors. Potential applications of QS regulated biofilms for WWT have also been summarized. At last, the knowledge gaps present in current researches are analyzed, and future study requirements are proposed.
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