“…The proteins that are to be exported outside the cell contain N-terminal leader peptide sequences, which are key determinants for particular secretory pathways [ 8 , 9 , 10 ]. Gram-positive bacteria, including S. aureus , have at least six different secretion pathways, consisting of the general secretory pathway (Sec-type), the twin-arginine (Tat) pathway, the pseudopilin-like (Com) pathway, ESAT-6, bacteriocin, and the Holins leader peptide pathway [ 11 , 12 , 13 , 14 ].…”