Bacterial sensing of and resistance to gold salts

Checa, Susana K.; Espariz, Martín; Audero, María E. Pérez; Botta, Pablo E.; Spinelli, Silvana Virginia; Soncini, Fernando C.

doi:10.1111/j.1365-2958.2007.05590.x

Cited by 118 publications

(213 citation statements)

References 54 publications

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“…Addition of 1 mM CuSO 4 to the culture medium induced copA expression by 13-fold in LB (Fig. 1b) and by eightfold in SM9 minimal medium (Checa et al, 2007). This copperdependent activation of copA was eliminated in a cueR null mutant, but not affected in a DgolS strain, confirming that CueR controls the expression of the Cu(I) transporter CopA in Salmonella.…”

Section: Expression Of Copa In Salmonella Is Directly Controlled By Cuermentioning

confidence: 62%

“…We have recently shown that Salmonella has a second CueR homologue highly sensitive to gold ions, GolS, which induces the expression of a CopA-homologous protein, GolT, and a putative metal-binding protein, GolB (Checa et al, 2007). We found that this Salmonella-specific regulon is required for gold resistance, but not for copper tolerance, except in a strain in which the main copper transporter CopA has been deleted (Checa et al, 2007; see also Table 3).…”

Section: Copa and Cuid Are Essential For Copper Tolerance Under Both mentioning

confidence: 79%

“…Bacterial strains (all derivatives of S. Typhimurium 14028s) used in this study are listed in Table 1. Bacterial strains were grown overnight at 37 uC in Luria broth (LB) or LB-agar plates (Checa et al, 2007). Ampicillin, kanamycin and chloramphenicol were used at 100, 25 and 10 mg ml 21 , respectively.…”

Section: Methodsmentioning

confidence: 99%

“…After incubation, c.f.u. per ml were calculated and the percentage survival was estimated based on the count of the corresponding strain grown in the absence of metal added (Checa et al, 2007).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, S. Typhimurium harbours a second cue-like regulon, gol. We have recently shown that this regulon, which includes a Ptype ATPase (GolT), a putative metal-binding protein (GolB) and a transcriptional regulator (GolS), endows S. Typhimurium with resistance to gold salts (Checa et al, 2007). The golTS operon is present in most Salmonella subspecies and in Salmonella bongori (http://www.sanger.…”

Section: Typhi and S Typhimurium Copper-response Regulatory Networkmentioning

confidence: 99%

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Dissecting the Salmonella response to copper

et al. 2007

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Section: Expression Of Copa In Salmonella Is Directly Controlled By Cuermentioning

confidence: 62%

Section: Copa and Cuid Are Essential For Copper Tolerance Under Both mentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Typhi and S Typhimurium Copper-response Regulatory Networkmentioning

confidence: 99%

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Dissecting the Salmonella response to copper

et al. 2007

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Copper(I) ATPases: Transport Mechanism and Cellular Functions in Bacteria

Argüello

Padilla‐Benavides

Collins

2004

Encyclopedia of Inorganic and Bioinorganic Chemistry

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Transmembrane transporters are basic components of homeostatic systems that determine the cellular distribution of transition‐metal ions. Among these, P 1B1 ‐type ATPases mediate cytoplasmic Cu(I) efflux. Prokaryotic Cu(I)‐ATPases are powerful tools to study the mechanism of transport and metal homeostasis in cells. They are similar to the eukaryotic homologs that play various unique roles in simple bacterial systems. Structural and functional characterizations of bacterial Cu(I)‐ATPases have shown the transport stoichiometry, metal coordination during transport, regulation by cytoplasmic metal‐sensing domains, and the mechanism of chaperone‐mediated Cu(I) access to transport sites. The high resolution structure of Legionella pneumophila Cu(I)‐ATPase has revealed how the canonical structure of P‐type ATPases has adapted to enable Cu(I)‐transfer and translocation through a mechanism similar to the classical Albers–Post model. In addition, the importance of bacterial Cu(I)‐ATPases to the assembly of periplasmic and secreted metalloproteins is now clear, suggesting the participation of periplasmic Cu(I)‐trafficking proteins, which not only buffer the metal but also direct Cu(I) to the correct target apoproteins. Here, we discuss how the attributes of Cu(I)‐ATPases contribute to novel copper transport and homeostasis models.

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Metal Specificity of Metallosensors

Higgins

Giedroc

2004

Encyclopedia of Inorganic and Bioinorganic Chemistry

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Transition metal ions are biologically required yet toxic when in excess. As such, prokaryotes have developed metal homeostasis systems that allow for the acquisition of essential metal ions, the delivery of these metal ions to target proteins, and the export of metal ions from the cell. Specialized metal‐binding proteins termed metallosensors bind to a cognate metal ion(s) in the cell and either transcriptionally repress the expression of importers or de‐repress the expression of exporters or sequestration systems and therefore govern cellular metal homeostasis. Metallosensors must be selective and appropriately sensitive toward the binding of their cognate metals. Prokaryotic metallosensors have been identified in ten different protein structural families. Each family exploits either one general metal‐binding‐site region, individual members of which have evolved different coordination sites, or alternatively, employs multiple metal‐binding sites to effect coordination of different metal ions. There is now broad support for the hypothesis that metal responsiveness in metallosensors is most closely linked to the coordination number and geometry adopted by cognate metal ion(s), and this is the subject of this article. Since a range of metal ions can be collectively sensed by a single repressor family, a particular protein fold cannot be specific for one particular metal ion. In fact, the converse is true: nature has used convergent evolution to evolve metal‐specific sensors on a variety of structural scaffolds that exploit common principles of coordination chemistry (ligand type, coordination number, and geometry) to effect the same biological outcome.

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Bacterial sensing of and resistance to gold salts

Cited by 118 publications

References 54 publications

Dissecting the Salmonella response to copper

Dissecting the Salmonella response to copper

Copper(I) ATPases: Transport Mechanism and Cellular Functions in Bacteria

Metal Specificity of Metallosensors

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