Bacterial responses to environmental change on the <scp>T</scp>ibetan <scp>P</scp>lateau over the past half century

Liu, Yongqin; Priscu, John C.; Yao, Tandong; Vick‐Majors, Trista J.; Xu, Bin; Jiao, Nianzhi; Santibáñez, Pamela A.; Huang, Sijun; Wang, Ninglian; Greenwood, Mark C.; Michaud, Alexander B.; Kang, Shichang; Wang, Jianjun; Gao, Qun; Yang, Yunfeng

doi:10.1111/1462-2920.13115

Cited by 24 publications

(37 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This data confirmed a temporal dependence of microbial cell density in Scarisoara cave perennial ice. Similar tendency of total cell counts and live/dead ratio has been previously observed for the permafrost microcosms formed during the last decade 35 , and 27 kyr to 33 kys BP 36 . The viable cell density of each ice sample (Table 1 ), calculated as a difference between the total and dead cell contents, showed comparable values in sediment-rich ice strata 1-S (4.4 × 10 5 cells mL −1 ) and 400-O (3.4 × 10 5 cells mL −1 ), and 5-fold lower in older ice 900-O (0.8 × 10 5 cells mL −1 ).…”

Section: Resultssupporting

confidence: 85%

“…Therefore, ice layers formed during the dryer LIA period (sample 400-O) showed a higher (by 3.5-fold) microbial cell density relative to that of the wetter MWP period (sample 900-O), in accordance with differences in the precipitation regime. Such correlation was also observed in the glacier ice cores from Tibetan Plateau, where a higher microbial abundance was reported for dryer regions, while lower abundance occurred in areas with high precipitations affected by the Indian Monsoon 35 . Also, Antarctic ice core formed during the last 68 kyr showed higher cell density in strata formed during the colder and dryer Last Glacial Maximum period 68 .…”

Section: Discussionsupporting

confidence: 70%

See 1 more Smart Citation

Bacterial and archaeal community structures in perennial cave ice

Iţcuş¹,

Pascu²,

Lavín

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Ice entrenched microcosm represents a vast reservoir of novel species and a proxy for past climate reconstitution. Among glacial ecosystems, ice caves represent one of the scarcely investigated frozen habitats. To characterize the microbial diversity of perennial ice from karst ecosystems, Roche 454 sequencing of 16S rRNA gene amplicons from the underground ice block of Scarisoara Ice Cave (Romania) was applied. The temporal distribution of bacterial and archaeal community structures from newly formed, 400, and 900 years old ice layers was surveyed and analyzed in relation with the age and geochemical composition of the ice substrate. The microbial content of cave ice layers varied from 3.3 104 up to 7.5 105 cells mL−1, with 59–78% viability. Pyrosequencing generated 273,102 reads for the five triplicate ice samples, which corresponded to 3,464 operational taxonomic units (OTUs). The distribution of the bacterial phyla in the perennial cave ice varied with age, organic content, and light exposure. Proteobacteria dominated the 1 and 900 years old organic rich ice deposits, while Actinobacteria was mostly found in 900 years old ice strata, and Firmicutes was best represented in 400 years old ice. Cyanobacteria and Chlorobi representatives were identified mainly from the ice block surface samples exposed to sunlight. Archaea was observed only in older ice strata, with a high incidence of Crenarchaeota and Thaumarchaeaota in the 400 years old ice, while Euryarchaeota dominated the 900 years old ice layers, with Methanomicrobia representing the predominant taxa. A large percentage (55.7%) of 16S rRNA gene amplicons corresponded to unidentified OTUs at genus or higher taxa levels, suggesting a greater undiscovered bacterial diversity in this glacial underground habitat. The prokaryotes distribution across the cave ice block revealed the presence of 99 phylotypes specific for different ice layers, in addition to the shared microbial community. Ice geochemistry represented an important factor that explained the microbial taxa distribution in the cave ice block, while the total organic carbon content had a direct impact on the cell density of the ice microcosm. Both bacterial and archaeal community structures appeared to be affected by climate variations during the ice formation, highlighting the cave ice microbiome as a source of putative paleoclimatic biomarkers. This report constitutes the first high-throughput sequencing study of the cave ice microbiome and its distribution across the perennial underground glacier of an alpine ice cave.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Discussionsupporting

confidence: 70%

Bacterial and archaeal community structures in perennial cave ice

Iţcuş¹,

Pascu²,

Lavín

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This might be largely due to the differences in dispersal ability, habitat availabilities, or home range requirements among the various taxonomic groups. For instance, birds have on average larger geographic ranges than do mammals (Gaston, ), while microbes have larger elevational range size than macroinvertebrates, perhaps due to dispersal via air and across large distances over continents (Liu et al ., ; Wang et al ., ). Thus, for the first time, we have highlighted significant differences in NBRS relationships between micro‐ and macroorganisms; such comparisons had been missing from previous studies because microorganisms have simply been less considered overall (Gaston, ; Slatyer et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Thermal barriers constrain microbial elevational range size via climate variability

Wang

Soininen

2017

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Range size is invariably limited and understanding range size variation is an important objective in ecology. However, microbial range size across geographical gradients remains understudied, especially on mountainsides. Here, the patterns of range size of stream microbes (i.e., bacteria and diatoms) and macroorganisms (i.e., macroinvertebrates) along elevational gradients in Asia and Europe were examined. In bacteria, elevational range size showed non-significant phylogenetic signals. In all taxa, there was a positive relationship between niche breadth and species elevational range size, driven by local environmental and climatic variables. No taxa followed the elevational Rapoport's rule. Climate variability explained the most variation in microbial mean elevational range size, whereas local environmental variables were more important for macroinvertebrates. Seasonal and annual climate variation showed negative effects, while daily climate variation had positive effects on community mean elevational range size for all taxa. The negative correlation between range size and species richness suggests that understanding the drivers of range is key for revealing the processes underlying diversity. The results advance the understanding of microbial species thermal barriers by revealing the importance of seasonal and diurnal climate variation, and highlight that aquatic and terrestrial biota may differ in their response to short- and long-term climate variability.

show abstract

“…The morphology and specific climatic conditions of these caves allowed the accumulation of perennial underground ice deposits constituting paleoclimate archives (Perșoiu and Lauritzen, 2018). Therefore, the ice-entrapped microorganisms could be used as proxies for climate variation studies, while the response of the microbiome composition and abundance to changes in temperature and precipitation regimes during ice formation is expected to reflect the impact of climatic variations on ice depositional processes (Liu and others, 2016).…”

Section: Introductionmentioning

confidence: 99%

High-throughput sequencing of fungal communities across the perennial ice block of Scărișoara Ice Cave

Mondini¹,

Donhauser

Iţcuş³

et al. 2018

Ann. Glaciol.

View full text Add to dashboard Cite

This survey presents the first high-throughput characterisation of fungal distribution based on ITS2 Illumina sequencing of uncultured microbiome from a 1500 years old perennial ice deposit in Scărișoara Ice Cave, Romania. Of the total of 1 751 957 ITS2 sequences, 64% corresponded to 182 fungal operational taxonomic units, showing a low diversity, particularly in older ice strata, and a distinct temporal distribution pattern. Ascomycota was the major phylum in all ice samples, dominating the 400 and 1500 years old ice strata deposited during the cold Little Ice Age (LIA) and Dark Ages Cold Period, while Basidiomycota was mostly present in 900-years old ice formed during the Medieval Warm Period (MWP). Chytridiomycota and Mucoromycota phyla were present in recently formed and 400-years old ice, respectively. Among the 80 identified genera, Cryptococcus victoriae, commonly found in glacial habitats, was identified in all strata. A positive correlation between fungal distribution and ice conductivity, Ca, Na and Sr concentrations was observed across the ice block, with pH values trailing climate variations during LIA and MWP, respectively. Our record highlighted the presence of a complex climate and environmental-driven fungal community in perennial ice strata accumulated during the last 1500 years in Scărișoara Ice Cave.

show abstract

Bacterial responses to environmental change on the Tibetan Plateau over the past half century

Cited by 24 publications

References 69 publications

Bacterial and archaeal community structures in perennial cave ice

Bacterial and archaeal community structures in perennial cave ice

Thermal barriers constrain microbial elevational range size via climate variability

High-throughput sequencing of fungal communities across the perennial ice block of Scărișoara Ice Cave

Contact Info

Product

Resources

About