Bacterial resistance in AtNPR1 transgenic sweet orange is mediated by priming and involves EDS1 and PR2

Boscariol-Camargo, Raquel L.; Takita, Marco Aurélio; Machado, Marcos Antônio

doi:10.1007/s40858-016-0108-2

Cited by 25 publications

(19 citation statements)

References 56 publications

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“…Basal levels of SA were not altered in any of the tested lines, and the SAR marker gene in citrus, PR2 , was not constitutively expressed, suggesting that AtNPR1 ectopic expression did not induce a constitutive defense response. A similar effect was shown in AtNPR1 -overexpressing sweet orange lines by Boscariol-Camargo et al 48 . The most conspicuous effect was an increase in the accumulation of enhanced disease susceptibility1 ( EDS1 ), PR1 and PR2 transcripts 12–24 h after inoculation by Xcc .…”

Section: Use Of Atnpr1 and Its Orthologs For Crop supporting

confidence: 83%

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NPR1 as a transgenic crop protection strategy in horticultural species

et al. 2018

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The NPR1 (NONEXPRESSOR OF PATHOGENESIS RELATED GENES1) gene has a central role in the long-lasting, broad-spectrum defense response known as systemic acquired resistance (SAR). When overexpressed in a transgenic context in Arabidopsis thaliana, this gene enhances resistance to a number of biotic and abiotic stresses. Its position as a key regulator of defense across diverse plant species makes NPR1 a strong candidate gene for genetic engineering disease and stress tolerance into other crops. High-value horticultural crops face many new challenges from pests and pathogens, and their emergence exceeds the pace of traditional breeding, making the application of NPR1-based strategies potentially useful in fruit and vegetable crops. However, plants overexpressing NPR1 occasionally present detrimental morphological traits that make its application less attractive. The practical utility of NPR-based approaches will be a balance of resistance gains versus other losses. In this review, we summarize the progress on the understanding of NPR1-centered applications in horticultural and other crop plants. We also discuss the effect of the ectopic expression of the A. thaliana NPR1 gene and its orthologs in crop plants and outline the future challenges of using NPR1 in agricultural applications.

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Section: Use Of Atnpr1 and Its Orthologs For Crop supporting

confidence: 83%

“…citri .) Citrus greening (Huanglongbing) a Zhang et al 9 Dutt et al 50 Boscariol-Camargo et al 48 Cotton Verticillium wilt ( V . dahliae ) Fusarium wilt ( F .…”

Section: Use Of Atnpr1 and Its Orthologs For Crop mentioning

confidence: 99%

NPR1 as a transgenic crop protection strategy in horticultural species

et al. 2018

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“…Ali et al reported that Brassica juncea transgenic plants overexpressing BjNPR1 exhibited broad spectrum of disease resistance not only to necrotrophic pathogens but also to biotrophic pathogens (Ali et al 2017). Additionally, sweet orange plants overexpressing MhNPR1 confers resistance to Xanthomonas citri as well as induces battery of pathogen related genes (Boscariol-Camargo et al 2016). In monocots, researches have revealed that ectopic expressing NPR1 gene in rice and wheat crops confers broad spectrum of disease resistance against most disastrous pathogens Rhizoctonia solani, Fusarium graminearum, and Fusarium oxysporum, respectively (Molla et al 2016;Quilis et al 2008;Yu et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a <i>Mimulus lewisii</i> NPR1 gene involved in regulating plant resistance to <i>Rhizoctonia solani</i>

Shi

2018

Plant Biotechnology

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The monkey flower Mimulus lewisii is a new emerging model plant for the study in corolla tube formation, pigmentation patterns and pollinator selection, etc. However, the cultivation and management of this plant are difficult due to its susceptibility to a wide range of pathogens and the lack of rigid varieties with high levels of resistance to pathogens. In this regard, genetic engineering is a promising tool that may possibly allow us to enhance the M. lewisii disease resistance against pathogens. Here, we reported the isolation and characterization of non-expressor of pathogenesis related gene 1 (NPR1) gene from M. lewisii. The phylogenetic tree constructed based on the deduced sequence of MlNPR1 with homologs from other species revealed that MlNPR1 grouped together with other known NPR1 proteins of Scrophulariaceae family, and was nearest to Mimulus guttatus. Furthermore, expression analysis showed that MlNPR1 was upregulated after SA treatment and fungal infection. To understand the defensive role of this gene, we overexpressed MlNPR1 in M. lewisii. The transgenic lines showed slight phenotypic abnormalities, but constitutive expression of MlNPR1 activates defense signaling pathways by priming the expression of antifungal PR genes. Moreover, MlNPR1 transgenic lines showed enhanced resistance to Rhizoctonia solani there was delay in symptoms and reduced disease severity than non-transgenic plants. Altogether, the present study suggests that increasing the expression level of MlNPR1 may be a promising approach for development of monkey flower cultivars with enhanced resistance to diseases.

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“…Examples of transgenes or genes whose overexpression confer enhanced resistance are shown in Table 4 [124][125][126][127][128][129][130][131][132][133][134][135][136][137][138]. Nonetheless, a main limitation of the transgenic approach is that adoption of genetically modified plants takes many years because of the rigorous field testing required and the long regulation process.…”

Section: Plant Genetic Resistancementioning

confidence: 99%

Bacterial Citrus Diseases: Major Threats and Recent Progress

Jl¹

2017

JBMOA

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Citriculture is an important, highly organized, and competitive sector of the Brazilian economy. Nonetheless, citrus production is constantly threatened by pathogens that cause considerable economic losses and severe social impacts. Among the major citrus diseases are citrus canker (CCK), citrus variegated chlorosis (CVC), and Huanglongbing (HLB), caused by members of the bacterial species Xanthomonas citri (Xcc), Xylella fastidiosa (Xfa), and 'Candidatus Liberibacter' (CaL), respectively. During the last years, management practices for CVC and HLB in Brazil have provided good results, maintaining the disease incidence at low levels. In contrast, CCK re-emerged as the main citrus disease because of the inefficacy of the current eradication program. In this review, we discuss about the biology of the plant-pathogen interactions, several controversial aspects on the taxonomy of the causal agents, the molecular mechanisms they use to cause disease in citrus plants, the strategies used for disease management and their limitations, and some emerging control alternatives that may be available for commercial production in the future, including transgenic and genome-edited plants with enhanced resistance.

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Bacterial resistance in AtNPR1 transgenic sweet orange is mediated by priming and involves EDS1 and PR2

Cited by 25 publications

References 56 publications

NPR1 as a transgenic crop protection strategy in horticultural species

NPR1 as a transgenic crop protection strategy in horticultural species

Cloning and characterization of a <i>Mimulus lewisii</i> NPR1 gene involved in regulating plant resistance to <i>Rhizoctonia solani</i>

Bacterial Citrus Diseases: Major Threats and Recent Progress

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