Bacterial Reduction of Hexavalent Chromium: Kinetic Aspects of Chromate Reduction by<i>Enterobacter cloacae</i>HO1

Ohtake, Hisao; Fujii, Eijiro; Toko, Kiyoshi

doi:10.3109/10242429008992094

Cited by 27 publications

(16 citation statements)

References 9 publications

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“…Nitrate and nitrite concentrations were analysed by ion chromatography using a Dionex 100 (AS4A-SC column/AG4A-SC column) with UV detection at 215 nm (NO 3 À /NO 2 À ) [22]. Cell numbers were counted by direct microscopy in a counting chamber [34].…”

Section: Reduction Experimentsmentioning

confidence: 99%

Anaerobic co-reduction of chromate and nitrate by bacterial cultures of Staphylococcus epidermidis L-02

Vatsouria

Vainshtein

Kuschk

et al. 2005

J IND MICROBIOL BIOTECHNOL

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Industrial wastewater is often polluted by Cr(VI) compounds, presenting a serious environmental problem. This study addresses the removal of toxic, mutagenic Cr(VI) by means of microbial reduction to Cr(III), which can then be precipitated as oxides or hydroxides and extracted from the aquatic system. A strain of Staphylococcus epidermidis L-02 was isolated from a bacterial consortium used for the remediation of a chromate-contaminated constructed wetland system. This strain reduced Cr(VI) by using pyruvate as an electron donor under anaerobic conditions. The aims of the present study were to investigate the specific rate of Cr(VI) reduction by the strain L-02, the effects of chromate and nitrate (available as electron acceptors) on the strain, and the interference of chromate and nitrate reduction processes. The presence of Cr(VI) decreased the growth rate of the bacterium. Chromate and nitrate reduction did not occur under sterile conditions but was observed during tests with the strain L-02. The presence of nitrate increased both the specific Cr(VI) reduction rate and the cell number. Under denitrifying conditions, Cr(VI) reduction was not inhibited by nitrite, which was produced during nitrate reduction. The average specific rate of chromate reduction reached 4.4 micromol Cr 10(10 )cells(-1 )h(-1), but was only 2.0 micromol Cr 10(10 )cells(-1 )h(-1) at 20 degrees C. The maximum specific rate was as high as 8.8-9.8 micromol Cr 10(10 )cells(-1 )h(-1). The role of nitrate in chromate reduction is discussed.

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Section: Reduction Experimentsmentioning

confidence: 99%

Anaerobic co-reduction of chromate and nitrate by bacterial cultures of Staphylococcus epidermidis L-02

Vatsouria

Vainshtein

Kuschk

et al. 2005

J IND MICROBIOL BIOTECHNOL

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“…Different schemes of including inhibition in the growth kinetics model exist in the literature (e.g. Edwards 1970;Luong 1987;Ohtake et al 1990;Yamamoto et al 1993;Christ and Abriola 2007;Jia et al 2007). Andrews ' (1968) equation (4) is the most commonly used form of growth kinetic model that includes inhibition (e.g.…”

Section: Governing Equationsmentioning

confidence: 99%

Dimensionless parameters to summarize the influence of microbial growth and inhibition on the bioremediation of groundwater contaminants

Mohamed

Hatfield

2010

Biodegradation

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Monod expressions are preferred over zero- and first-order decay expressions in modeling contaminants biotransformation in groundwater because they better represent complex conditions. However, the wide-range of values reported for Monod parameters suggests each case-study is unique. Such uniqueness restricts the usefulness of modeling, complicates an interpretation of natural attenuation and limits the utility of a bioattenuation assessment to a small number of similar cases. In this paper, four Monod-based dimensionless parameters are developed that summarize the effects of microbial growth and inhibition on groundwater contaminants. The four parameters represent the normalized effective microbial growth rate (η), the normalized critical contaminant/substrate concentration (S*), the critical contaminant/substrate inhibition factor (N), and the bioremediation efficacy (η*). These parameters enable contaminated site managers to assess natural attenuation or augmented bioremediation at multiple sites and then draw comparisons between disparate remediation activities, sites and target contaminants. Simulations results are presented that reveal the sensitivity of these dimensionless parameters to Monod parameters and varying electron donor/acceptor loads. These simulations also show the efficacy of attenuation (η*) varying over space and time. Results suggest electron donor/acceptor amendments maintained at relative concentrations S* between 0.5 and 1.5 produce the highest remediation efficiencies. Implementation of the developed parameters in a case study proves their usefulness.

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“…The pH needs to be maintained in neutral conditions (7 -7.5) in order to stabilize a biological reduction process above 80 % efficiency. Ohtake et al [23] reported the formation of intermediates that can increase the pH to alkaline conditions. Ohtake et al [23] reported the formation of intermediates that can increase the pH to alkaline conditions.…”

Section: Effects Of Ph Variationmentioning

confidence: 99%

Chromate Reduction in Anaerobic Systems by Bacterial Strain Pseudomonas aeruginosa CRM100

Salamanca¹,

Strunk²,

Engesser³

2013

Chemie Ingenieur Technik

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The biological reduction of soluble chromate(VI) compounds by bacteria was investigated as a possible treatment for industrial wastewater. A chromate(VI) reducing bacterial strain CRM100 was isolated from a wastewater treatment plant and identified as Pseudomonas aeruginosa. Strain CRM100 using citrate as carbon source, was able to reduce 99.8 % of chromate(VI) added at a concentration of 100 mg L -1 . The results demonstrated the potential applicability of CRM100 for the bioremediation of heavy metal contaminated wastewater. Figure 5. Inhibition of sulfate, nitrate and chromate reduction. The initial concentration of sulfate, nitrate and chromate was 100 mg L -1 . 350 mg L -1 perchlorate and 250 mg L -1 tungstate inhibit the nitrate and sulfate reduction, respectively. The growth of CRM100 is inhibit by concentrations of 1000 mg L -1 perchlorate and 1500 mg L -1 tungstate.

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Bacterial Reduction of Hexavalent Chromium: Kinetic Aspects of Chromate Reduction byEnterobacter cloacaeHO1

Cited by 27 publications

References 9 publications

Anaerobic co-reduction of chromate and nitrate by bacterial cultures of Staphylococcus epidermidis L-02

Anaerobic co-reduction of chromate and nitrate by bacterial cultures of Staphylococcus epidermidis L-02

Dimensionless parameters to summarize the influence of microbial growth and inhibition on the bioremediation of groundwater contaminants

Chromate Reduction in Anaerobic Systems by Bacterial Strain Pseudomonas aeruginosa CRM100

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