This research was carried out to assess Diode Laser(DL)therapeutic effects on chronic periodontitis, by reducing pockets depth and minimizing Microbial Counts (MCs (400, 320, 250, 170 and 90) and (410, 350, 300, 260 and 190) for SG and CG respectively, that were confirmed SG were significantly better than CG. DL irradiation revealed anti-microbial effect and reduction of inflammation in periodontal pockets, also, combination with scaling, supports healing of periodontal pockets through microbial eliminating.