Bacterial pyridine hydroxylation is ubiquitous in environment

Sun, Junsong; Xu, Liang; Tang, Yue‐Qin; Chen, Fuming; Zhao, Jianrong; Wu, Xiao‐Lei

doi:10.1007/s00253-013-4818-9

Cited by 20 publications

(17 citation statements)

References 28 publications

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“…Considering phenol and pyridine are representative components in coking wastewater [12,23,27], functions of strains isolated from the coking wastewater, including strain J5-3 T , in degrading phenol and pyridine were investigated in our previous study [28]. Results revealed that strain J5-3 T was unable to use either of the two compounds for growth, and was absent of phenol hydroxylase gene.…”

Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 98%

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

et al. 2014

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A Gram staining negative, rod-shaped, aerobic bacterial strain J5-3(T) with a single polar flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was motile and capable of optimal growth at pH 6-8, 30 °C, and 0-2 % (w/v) NaCl. Its predominant fatty acids were 11-methyl C18:1 ω7c (29.2 %), C16:0 (20.6 %), C19:0 cyclo ω8c (18.2 %), C18:0 (11.0 %), and C18:1 ω7c/C18:1 ω6c (10.9 %) when grown on trypticase soy agar. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids (GL1, GL2), and two unknown phospholipid (PL1, PL2). The predominant ubiquinone was Q-10, and the genome DNA G+C content was 61.7 mol %. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain J5-3(T) belonged to the family Hyphomicrobiaceae in Alphaproteobacteria. It shared the 16S rRNA gene sequence similarities of 93.8-96.1 % with the genus Devosia, 94.5-94.8 % with the genus Pelagibacterium, and <92.0 % with all the other type strains in family Hyphomicrobiaceae. It can be distinguished from the closest phylogenetic neighbors based on several phenotypic and genotypic features, including α-galactosidase activity, tetracycline susceptibility, major fatty acid composition, polar lipid profile, DNA gyrase B subunit (gyrB) gene sequence, and random-amplified polymorphic DNA profile. Therefore, we consider strain J5-3(T) to represent a novel species of a novel genus within the family Hyphomicrobiaceae, for which the name Paradevosia shaoguanensis gen. nov., sp. nov. is proposed. The type strain of Paradevosia shaoguanensis is J5-3(T) (=CGMCC 1.12430(T) =LMG 27409(T)).

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Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 98%

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

et al. 2014

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“…indole, pyridine and quinoline) usually co‐exist in petrochemical and coking wastewater . Previous studies indicated that the phenol‐degrading strains showed potential for biodegradation and biotransformation of N ‐heterocyclic compounds, such as pyridine, quinoline, indole and carbazole . In this study, it was proven that the nitrogen‐containing organic pollutants (indole, pyridine and quinoline) could be effectively removed by phenol‐stimulated activated sludge.…”

Section: Discussionmentioning

confidence: 71%

“…QM and phenol‐stimulated activated sludge could catalyze the biotransformation of indole to indigoids . Several phenol‐degrading strains, Diaphorobacter , Acidovorax , Acinetobacter and Corynebacterium , were able to transform pyridine via hydroxylation by phenol hydroxylase . The addition of phenol‐degrading microorganism ( Lysinibacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of nitrogen‐containing organic pollutants using phenol‐stimulated activated sludge: performance and microbial community analysis

Zhang

You

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Coking wastewater generally contains high levels of phenolics and nitrogen‐containing organic pollutants, thus seeking an appropriate wastewater treatment strategy is necessary. In this study, the triplicate phenol‐stimulated activated sludges (group C) were constructed for aerobic treatment of nitrogen‐containing organic pollutants (indole, pyridine and quinoline), and glucose‐stimulated sludges (group B) were used as control. RESULTS Both groups maintained high efficiency (>90%) for removal of nitrogen‐containing organic pollutants after long‐term operation (90 days). Illumina MiSeq sequencing of 16S rRNA gene amplicons revealed that alpha diversity decreased after receiving nitrogen‐containing pollutants. Glucose exerted protective effects on sludge microbes, but phenol‐stimulated microbial consortia showed greater stability when without supplementation of stimulated substrates. Canonical correspondence analysis and Mantel tests indicated that the microbial community structure was significantly associated with indole and quinoline (P < 0.05). Among the dominant genera, Comamonas was the core genus in both groups for pollutants removal, while Thauera and Sphingomonas should be key functional taxa in glucose‐stimulated sludges, with Pseudoxanthomonas, Brevundimonas and Shinella in phenol‐stimulated sludges. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) analysis suggested that the important functional metabolic capabilities should be those related to Cytochrome P450 enzymes and naphthalene degradation, which were significantly correlated with the nitrogen‐containing organic pollutants (P < 0.05). CONCLUSION The results indicated that phenol‐stimulated microbial consortia could be used for efficient removal of nitrogen‐containing organic pollutants, and therefore could have potential for the bioremediation of coking wastewater. © 2018 Society of Chemical Industry

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“…GF3931, and other fungi42 as well as in bacteria Methylococcus capsulatus 29 and Diaphorobacter sp. J5-5143. Also, the purified aromatic peroxygenase from fungus Agrocybe aegerita has been found to be active towards pyridine and its derivatives30.…”

Section: Resultsmentioning

confidence: 99%

Oxyfunctionalization of pyridine derivatives using whole cells of Burkholderia sp. MAK1

Stankevičiūtė

Vaitekūnas

Petkevičius

et al. 2016

Sci Rep

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Pyridinols and pyridinamines are important intermediates with many applications in chemical industry. The pyridine derivatives are in great demand as synthons for pharmaceutical products. Moreover, pyridines are used either as biologically active substances or as building blocks for polymers with unique physical properties. Application of enzymes or whole cells is an attractive strategy for preparation of hydroxylated pyridines since the methods for chemical synthesis of pyridinols, particularly aminopyridinols, are usually limited or inefficient. Burkholderia sp. MAK1 (DSM102049), capable of using pyridin-2-ol as the sole carbon and energy source, was isolated from soil. Whole cells of Burkholderia sp. MAK1 were confirmed to possess a good ability to convert different pyridin-2-amines and pyridin-2-ones into their 5-hydroxy derivatives. Moreover, several methylpyridines as well as methylated pyrazines were converted to appropriate N-oxides. In conclusion, regioselective oxyfunctionalization of pyridine derivatives using whole cells of Burkholderia sp. MAK1 is a promising method for the preparation of various pyridin-5-ols and pyridin-N-oxides.

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Bacterial pyridine hydroxylation is ubiquitous in environment

Cited by 20 publications

References 28 publications

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

Bioremediation of nitrogen‐containing organic pollutants using phenol‐stimulated activated sludge: performance and microbial community analysis

Oxyfunctionalization of pyridine derivatives using whole cells of Burkholderia sp. MAK1

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