Bacterial Protease Treatment of Natural Rubber Latex Alters Its Primary Immunogenicity in a Mouse Model of Sensitization

Gaspari, Anthony A.; Thatcher, Thomas H.; Burns, Robert P.; Ferbel, Barbara; Tanck, Carol; Bedi, Gurrinder S.; Perrella, Frank W.

doi:10.1006/clim.2002.5262

Cited by 8 publications

(10 citation statements)

References 24 publications

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“…32 ELISA plates (Corning Glass Works) were coated with 10 ug trinitrophenyl-ovalbumin or dinitrophenyl-ovalbumin (Sigma Chemical, St Louis, Mo), and the plates were blocked with 0.25% BSA in Tween/PBS. Sera were then plated in triplicate to allow for antibody binding.…”

Section: Hapten-specific Elisamentioning

confidence: 99%

Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens

Burns

Luzina

Nasir

et al. 2005

Journal of Allergy and Clinical Immunology

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Section: Hapten-specific Elisamentioning

confidence: 99%

Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens

Burns

Luzina

Nasir

et al. 2005

Journal of Allergy and Clinical Immunology

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“…Although the ultrasound is a high‐energy consumption technique, evident advantages of using ultrasound energy can be summarized as follows: the reaction time can be reduced four times; the obtained total nitrogen content is lower; more low‐ and high‐molecular weight proteins (i.e., the disappearance of the peak in the range of 3290–3320 cm −1 in Figure 2) can be removed, which results in less protein amount in the competition for free radicals. In addition, it should be noted that proteins with MW ∼ 5–110 kDa are potent allergens, causing IgE‐mediated allergic reactions to human on one's exposure to NR products and possibly leading to severe anaphylactic reactions 43, 44. In consequence, the technique, which has the potential to remove potent allergens, may be applied to applications where the allergen elimination is of concern.…”

Section: Resultsmentioning

confidence: 99%

Prediction of styrene conversion of polystyrene/natural rubber graft copolymerization using reaction conditions: Central composite design versus artificial neural networks

Aroonsingkarat

Hansupalak

2012

J of Applied Polymer Sci

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Gross copolymer or the total product of graft copolymerization of polystyrene (PS) and rubber, prepared via emulsion polymerization using a redox initiator, is used to investigate the utilization of central composite design and artificial neural network (ANN) approaches in correlating the graft copolymerization conditions to the monomer conversion. The conditions were manipulated by changing four factors: reaction temperature and time, percentage of deproteinized natural rubber (DPNR) in the rubber mixture also containing NR, and amount of chain transfer agent. For DPNR preparation, the incorporation of ultrasound energy into a deproteinizing method (i.e., urea treatment) was preexamined. A shorter reaction time, a lower total nitrogen content, and no agglomeration of rubber particles suggest the success of the incorporation. Results exhibit that the relationship between those factors and the response can be better described by the ANN model, which is further proved to be an excellent tool for the prediction of the conversion at other reaction conditions. In addition, the thermal behavior of gross copolymer is similar to its parents, the rubber and neat PS, but more to the former owing to the larger amount of rubber component. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

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“…In addition to ammonia, the preserved latex contains small rubber particles dispersed in water and the nonrubber components such as proteins, carbohydrates and lipids . The proteins occur in the aqueous phase as well as bound to the surface of the rubber particles . These proteins are potent allergens and, therefore, the products made from NR can be allergenic .…”

Section: Introductionmentioning

confidence: 99%

“…The proteins occur in the aqueous phase as well as bound to the surface of the rubber particles . These proteins are potent allergens and, therefore, the products made from NR can be allergenic . Deproteinization of NR reduces allergenic reactions .…”

Section: Introductionmentioning

confidence: 99%

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Optimal conditions for deproteinizing natural rubber using immobilized alkaline protease

Junoi

Chisti

Hansupalak

2014

J. Chem. Technol. Biotechnol.

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BACKGROUND: Natural rubber latex contains allergenic proteins. Therefore, processes for deproteinizing the latex are needed. An immobilized enzyme process for deproteinization is reported. An optimal protocol was first developed for immobilizing a protease on cellulose−chitosan composite beads. The beads were then used in developing an optimal deproteinization treatment. The main effects and the interactions of the factors for the two processes were identified using a two-level full factorial experimental design.RESULTS: Under optimal conditions (15% cellulose in beads, immobilizing reaction pH of 9, immobilization period of 24 h), the beads attained the highest specific activity of 1685.3 U g −1 beads. Using these beads, the optimal conditions for deproteinization of the latex were: an enzyme loading of 0.1 parts per hundred of rubber (phr), a sodium dodecyl sulfate concentration of 20 phr, 30 • C, and a treatment period of 12 h. The nitrogen content of the rubber was reduced to 0.012% from an initial value of 0.3%. The enzyme beads were operationally stable and could be reused for at least five cycles. CONCLUSIONS:The optimized treatment effectively deproteinized the natural rubber. The final product was free of peptides. This deproteinization treatment was more effective than the conventional urea-based treatment performed for comparison.

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Bacterial Protease Treatment of Natural Rubber Latex Alters Its Primary Immunogenicity in a Mouse Model of Sensitization

Cited by 8 publications

References 24 publications

Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens

Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens

Prediction of styrene conversion of polystyrene/natural rubber graft copolymerization using reaction conditions: Central composite design versus artificial neural networks

Optimal conditions for deproteinizing natural rubber using immobilized alkaline protease

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