Bacterial profile of the supragingival dental biofilm in children with deciduous and early mixed dentition using next generation sequencing (HOMINGS) technique

Harris-Ricardo, Jonathan; Mercado, Luis Fang; Herrera-Herrera, Alejandra; Mesa, Natalia Fortich; Olier-Castillo, Doris; Cavanzo-Rojas, Diana; González-Quintero, Roberto

doi:10.1016/j.eimce.2019.04.005

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…Streptococcus sanguinis was found to be the bacterium with the highest relative abundance in the dentition connective tissue dysplasia (CTD) oral biofilm microbiome using next-generation sequencing (HOMINGS, Human Oral Microbe Identification using Next Generation Sequencing) technology ( Harris-Ricardo et al, 2019 ). As symbiotic members of the early colonizers in dental biofilms, the effect of the relative abundance of S.sanguinis in oral biofilms is reflected in the overall functional expression profile of dental plaque microorganisms ( Kreth et al, 2017 ), and a decrease in the relative abundance of blood streptococci is one of the hallmarks of caries development.…”

Section: Main Cariogenic Factors: Microorganismsmentioning

confidence: 99%

Association of polymicrobial interactions with dental caries development and prevention

et al. 2023

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Dental caries is a common oral disease. In many cases, disruption of the ecological balance of the oral cavity can result in the occurrence of dental caries. There are many cariogenic microbiota and factors, and their identification allows us to take corresponding prevention and control measures. With the development of microbiology, the caries-causing bacteria have evolved from the traditional single Streptococcus mutans to the discovery of oral symbiotic bacteria. Thus it is necessary to systematically organized the association of polymicrobial interactions with dental caries development. In terms of ecology, caries occurs due to an ecological imbalance of the microbiota, caused by the growth and reproduction of cariogenic microbiota due to external factors or the disruption of homeostasis by one’s own factors. To reduce the occurrence of dental caries effectively, and considering the latest scientific viewpoints, caries may be viewed from the perspective of ecology, and preventive measures can be taken; hence, this article systematically summarizes the prevention and treatment of dental caries from the aspects of ecological perspectives, in particular the ecological biofilm formation, bacterial quorum sensing, the main cariogenic microbiota, and preventive measures.

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Section: Main Cariogenic Factors: Microorganismsmentioning

confidence: 99%

Association of polymicrobial interactions with dental caries development and prevention

et al. 2023

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“…The bacterium was first isolated and identified in the Russian space station in 2004 [8] and in China in 2014. In recent years, the relationship between oral microbiota and smoking in the Chinese population, sex-specific differences in the salivary microbiome of children with active dental caries, and the analysis of bacterial characteristics of supragingival dental biofilms in deciduous and early mixed dentition children using HOMINGS sequencing technology have been mentioned [9][10][11]. Both N. sicca and N. subflava were isolated only in dental plaque of normal children.…”

Section: Discussionmentioning

confidence: 99%

Characterizing Severe Early Childhood Caries Microbiota through Culturomics

Zhao,

2023

IJCEMR

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Severe early childhood caries (SECC) is a common widespread caries affecting multiple deciduous teeth in children, which seriously affects children's physical and mental health and growth. Investigation of their pathogenic flora is essential. Two children with SECC and two caries-free children of the same age were included in this study. Using cultureomics techniques, that is, microorganisms in oral dental plaque were isolated and cultured by setting different conditions (temperature, oxygen conditions, culture medium). Species were identified by 16S rRNA sequencing, and strain function was analyzed in depth in combination with clinical information. A total of 37 species were identified in this study. 4 species had never been associated with the oral cavity before, and 3 of them had never been isolated from clinical specimens. This pilot study was able to provide some new information on the complexity of the microbiota in SECC patients versus healthy children of the same age.

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“… Dashper S.G. et al, 2019 [ 9 ] There is an ordered temporal development of the oral microbiome and a limited core oral microbiome. Harris-Ricardo J. et al, 2019 [ 28 ] Plaque in children with DMFT showed low microbiological diversity both in presence and in relative abundance in terms of genus as well as bacterial species. Dzidic M. et al, 2018 [ 29 ] Infants born by C-section had initially skewed bacterial content compared with vaginally delivered infants, but this was recovered with age.…”

Section: Table A1mentioning

confidence: 99%

Exploring Oral Microbiome in Healthy Infants and Children: A Systematic Review

D’Agostino

Ferrara

Valentini

et al. 2022

IJERPH

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Recent advances in the development of next-generation sequencing (NGS) technologies, such as the 16S rRNA gene sequencing, have enabled significant progress in characterizing the architecture of the oral microbiome. Understanding the taxonomic and functional components of the oral microbiome, especially during early childhood development, is becoming critical for identifying the interactions and adaptations of bacterial communities to dynamic conditions that may lead to the dysfunction of the host environment, thereby contributing to the onset and/or progression of a wide range of pathological conditions. We aimed to provide a comprehensive overview of the most recent evidence from studies of the oral microbiome of infants and young children, focusing on the development of oral microbiome in the window of birth to 18 years, focusing on infants. A systematic literature search was conducted in PubMed, Scopus, WOS, and the WHO clinical trial website for relevant articles published between 2006 to 2022 to identify studies that examined genome-wide transcriptome of the oral microbiome in birth, early childhood, and adolescence performed via 16s rRNA sequence analysis. In addition, the references of selected articles were screened for other relevant studies. This systematic review was performed in accordance PRISMA guidelines. Data extraction and quality assessment were independently conducted by two authors, and a third author resolved discrepancies. Overall, 34 studies were included in this systematic review. Due to a considerable heterogeneity in study population, design, and outcome measures, a formal meta-analysis was not carried out. The current evidence indicates that a core microbiome is present in newborns, and it is stable in species number. Disparity about delivery mode influence are found. Further investigations are needed.

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Bacterial profile of the supragingival dental biofilm in children with deciduous and early mixed dentition using next generation sequencing (HOMINGS) technique

Cited by 7 publications

References 16 publications

Association of polymicrobial interactions with dental caries development and prevention

Association of polymicrobial interactions with dental caries development and prevention

Characterizing Severe Early Childhood Caries Microbiota through Culturomics

Exploring Oral Microbiome in Healthy Infants and Children: A Systematic Review

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