Bacterial profile from caecal contents and soft faeces in growing rabbits given diets differing in soluble and insoluble fibre levels

Rodríguez-Romero, N.; Abecia, Leticia; Fondevila, M.

doi:10.1016/j.anaerobe.2012.10.006

Cited by 11 publications

(10 citation statements)

References 30 publications

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“…Therefore, the present study explored in vivo the probiotic properties of Lactobacillus casei in suckling rabbit. The development of intestinal microflora begins at the day of birth and is affected by many factors [23], including the bacterial structures in the mother's birth canal or milk, so we designed a paired experiment and artificially fed suckling rabbits from the fifth day after their birth to minimize the influence from their mother. Kits were not separated from their mother before 5 days old because we found that kits could not survive if they did not suck their mother's colostrum.…”

Section: Discussionmentioning

confidence: 99%

Orally administered Lactobacillus casei exhibited several probiotic properties in artificially suckling rabbits

Shen

Cui

2020

Asian-Australas J Anim Sci

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Objective: Lactobacilli in rabbit intestine is rare and its function in rabbit gut health is not fully understood. The present study aimed to evaluate in vivo the probiotic potential of Lactobacillus casei for suckling rabbits.Methods: Two healthy 5-day-old suckling rabbits with similar weights from each of 12 New Zealand White litters were selected and disturbed to control group and treatment group. All rabbits were artificially fed. The treatment group had been supplemented with live Lactobacillus casei in the milk from the beginning of the trial to 13 days of age. At 15 days of age, healthy paired rabbits were slaughtered to collect intestinal samples.Results: i) Oral administration of Lactobacillus casei significantly increased the proportion of Lactobacilli in the total intestinal bacteria (p<0.01) and obviously reduced that of Escherichia-Shigella (p<0.01); ii) treatment increased the length of vermiform appendix (p<0.05); iii) a higher percentage of degranulated paneth cells was observed in the duodenum and jejunum when rabbits administered with Lactobacillus casei (p<0.01); and iv) the expression of tolllike receptor 9, lysozyme (LYZ), and defensin-7-like (DEFEN) in the duodenum and jejunum was stimulated by supplemented Lactobacillus casei (p<0.05).Conclusion: Orally administered Lactobacillus casei could increase the abundance of intestinal Lactobacilli, decrease the relative abundance of intestinal Escherichia-Shigella, promote the growth of appendix vermiform, stimulate the degranulation of paneth cells and induce the expression of DEFEN and LYS. The results of the present study implied that Lactobacillus casei exhibited probiotic potential for suckling rabbits.

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Section: Discussionmentioning

confidence: 99%

Orally administered Lactobacillus casei exhibited several probiotic properties in artificially suckling rabbits

Shen

Cui

2020

Asian-Australas J Anim Sci

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“…Capillary electrophoresis single-stranded conformation polymorphism (CE-SSCP) and denaturing gradient gel electrophoresis (DGGE) were useful to determine the similarity between bacterial populations of the cecal content and soft faeces [20] on diets with different fibre content [21] and to draw a general quantitative map of the evolution of the microbiota along the rabbit's life [4]. Cloning of 16S RNA genes in Escherichia coli offered a more precise identification of the bacterial inhabitants of rabbit's gut, however, the procedure introduced qualitative and quantitative biases.…”

Section: Discussionmentioning

confidence: 99%

“…Cloning of 16S RNA genes in Escherichia coli offered a more precise identification of the bacterial inhabitants of rabbit's gut, however, the procedure introduced qualitative and quantitative biases. In a first inventory, Abecia et al (2005) [21] showed the relevance in rabbit's cecal content of Clostridia (Lachnospiraceae, Ruminococcaceae) and Verrucomicrobia, and reported a number of new unidentified sequences. Authors described the absence of sequences of the Prevotella – Bacteroides group, however, nowadays identification databases have been enriched and we could classify all those sequences (Group 5, in Abecia et al, 2005), in the Bacteroidetes phylum (Table S13).…”

Section: Discussionmentioning

confidence: 99%

Changes in Cecal Microbiota and Mucosal Gene Expression Revealed New Aspects of Epizootic Rabbit Enteropathy

et al. 2014

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Epizootic Rabbit Enteropathy (ERE) is a severe disease of unknown aetiology that mainly affects post-weaning animals. Its incidence can be prevented by antibiotic treatment suggesting that bacterial elements are crucial for the development of the disease. Microbial dynamics and host responses during the disease were studied. Cecal microbiota was characterized in three rabbit groups (ERE-affected, healthy and healthy pretreated with antibiotics), followed by transcriptional analysis of cytokines and mucins in the cecal mucosa and vermix by q-rtPCR. In healthy animals, cecal microbiota with or without antibiotic pretreatment was very similar and dominated by Alistipes and Ruminococcus. Proportions of both genera decreased in ERE rabbits whereas Bacteroides, Akkermansia and Rikenella increased, as well as Clostridium, γ-Proteobacteria and other opportunistic and pathogenic species. The ERE group displayed remarkable dysbiosis and reduced taxonomic diversity. Transcription rate of mucins and inflammatory cytokines was very high in ERE rabbits, except IL-2, and its analysis revealed the existence of two clearly different gene expression patterns corresponding to Inflammatory and (mucin) Secretory Profiles. Furthermore, these profiles were associated to different bacterial species, suggesting that they may correspond to different stages of the disease. Other data obtained in this work reinforced the notion that ERE morbidity and mortality is possibly caused by an overgrowth of different pathogens in the gut of animals whose immune defence mechanisms seem not to be adequately responding.

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“…However, it is difficult to study the influence of any dietary component on the microbiota, as traditional cultivation techniques only recover around one-quarter of the intestinal microbiota. Now, molecular microbiology provides evidence of the age-related changes for the intestinal microbiota, while the diet seems to have a minor impact (Rodríguez- Romero et al, 2012;Combes et al, 2013Combes et al, , 2017, although some relevant effects related to DF were also reported (Gómez-Conde et al, 2007. Other indirect techniques are used, such as the VFA concentration, microbial nitrogen synthesized or fibrolytic activity.…”

Section: Microbial Activitymentioning

confidence: 99%

Fibre digestion.

Carabaño¹,

Abad-Guamán²,

Garcia³

et al. 2020

Nutrition of the Rabbit

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Bacterial profile from caecal contents and soft faeces in growing rabbits given diets differing in soluble and insoluble fibre levels

Cited by 11 publications

References 30 publications

Orally administered Lactobacillus casei exhibited several probiotic properties in artificially suckling rabbits

Orally administered Lactobacillus casei exhibited several probiotic properties in artificially suckling rabbits

Changes in Cecal Microbiota and Mucosal Gene Expression Revealed New Aspects of Epizootic Rabbit Enteropathy

Fibre digestion.

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