Bacterial Production of Metal(loid) Nanostructures

Pradhan, Nikhil; Turner, Raymond J.

doi:10.1007/978-3-030-97185-4_7

Cited by 4 publications

(3 citation statements)

References 152 publications

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“…The standard redox potential of Ag is well within the physiological range ( Table 6 ), and Ag nanoparticles are formed by bacteria, fungi, and plant extracts ( 54 ). The Ag(I) cation is very sensitive against light and forms complexes with very low solubility with chloride and sulfide, which also affects the standard potential ( Table 6 ).…”

Section: Discussionmentioning

confidence: 99%

A gold speciation that adds a second layer to synergistic gold-copper toxicity in Cupriavidus metallidurans

Hirth,

Wiesemann,

Krüger

et al. 2024

Appl Environ Microbiol

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The metal-resistant bacterium Cupriavidus metallidurans occurs in metal-rich environments. In auriferous soils, the bacterium is challenged by a mixture of copper ions and gold complexes, which exert synergistic toxicity. The previously used, self-made Au(III) solution caused a synergistic toxicity of copper and gold that was based on the inhibition of the CupA-mediated efflux of cytoplasmic Cu(I) by Au(I) in this cellular compartment. In this publication, the response of the bacterium to gold and copper was investigated by using a commercially available Au(III) solution instead of the self-made solution. The new solution was five times more toxic than the previously used one. Increased toxicity was accompanied by greater accumulation of gold atoms by the cells. The contribution of copper resistance determinants to the commercially available Au(III) solution and synergistic gold-copper toxicity was studied using single- and multiple-deletion mutants. The commercially available Au(III) solution inhibited periplasmic Cu(I) homeostasis, which is required for the allocation of copper ions to copper-dependent proteins in this compartment. The presence of the gene for the periplasmic Cu(I) and Au(I) oxidase, CopA, decreased the cellular copper and gold content. Transcriptional reporter gene fusions showed that up-regulation of gig , encoding a minor contributor to copper resistance, was strictly glutathione dependent. Glutathione was also required to resist synergistic gold-copper toxicity. The new data indicated a second layer of synergistic copper-gold toxicity caused by the commercial Au(III) solution, inhibition of the periplasmic copper homeostasis in addition to the cytoplasmic one. IMPORTANCE When living in auriferous soils, Cupriavidus metallidurans is not only confronted with synergistic toxicity of copper ions and gold complexes but also by different gold species. A previously used gold solution made by using aqua regia resulted in the formation of periplasmic gold nanoparticles, and the cells were protected against gold toxicity by the periplasmic Cu(I) and Au(I) oxidase CopA. To understand the role of different gold species in the environment, another Au(III) solution was commercially acquired. This compound was more toxic due to a higher accumulation of gold atoms by the cells and inhibition of periplasmic Cu(I) homeostasis. Thus, the geo-biochemical conditions might influence Au(III) speciation. The resulting Au(III) species may subsequently interact in different ways with C. metallidurans and its copper homeostasis system in the cytoplasm and periplasm. This study reveals that the geochemical conditions may decide whether bacteria are able to form gold nanoparticles or not.

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Section: Discussionmentioning

confidence: 99%

A gold speciation that adds a second layer to synergistic gold-copper toxicity in Cupriavidus metallidurans

Hirth,

Wiesemann,

Krüger

et al. 2024

Appl Environ Microbiol

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“…In addition, bacteria‐driven synthesis allows MeNPs capping with natural and biocompatible molecules that prevent aggregation and help in biomedical applications of the nanoparticles. Many bacterial species and strains have been described to date with good abilities to produce MeNPs (Gallo & Schillaci, 2021 ; Ghosh et al, 2021 ; Pradhan & Turner, 2022 ). Nevertheless, exploring new biofactories for MeNPs production is crucial to bypass some of the existing limitations (Figure 1 ).…”

Section: Searching For the Bacterial Chassis For Menps ...mentioning

confidence: 99%

Opportunities and obstacles in microbial synthesis of metal nanoparticles

Carmona

Poblete‐Castro

Rai

et al. 2023

Microbial Biotechnology

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Metallic nanoparticles (MeNPs) are widely used in many areas such as biomedicine, packaging, cosmetics, colourants, agriculture, antimicrobial agents, cleaning products, as components of electronic devices and nutritional supplements. In addition, some MeNPs exhibit quantum properties, making them suitable materials in the photonics, electronic and energy industries. Through the lens of technology, microbes can be considered nanofactories capable of producing enzymes, metabolites and capping materials involved in the synthesis, assembly and stabilization of MeNPs. This bioprocess is considered more ecofriendly and less energy intensive than the current chemical synthesis routes. However, microbial synthesis of MeNPs as an alternative method to the chemical synthesis of nanomaterials still faces some challenges that need to be solved. Some of these challenges are described in this Editorial.

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“…The selection of vanadium and iron in the given VNFe@C nanozyme was inspired by natural peroxidases (Scheme ). These natural peroxidases have been classified in two groups depending on the type of their cofactor or active catalytic site, i.e., heme (iron-containing porphyrin ring) and vanadium. , Our proposition was that when these two specific metal atoms are incorporated in the nitrogen-doped carbon structure, it will have better activity than natural peroxidases such as horseradish peroxidase due to the synergistic effects of dual active catalytic sites that mimic natural peroxidases. Vanadium is also an interesting element with the highest valence states that can be tuned to attain a wide range of redox properties for various applications.…”

Section: Introductionmentioning

confidence: 99%

PBA-Derived Heteroatom-Doped Mesoporous Graphitic Spheroids as Peroxidase Nanozyme for In Vitro Tumor Cells Detection

Sahar,

Zeb,

Mao

et al. 2024

ACS Appl. Bio Mater.

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Inspired by the two kinds of naturally occurring peroxidases (POD) with vanadium or heme (iron)-based active catalytic centers, we have developed a dual metal-based nanozyme with dual V and Fe-based active catalytic centers. Co-doping of graphene with heteroatoms has a synergistic effect on the catalytic properties of the nanomaterial as the distances of migration of the substrates drastically reduce. However, a few studies have reported the codoping of heterometallic elements in the graphene structure due to the complexity of the synthesis procedures. Herein, we report the synthesis of in situ doped bimetallic VNFe@C mesoporous graphitic spheroids nanozyme via pyrolysis without the assistance of any template assisted method. The Prussian-blue analog-based precursor material was synthesized by a facile one-step low-temperature synthesis procedure. The bimetallic spheroids showed an excellent affinity toward H 2 O 2 , with a K m value of 0.26 mM when compared to 0.436 for the natural POD, which is much better than the natural POD, which was utilized to detect tumor cells in vitro through the intracellular H 2 O 2 produced by these cells under high oxidative stress. The VNFe@C mesoporous spheroids generate dual reactive oxygen species, including the • OH and • O 2 H − radicals, in the presence of H 2 O 2 , which are responsible for the POD-like activity of these nanozymes, while the bimetallic V/Fe doping plays a synergistic role in the enhancement of the activity of codoped graphitic spheroids.

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Bacterial Production of Metal(loid) Nanostructures

Cited by 4 publications

References 152 publications

A gold speciation that adds a second layer to synergistic gold-copper toxicity in Cupriavidus metallidurans

A gold speciation that adds a second layer to synergistic gold-copper toxicity in Cupriavidus metallidurans

Opportunities and obstacles in microbial synthesis of metal nanoparticles

PBA-Derived Heteroatom-Doped Mesoporous Graphitic Spheroids as Peroxidase Nanozyme for In Vitro Tumor Cells Detection

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