Bacterial population and innate immunity-related genes in rat gastrointestinal tract are altered by vitamin A-deficient diet

Amit-Romach, Einat; Uni, Zehava; Cheled, Shira; Berkovich, Zipi; Reifen, Ram

doi:10.1016/j.jnutbio.2008.01.002

Cited by 66 publications

(54 citation statements)

References 52 publications

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“…It has been reported that VA deficiency also decreases the levels of intestinal IgA antibodies, defensins, and mucus, allowing easier translocation of bacteria or bacterial factors (48)(49)(50). Thus, it is reasonable to speculate that larger quantities of pathogenic bacterial factors, including Stx2 and lipopolysaccharide (LPS), would gain access to the bloodstream in VA-D mice during EHEC infection, suggesting that gut alterations in VA-D mice tend to decrease the huge difference in survival observed after the i.v.…”

Section: Discussionmentioning

confidence: 99%

Retinoid Levels Influence Enterohemorrhagic Escherichia coli Infection and Shiga Toxin 2 Susceptibility in Mice

et al. 2014

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e Enterohemorrhagic Escherichia coli (EHEC) is a food-borne pathogen that produces Shiga toxin (Stx) and causes hemorrhagic colitis. Under some circumstances, Stx produced within the intestinal tract enters the bloodstream, leading to systemic complications that may cause the potentially fatal hemolytic-uremic syndrome. Although retinoids like vitamin A (VA) and retinoic acid (RA) are beneficial to gut integrity and the immune system, the effect of VA supplementation on gastrointestinal infections of different etiologies has been controversial. Thus, the aim of this work was to study the influence of different VA status on the outcome of an EHEC intestinal infection in mice. We report that VA deficiency worsened the intestinal damage during EHEC infection but simultaneously improved survival. Since death is associated mainly with Stx toxicity, Stx was intravenously inoculated to analyze whether retinoid levels affect Stx susceptibility. Interestingly, while VA-deficient (VA-D) mice were resistant to a lethal dose of Stx2, RA-supplemented mice were more susceptible to it. Given that peripheral blood polymorphonuclear cells (PMNs) are known to potentiate Stx2 toxicity, we studied the influence of retinoid levels on the absolute number and function of PMNs. We found that VA-D mice had decreased PMN numbers and a diminished capacity to produce reactive oxygen species, while RA supplementation had the opposite effect. These results are in line with the well-known function of retinoids in maintaining the homeostasis of the gut but support the idea that they have a proinflammatory effect by acting, in part, on the PMN population.

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Section: Discussionmentioning

confidence: 99%

Retinoid Levels Influence Enterohemorrhagic Escherichia coli Infection and Shiga Toxin 2 Susceptibility in Mice

et al. 2014

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“…Statistical analyses between DF, NEC, and B. bifidum groups were performed by ANOVA followed by Fisher paired least significant difference test. The 2 test was utilized to analyze difference in incidence of disease. All statistical analyses were conducted by use of the statistical program StatView for Macintosh computers (Abacus Concepts, Berkeley, CA).…”

Section: Methodsmentioning

confidence: 99%

“…There are two main classes of mucins: 1) secreted gel-forming mucins and 2) membrane-bound mucins (54). In the rat small intestine, Muc2 is the predominant secretory mucin produced by goblet cells, whereas Muc3 is the major membrane-bound mucin detected in goblet cells and enterocytes (1,2). Mucins are cosecreted with TFFs, small peptides exerting multiple biological effects on epithelium.…”

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confidence: 99%

Bifidobacterium bifidum improves intestinal integrity in a rat model of necrotizing enterocolitis

Khailová¹,

Dvorak

Arganbright³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

164

159

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“…Essa maior população no número de cé-lulas caliciformes no grupo tratado com composto vegetal provavelmente induz em maior quantidade de muco e isso pode ser importante para controlar bactérias patogênicas. De acordo com Amit-Romach et al (2009), a redução na produção de muco possibilita melhores condições de adesão e proliferação de bactérias patogênicas em contrapartida da redução de bactérias benéϐicas ao organismo.…”

Section: Discussionunclassified

Óleo essencial de orégano, alecrim, canela e extrato de pimenta no controle de Salmonella, Eimeria e Clostridium em frangos de corte

et al. 2012

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Este trabalho foi conduzido com o objetivo de avaliar a eficiência de um composto vegetal contendo óleo essencial de orégano, alecrim, canela e extrato de pimenta vermelha no controle de Salmonella, Eimeria e Clostridium em frangos de corte. Para tal, foram realizados dois experimentos. No primeiro avaliou-se a eficiência deste produto no controle de Clostridium perfringens após desafio com Eimeria acervulina, E. maxima e E. tenella. Aves de um dia de idade foram divididas em três grupos: T1 - dieta controle sem aditivo promotor de crescimento; T2 - dieta com adição de avilamicina (10ppm); e T3 - dieta com adição do composto vegetal (100ppm). O uso do composto vegetal na alimentação de frangos reduziu lesões específicas de E. maxima e E. tenella aos 14 dias pós-inoculação (PI) como também reduziram a contagem de unidades formadoras de colônias (UFC) de Clostridium perfringens no conteúdo do ceco das aves em relação ao grupo controle. No segundo experimento avaliou-se a eficiência deste mesmo produto em aves desafiadas com Salmonella Enteritidis. Aves de um dia de idade foram distribuídas em três tratamentos, sendo T1 - dieta controle sem adição de antibiótico promotor de crescimento, T2 - dieta com 10ppm de Avilamicina, T3 - dieta com 100ppm de um produto a base do composto vegetal acima citado. Aos 21 dias de idade todas as aves foram inoculadas com 10(5) UFC de Salmonella Enteritidis. A utilização do composto vegetal e avilamicina diminuiu a excreção de Salmonella nas aves 72 horas PI de Salmonella. A utilização do composto vegetal aumentou a relação vilo/células CD3+ no duodeno, em relação ao grupo avilamicina e controle, porém não teve efeito sobre a expressão destas células no ceco.

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Bacterial population and innate immunity-related genes in rat gastrointestinal tract are altered by vitamin A-deficient diet

Cited by 66 publications

References 52 publications

Retinoid Levels Influence Enterohemorrhagic Escherichia coli Infection and Shiga Toxin 2 Susceptibility in Mice

Retinoid Levels Influence Enterohemorrhagic Escherichia coli Infection and Shiga Toxin 2 Susceptibility in Mice

Bifidobacterium bifidum improves intestinal integrity in a rat model of necrotizing enterocolitis

Óleo essencial de orégano, alecrim, canela e extrato de pimenta no controle de Salmonella, Eimeria e Clostridium em frangos de corte

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