Bacterial Polysaccharides in the Diagnosis of Infections the Polysaccharide Lysis Test

Some Data Regarding the Mechanism of Indirect Bacterial Haemagglutination and Haemagglutination Inhibition

“…We are in a position to confirm the correctness of certain lit erary references (11,14) according to which sometimes even untreated extracts may be found to have the desired sensitizing effect, although-as a general rule-heating to 100°C or treat ment with alkali enhances their sensitizing power very consider ably. While heat treatment was observed to step up this power in every case, the effect of a treatment with N/4 NaOH not only lagged somewhat behind that of a treatment with heat but in some cases actually diminished both sensitizing capacity and in hibitory activity.…”

“…Results are listed in Table I. According to a number of authors (13)(14)(15)(16)(17), the sensitizing capacity of antigens can be enhanced also by a treatment with alkali. To examine the matter we studied the sensitizing power of dialysed Boivin extracts made from Sh.…”

“…It is known lhal both heating (5, (5, 10, 11, 12, 13) and alkali-treatment (13,14,15,16,17) are suited to enhance the sensitizing activity of bacterial ex tracts. Therefore, we had also to decide as to which of these treatments would confer the higher sensitizing power on the ex tracts.…”