Bacterial plasmids: replication of extrachromosomal genetic elements encoding resistance to antimicrobial compounds

Actis, Luis A.; Tolmasky, Marcelo E.; Crosa, Jorge H.

doi:10.2741/actis

Cited by 51 publications

(13 citation statements)

References 205 publications

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“…We do not know the potential functions of the accessory genes on ColE1-4kb, but their compatibility with ColE1-6kb may stem from differences in their RNAI sequences [77] and their mob region. The ColE1 plasmid family represents plasmids that are only transmissible in the presence of an additional plasmid with conjugative functions and usually carry a mob region encoding specific relaxase and accessory mob genes ( mbeB , mbeD mbeC ).…”

Section: Discussionmentioning

confidence: 99%

Hotspot mutations and ColE1 plasmids contribute to the fitness of Salmonella Heidelberg in poultry litter

et al. 2018

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Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg) is a clinically-important serovar linked to food-borne illness, and commonly isolated from poultry. Investigations of a large, multistate outbreak in the USA in 2013 identified poultry litter (PL) as an important extra-intestinal environment that may have selected for specific S. Heidelberg strains. Poultry litter is a mixture of bedding materials and chicken excreta that contains chicken gastrointestinal (GI) bacteria, undigested feed, feathers, and other materials of chicken origin. In this study, we performed a series of controlled laboratory experiments which assessed the microevolution of two S. Heidelberg strains (SH-2813 and SH-116) in PL previously used to raise 3 flocks of broiler chickens. The strains are closely related at the chromosome level, differing from the reference genome by 109 and 89 single nucleotide polymorphisms/InDels, respectively. Whole genome sequencing was performed on 86 isolates recovered after 0, 1, 7 and 14 days of microevolution in PL. Only strains carrying an IncX1 (37kb), 2 ColE1 (4 and 6kb) and 1 ColpVC (2kb) plasmids survived more than 7 days in PL. Competition experiments showed that carriage of these plasmids was associated with increased fitness. This increased fitness was associated with an increased copy number of IncX1 and ColE1 plasmids. Further, all Col plasmid-bearing strains had hotspot mutations in 37 loci on the chromosome and in 3 loci on the IncX1 plasmid. Additionally, we observed a decrease in susceptibility to tobramycin, kanamycin, gentamicin, neomycin and fosfomycin for Col plasmid-bearing strains. Our study demonstrates how positive selection from poultry litter can change the evolutionary path of S. Heidelberg.

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Section: Discussionmentioning

confidence: 99%

Hotspot mutations and ColE1 plasmids contribute to the fitness of Salmonella Heidelberg in poultry litter

et al. 2018

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“…Bacterial plasmids play a key role in a variety of traits like drug resistance, virulence, and the metabolism of rare substrates under specific conditions (Actis et al . 1999). Plasmids are able to mobilize these traits between different strains and thus play an important role in horizontal gene transfer.…”

Section: Discussionmentioning

confidence: 99%

Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC)

et al. 2011

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The genome sequences of two Escherichia coli O104:H4 strains derived from two different patients of the 2011 German E. coli outbreak were determined. The two analyzed strains were designated E. coli GOS1 and GOS2 (German outbreak strain). Both isolates comprise one chromosome of approximately 5.31 Mbp and two putative plasmids. Comparisons of the 5,217 (GOS1) and 5,224 (GOS2) predicted protein-encoding genes with various E. coli strains, and a multilocus sequence typing analysis revealed that the isolates were most similar to the entero-aggregative E. coli (EAEC) strain 55989. In addition, one of the putative plasmids of the outbreak strain is similar to pAA-type plasmids of EAEC strains, which contain aggregative adhesion fimbrial operons. The second putative plasmid harbors genes for extended-spectrum β-lactamases. This type of plasmid is widely distributed in pathogenic E. coli strains. A significant difference of the E. coli GOS1 and GOS2 genomes to those of EAEC strains is the presence of a prophage encoding the Shiga toxin, which is characteristic for enterohemorrhagic E. coli (EHEC) strains. The unique combination of genomic features of the German outbreak strain, containing characteristics from pathotypes EAEC and EHEC, suggested that it represents a new pathotype Entero-Aggregative-Haemorrhagic Escherichiacoli (EAHEC).Electronic supplementary materialThe online version of this article (doi:10.1007/s00203-011-0725-6) contains supplementary material, which is available to authorized users.

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“…They can be either conjugative, encoding mechanisms for selftransfer by conjugation, or non-conjugative and hence incapable of initiating conjugation for self-transmission. In addition, plasmids are assigned to incompatibility (Inc) groups, depending on their ability to co-exist in the same cell line (Novick, 1987;Actis et al, 1998). Incompatible plasmids belong to the same incompatibility group.…”

Section: Introductionmentioning

confidence: 99%

Rule-based modelling of conjugative plasmid transfer and incompatibility

Gregory

Saunders

2008

Biosystems

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Bacterial plasmids: replication of extrachromosomal genetic elements encoding resistance to antimicrobial compounds

Cited by 51 publications

References 205 publications

Hotspot mutations and ColE1 plasmids contribute to the fitness of Salmonella Heidelberg in poultry litter

Hotspot mutations and ColE1 plasmids contribute to the fitness of Salmonella Heidelberg in poultry litter

Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC)

Rule-based modelling of conjugative plasmid transfer and incompatibility

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