Bacterial plasmids and gene flux

Amábile-Cuevas, Carlos F.; Chicurel, Marina

doi:10.1016/0092-8674(92)90095-t

Cited by 123 publications

(82 citation statements)

References 75 publications

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“…Bacterial conjugation is an important means of genetic exchange between members of the same and sometimes taxonomically unrelated species (Guiney 1993). For clinical bacteria, conjugation is considered the primary means of dissemination of antibiotic resistance (Amabile-Cuevas and Chicurel 1992). This paper reports the conjugal transfer frequencies of streptomycin R plasmids that were detected in Ps.…”

Section: Introductionmentioning

confidence: 99%

Characterization of plasmids that encode streptomycin‐resistance in bacterial epiphytes of apple

Huang

Burr

1999

Journal of Applied Microbiology

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T .-C. H UA NG A ND T. J . B UR R . 1999. Streptomycin resistance in strains of Pseudomonas syringae pv. papulans, Pantoea agglomerans and a yellow-pigmented, nonfluorescent Pseudomonas sp. (Py), isolated from apple orchards in New York and Washington states, is predominantly associated with strA-strB genes carried on conjugal plasmids (R plasmids). None of 128 resistant Erwinia amylovora strains from the eastern and western USA hybridized with a strA-strB probe, SMP3. Resistant Py strains transfered R plasmids to Ps. syringae pv. papulans and to Py in vitro at frequencies of 10 −1 -10 −2 per recipient cell whereas Ps. syringae pv. papulans transferred its plasmids at frequencies of 10 −2 to below detectable levels. Transfer of R plasmids to P. agglomerans was not detected and resistant P. agglomerans did not transfer their R plasmids to any recipients. R plasmids were found to be highly diverse as measured by DNA fingerprint analysis. Transfer-deficient transposon mutants of R plasmid pCPP519 were generated, and 3·9 kb EcoRI and 3·0 kb SmaI fragments that hybridized with a Tn5 probe were cloned and sequenced. The deduced amino acid sequences of the 3·9 kb fragment were similar to proteins involved in replication, nicking at oriT, and piliation in other bacteria.

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Section: Introductionmentioning

confidence: 99%

Characterization of plasmids that encode streptomycin‐resistance in bacterial epiphytes of apple

Huang

Burr

1999

Journal of Applied Microbiology

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“…The exchange of plasmid and chromosomal DNA by conjugation is a significant factor in the genetic variation of bacteria (1,22,26). The evolutionary impact of conjugation has been demonstrated most dramatically by the rapid emergence and dissemination of multiple-antibiotic resistance in bacterial strains through the transfer of R plasmids (1,24).…”

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confidence: 99%

“…The evolutionary impact of conjugation has been demonstrated most dramatically by the rapid emergence and dissemination of multiple-antibiotic resistance in bacterial strains through the transfer of R plasmids (1,24). The enormous potential for genetic variation in bacteria by conjugation is perhaps best illustrated by the promiscuous plasmids of incompatibility group P (IncP) (28,43), which are capable of directing the transfer of DNA to a wide variety of gram-negative and gram-positive bacterial species and even to several yeast species (11,13).…”

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confidence: 99%

Mechanism of retrotransfer in conjugation: prior transfer of the conjugative plasmid is required

1996

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“…Plasmids are present in nearly all bacterial species (Amabile-Cuevas & Chicurel, 1992), range in size from a few to more than 1000 kbp and, as such, can represent a large proportion of the whole bacterial genome. In nature, plasmids appear to increase bacterial genetic diversity and to promote bacterial adaptation by horizontal gene spread Gogarten et al, 2002;.…”

Section: The Plasmid Genome Databasementioning

confidence: 99%