Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses

Hirota, Ryuichi; Kuroda, Akio; Kato, Junichi; Ohtake, Hisao

doi:10.1016/j.jbiosc.2009.10.018

Cited by 108 publications

(80 citation statements)

References 59 publications

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“…To construct a mutant deficient in Pi and organic Pi transport, three organic Pi transporters ( glpT, ugpB , and uhpT ) were disrupted in addition to four Pi transporters ( pitA, pitB, phnCDE , and pstSCAB )57. The gene for phoA was also disrupted because E. coli alkaline phosphatase exhibits weak Pt-oxidizing activity58 which could result in oxidization of Pt in the periplasmic space, reducing the amount of Pt taken up.…”

Section: Methodsmentioning

confidence: 99%

A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite

Hirota

Abe

Katsuura

et al. 2017

Sci Rep

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There is a growing demand to develop biocontainment strategies that prevent unintended proliferation of genetically modified organisms in the open environment. We found that the hypophosphite (H3PO2, HPt) transporter HtxBCDE from Pseudomonas stutzeri WM88 was also capable of transporting phosphite (H3PO3, Pt) but not phosphate (H3PO4, Pi), suggesting the potential for engineering a Pt/HPt-dependent bacterial strain as a biocontainment strategy. We disrupted all Pi and organic Pi transporters in an Escherichia coli strain expressing HtxABCDE and a Pt dehydrogenase, leaving Pt/HPt uptake and oxidation as the only means to obtain Pi. Challenge on non-permissive growth medium revealed that no escape mutants appeared for at least 21 days with a detection limit of 1.94 × 10−13 per colony forming unit. This represents, to the best of our knowledge, the lowest escape frequency among reported strategies. Since Pt/HPt are ecologically rare and not available in amounts sufficient for the growth of the Pt/HPt-dependent bacteria, this strategy offers a reliable and practical method for biocontainment.

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Section: Methodsmentioning

confidence: 99%

A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite

Hirota

Abe

Katsuura

et al. 2017

Sci Rep

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“…To determine the transformation of poly-P, cultures sampled under each condition were further analyzed by 31 P NMR. Transmission electron microscopy (TEM, FEI Tecnai F20, Netherlands) was also adopted to observe the changes of intracellular particulate granule (Hirota et al 2010). …”

Section: Ortho-p Starvation and Recoverymentioning

confidence: 99%

“…Besides the accumulation of phosphorus in EPS, a large amount of ortho-P was normally removed accompanying with the cell growth (Hirota et al 2010). However, the quantity and speciation of phosphorus in bacterial EPS and cells were rarely figured out.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous denitrification and phosphorus removal by Agrobacterium sp. LAD9 under varying oxygen concentration

Chen

Gui

et al. 2015

Appl Microbiol Biotechnol

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Although efficient aerobic denitrification has received increasing attention, few studies have been made on simultaneous denitrification and phosphorus removal (SDPR) under aerobic condition. In this study, SDPR by an efficient aerobic denitrifier, Agrobacterium sp. LAD9, was firstly demonstrated. High nitrate and phosphorus removal rates of 7.50 and 1.02 mg L(-1) h(-1) were achieved in wide range of O2 concentration from 5.92 to 20.02 mg L(-1). The N2O production would be inhibited as O2 concentration exceeded 11.06 mg L(-1), while the phosphorus removal efficiency would be generally improved with increasing O2 concentration. (15)N mass spectrometry revealed that nitrogen removal accorded with the typical aerobic denitrification pathway, while (31)P nuclear magnetic resonance spectroscopy ((31)P NMR) indicated the fate of phosphorus to cells, extracellular polymeric substances (EPS), and polyphosphate (poly-P) of the denitrifier. EPS acted as a reservoir of phosphorus and the transformation of poly-P was dynamic and depended on initial orthophosphate (ortho-P) content. The aerobic SDPR would greatly simplify the conventional wastewater treatment processes which required separated considerations of nitrogen and phosphorus removal.

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“…Phosphorus (P) is one of the most critical elements in biological building blocks and plays an essential role in the cellular metabolism of all living organisms (Hirota et al, 2010). Since no other element can substitute for P in biochemical processes, humans ultimately rely on P availability.…”

Section: Introductionmentioning

confidence: 99%

A mobile pilot-scale plant for in situ demonstration of phosphorus recovery from wastewater using amorphous calcium silicate hydrates

Okano

Miyamaru

Yamamoto

et al. 2016

Separation and Purification Technology

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Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses

Cited by 108 publications

References 59 publications

A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite

A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite

Simultaneous denitrification and phosphorus removal by Agrobacterium sp. LAD9 under varying oxygen concentration

A mobile pilot-scale plant for in situ demonstration of phosphorus recovery from wastewater using amorphous calcium silicate hydrates

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