Bacterial PerO Permeases Transport Sulfate and Related Oxyanions

Hoffmann, Marie-Christine; Pfänder, Yvonne; Tintel, Marc; Masepohl, Bernd

doi:10.1128/jb.00183-17

Cited by 9 publications

(8 citation statements)

References 38 publications

(42 reference statements)

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“…Three Na + ions are coupled to the transport of one sulfate molecule resulting in electrogenic transport and the net transfer of one positive charge across the membrane ( Pajor, 2006 ). A DASS family sulfate transporter (YP_003577054) was identified and characterized in the Alphaproteobacterium R. capsulatum ( Gisin et al, 2010 ; Hoffmann et al, 2017 ). Recently, more DASS family sulfate transporters were characterized in the assimilatory sulfate-reducing Alphaproteobacteria Rhodobacter sphaeroides , Sinorhizobium melioti , Agrobacterium tumefaciens , Dinoroseobacter shibae and the gammaproteobacterium Pseudomonas stutzeri ( Hoffmann et al, 2017 ).…”

Section: Sulfate Transporters In Dissimilatory Sulfate Reducing Bactementioning

confidence: 99%

“…A DASS family sulfate transporter (YP_003577054) was identified and characterized in the Alphaproteobacterium R. capsulatum ( Gisin et al, 2010 ; Hoffmann et al, 2017 ). Recently, more DASS family sulfate transporters were characterized in the assimilatory sulfate-reducing Alphaproteobacteria Rhodobacter sphaeroides , Sinorhizobium melioti , Agrobacterium tumefaciens , Dinoroseobacter shibae and the gammaproteobacterium Pseudomonas stutzeri ( Hoffmann et al, 2017 ). Contrary to the characterized eukaryotic DASS sulfate transporters the prokaryotic homologues exhibited variable transport efficiency and overall they appeared to function as low affinity transporters ( Gisin et al, 2010 ; Hoffmann et al, 2017 ).…”

Section: Sulfate Transporters In Dissimilatory Sulfate Reducing Bactementioning

confidence: 99%

“…Recently, more DASS family sulfate transporters were characterized in the assimilatory sulfate-reducing Alphaproteobacteria Rhodobacter sphaeroides , Sinorhizobium melioti , Agrobacterium tumefaciens , Dinoroseobacter shibae and the gammaproteobacterium Pseudomonas stutzeri ( Hoffmann et al, 2017 ). Contrary to the characterized eukaryotic DASS sulfate transporters the prokaryotic homologues exhibited variable transport efficiency and overall they appeared to function as low affinity transporters ( Gisin et al, 2010 ; Hoffmann et al, 2017 ). Variable sulfate transport affinity by DASS-family putative sulfate transporters was also observed in the sulfate reducer Desulfobacterium autotrophicum where the DASS family transporter HRM2_38300 was preferentially expressed in the presence of excess sulfate (mM), while the DASS family transporter HRM2_40290 was preferentially expressed in the presence of less than 100 μM sulfate ( Tarpgaard et al, 2017 ).…”

Section: Sulfate Transporters In Dissimilatory Sulfate Reducing Bactementioning

confidence: 99%

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Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms: A Comparative Genomics Analysis

et al. 2018

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The first step in the sulfate reduction pathway is the transport of sulfate across the cell membrane. This uptake has a major effect on sulfate reduction rates. Much of the information available on sulfate transport was obtained by studies on assimilatory sulfate reduction, where sulfate transporters were identified among several types of protein families. Despite our growing knowledge on the physiology of dissimilatory sulfate-reducing microorganisms (SRM) there are no studies identifying the proteins involved in sulfate uptake in members of this ecologically important group of anaerobes. We surveyed the complete genomes of 44 sulfate-reducing bacteria and archaea across six phyla and identified putative sulfate transporter encoding genes from four out of the five surveyed protein families based on homology. We did not find evidence that ABC-type transporters (SulT) are involved in the uptake of sulfate in SRM. We speculate that members of the CysP sulfate transporters could play a key role in the uptake of sulfate in thermophilic SRM. Putative CysZ-type sulfate transporters were present in all genomes examined suggesting that this overlooked group of sulfate transporters might play a role in sulfate transport in dissimilatory sulfate reducers alongside SulP. Our in silico analysis highlights several targets for further molecular studies in order to understand this key step in the metabolism of SRMs.

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Section: Sulfate Transporters In Dissimilatory Sulfate Reducing Bactementioning

confidence: 99%

Section: Sulfate Transporters In Dissimilatory Sulfate Reducing Bactementioning

confidence: 99%

Section: Sulfate Transporters In Dissimilatory Sulfate Reducing Bactementioning

confidence: 99%

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Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms: A Comparative Genomics Analysis

et al. 2018

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“…YfbS is predicted to contain 12 transmembrane domains (TMDs), with two TrkA_C domains located within a large intracellular loop between TMDs 5 and 6 (Figure b). TrkA_C domains are predicted to bind ligands and are widespread in bacteria (Hoffmann et al., 2017). To date, PerO of the alphaproteobacterium Rhodobacter capsulatus is the best characterized member of a SLC13 family member that contains TrkA_C domains, and it functions as a low‐affinity oxyanion permease (Hoffmann et al., 2017).…”

Section: Resultsmentioning

confidence: 99%

Vibrio fischeri imports and assimilates sulfate during symbiosis with Euprymna scolopes

Wasilko

Ceron

Baker

et al. 2021

Molecular Microbiology

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Sulfur is in cellular components of bacteria and is, therefore, an element necessary for growth. However, mechanisms by which bacteria satisfy their sulfur needs within a host are poorly understood. Vibrio fischeri is a bacterial symbiont that colonizes, grows, and produces bioluminescence within the light organ of the Hawaiian bobtail squid, which provides an experimental platform for investigating sulfur acquisition in vivo. Like other γ‐proteobacteria, V. fischeri fuels sulfur‐dependent anabolic processes with intracellular cysteine. Within the light organ, the abundance of a ΔcysK mutant, which cannot synthesize cysteine through sulfate assimilation, is attenuated, suggesting sulfate import is necessary for V. fischeri to establish symbiosis. Genes encoding sulfate‐import systems of other bacteria that assimilate sulfate were not identified in the V. fischeri genome. A transposon mutagenesis screen implicated YfbS as a sulfate importer. YfbS is necessary for growth on sulfate and in the marine environment. During symbiosis, a ΔyfbS mutant is attenuated and strongly expresses sulfate‐assimilation genes, which is a phenotype associated with sulfur‐starved cells. Together, these results suggest V. fischeri imports sulfate via YfbS within the squid light organ, which provides insight into the molecular mechanisms by which bacteria harvest sulfur in vivo.

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“…An exception is the PerO anion permease of Rhodobacter capsulatus, which when inactivated increases resistance to molybdate, tungstate, and vanadate [51]. Moreover, sulfate transport and increased resistance were confirmed for five homologous permeases across the Proteobacteria [52]. PerO is a general, lowaffinity anion transporter-i.e.…”

Section: Discussionmentioning

confidence: 99%

Anion transport as a target of adaption to perchlorate in sulfate-reducing communities

et al. 2019

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Inhibitors can be used to control the functionality of microbial communities by targeting specific metabolisms. The targeted inhibition of dissimilatory sulfate reduction limits the generation of toxic and corrosive hydrogen sulfide across several industrial systems. Sulfate-reducing microorganisms (SRM) are specifically inhibited by sulfate analogs, such as perchlorate. Previously, we showed pure culture SRM adaptation to perchlorate stress through mutation of the sulfate adenylyltransferase, a central enzyme in the sulfate reduction pathway. Here, we explored adaptation to perchlorate across unconstrained SRM on a community scale. We followed natural and bio-augmented sulfidogenic communities through serial transfers in increasing concentrations of perchlorate. Our results demonstrated that perchlorate stress altered community structure by initially selecting for innately more resistant strains. Isolation, whole-genome sequencing, and molecular biology techniques allowed us to define subsequent genetic mechanisms of adaptation that arose across the dominant adapting SRM. Changes in the regulation of divalent anion:sodium symporter family transporters led to increased intracellular sulfate to perchlorate ratios, allowing SRM to escape the effects of competitive inhibition. Thus, in contrast to pure-culture results, SRM in communities cope with perchlorate stress via changes in anion transport and its regulation. This highlights the value of probing evolutionary questions in an ecological framework, bridging the gap between ecology, evolution, genomics, and physiology.

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Bacterial PerO Permeases Transport Sulfate and Related Oxyanions

Cited by 9 publications

References 38 publications

Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms: A Comparative Genomics Analysis

Sulfate Transporters in Dissimilatory Sulfate Reducing Microorganisms: A Comparative Genomics Analysis

Vibrio fischeri imports and assimilates sulfate during symbiosis with Euprymna scolopes

Anion transport as a target of adaption to perchlorate in sulfate-reducing communities

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