Bacterial Penicillin Amidase

Claridge, C. A.; Gourevitch, A.; Lein, Joseph

doi:10.1038/187237a0

Cited by 96 publications

(31 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Point mutations in PBPs reduce the affinity for penicillin, which may increase penicillin resistance [47]. Additionally, penicillin resistance may be the consequence of penicillin acylase activity [48], which has been reported in many species of Gram-negative bacteria [49–51], leading to the hydrolysis of penicillin, with the production of the relatively inactive 6-aminopenicillanic acid. Another common mechanism underlying penicillin resistance is the activity of β-lactamase, which hydrolyzes the β-lactam ring and, consequently, deactivates β-lactam antibiotics [52].…”

Section: Resultsmentioning

confidence: 99%

Genomic analysis of the original Elberg Brucella melitensis Rev.1 vaccine strain reveals insights into virulence attenuation

2018

View full text Add to dashboard Cite

The live attenuated Brucella melitensis Rev.1 Elberg-originated vaccine strain has been widely used to control brucellosis in small ruminants. However, despite extensive research, the molecular mechanisms underlying the attenuation of this strain are still unknown. In the current study, we conducted a comprehensive comparative analysis of the whole-genome sequence of Rev.1 against that of the virulent reference strain, B. melitensis 16M. This analysis revealed five regions of insertion and three regions of deletion within the Rev.1 genome, among which, one large region of insertion, comprising 3,951 bp, was detected in the Rev.1 genome. In addition, we found several missense mutations within important virulence-related genes, which may be used to determine the mechanism underlying virulence attenuation. Collectively, our findings provide new insights into the Brucella virulence mechanisms and, therefore, may serve as a basis for the rational design of new Brucella vaccines.

show abstract

Section: Resultsmentioning

confidence: 99%

Genomic analysis of the original Elberg Brucella melitensis Rev.1 vaccine strain reveals insights into virulence attenuation

2018

View full text Add to dashboard Cite

show abstract

“…A process for the manufacture of 6-APA by deacylation of penicillin was first developed in our laboratories using a deacylase obtained from Streptomyces lavendulae with penicillin V as substrate; a patent for this process was filed in March 1959. Subsequently the deacylase of bacterial origin was discovered independently in a number of laboratories [6][7][8][9] and a process for 6-APA production was then developed using this type of enzyme with penicillin G as substrate. With the availability of 6-APA and the opportunity to prepare semisynthetic penicillins, a number of objectives presented themselves.We have studied the theoretical investigation on the 6-APA and zwitterion of 6-APA ( Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Theoretical Investigation of Structural, Thermodynamical and HOMO, LUMO Analysis of 6-Aminopenicillanic Acid

2017

Chem Sci Trans

View full text Add to dashboard Cite

Quantum chemical calculations using density functional theory and 6-311G (d) basis set have been applied to analyze the interactions of β-lactam compounds and the electronic structural properties including thermochemical parameters have been investigated. The present investigation is extended to calculate the HOMO-LUMO energy gap. The calculated HOMO-LUMO energy gap shows that the charge transfer occurs within the molecule. The frontier orbital and molecular electrostatic potential surface studies have been employed to understand the active structure. The results presented in this article will be helpful to improve existing model and will open up a whole new arena of study to understand the antimicrobial characteristic from natural products against the photosensitizing DNA damage induce by 6-aminopenicillanic acid extracts and enable amuch clearer understanding of how antimicrobial drugs mediate their effects on the specific spot.

show abstract

“…However, the occurrence of penicillin acylase activity, leading to the hydrolysis of penicillins with the production of the relatively inactive 6-aminopenicillanic acid (6-APA) ( Fig. 1) has been reported in many species of Gram-negative bacteria Rolinson et al 1960;Claridge, Gourevitch & Lein, 1960;Huang, Seto & Shull, 1963), and resistance to penicillins as a result of penicillin acylase activity has been described by English, McBride & Huang (1960) and by Holt & Stewart (1964~). The latter authors reported that 40% of 310 clinical strains of Escherichia coli examined produced penicillin amidase (penicillin acylase), but the properties of the enzyme extracted from one of these cultures, E. coli NCIB 9465, (Holt & Stewart, 1964b) differed very markedly from those of the penicillin acylases produced by other strains of E. coli (Rolinson et al 1960;Kaufmann, Bauer & Offe, 1960;Kaufmann & Bauer, 1964).…”

Section: Introductionmentioning

confidence: 99%

The Role of Penicillin Acylase in the Resistance of Gram-Negative Bacteria to Penicillins

Cole¹,

Sutherland²

1966

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARYThe resistance of 148 clinical isolates of Gram-negative bacteria to ampicillin and benzylpenicillin was determined in serial-dilution sensitivity tests, together with the extent of inactivation of the penicillins, and the production of 6-aminopenicillanic acid (6-APA). Many of the cultures were resistant to the penicillins and inactivated the compounds, but only one culture, a strain of Escherichia coli, showed penicillin acylase activity, as indicated by the production of 6-APA. However, the cultural conditions prevailing in serial-dilution tests, namely, stationary culture at 37O, although favourable for the functioning of penicillin acylases, were shown to be highly unfavourable for production of the enzyme by coliform bacteria.

show abstract

Bacterial Penicillin Amidase

Cited by 96 publications

References 2 publications

Genomic analysis of the original Elberg Brucella melitensis Rev.1 vaccine strain reveals insights into virulence attenuation

Genomic analysis of the original Elberg Brucella melitensis Rev.1 vaccine strain reveals insights into virulence attenuation

Theoretical Investigation of Structural, Thermodynamical and HOMO, LUMO Analysis of 6-Aminopenicillanic Acid

The Role of Penicillin Acylase in the Resistance of Gram-Negative Bacteria to Penicillins

Contact Info

Product

Resources

About