Bacterial pathogens from Diprion pini L. (Hymenoptera: Diprionidae) and their biocontrol potential

Çelik, Tayyib; Sevim, Ali

doi:10.1007/s11756-022-01161-0

Cited by 2 publications

(2 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enterobacter cancerogenus is a Gram-negative anaerobe bacillus [1,2]. It is widely distributed in nature [3], and has been successfully isolated from insect Diprion pini (Hymenoptera, Diprionidae) [4] and Lutzomyia evansi (Diptera, Psychodidae) [5] and plant tomato (Solanum lycopersicum) [6] and rice (Oryza sativa) [7]. A few reports suggest that E. cancerogenus may be a potential pathogen infecting patient skin and soft tissues when a wound exists and cause human diseases such as sepsis [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…This was the first isolated strain belonging to E. cancerogenus to have high insecticidal activity against insect larvae. Excitingly, some strains of Enterobacter showed insecticidal, acaricidal, nematocidal, fungicidal, and plant growth-promoting activities, indicating that Enterobacter bacteria have biological control potential in agriculture [4,12,13]. Moreover, some strains contain type VI secretion systems (T6SS), and can establish competition by secreting antimicrobial proteins in the periplasm of bacterial targets [14].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reference Genes for Expression Analyses by qRT-PCR in Enterobacter cancerogenus

Pan,

Zhao,

Zeng

et al. 2024

Microorganisms

View full text Add to dashboard Cite

The Enterobacter cancerogenus strain EcHa1 was isolated from the dead larvae of Helicoverpa armigera, and has the potential for biocontrol of some Lepidoptera insects. In order to screen insecticidal-related genes by qRT-PCR, stable endogenous reference genes used for normalizing qRT-PCR data were selected and evaluated from 13 housekeeping genes (HKGs). The expression levels of the HKGs were determined using qRT-PCR under different experimental conditions, including two culture temperatures and three bacterial OD values. Five stability analysis methods (Ct, BestKeeper, NormFinder, geNorm, and RefFinder) were used to comprehensively rank the candidate genes. The results showed that the optimal reference genes varied under different experimental conditions. The combination of gyrA and gyrB was recommended as the best reference gene combination at 28 °C, while gyrA and rpoB was the best combination at 37 °C. When the OD values were 0.5, 1.0 and 2.0, the recommended reference gene combinations were ftsZ and gyrA, rpoB and gyrB, and gyrA and pyk, respectively. The most suitable reference genes were gyrA and gyrB under all experimental conditions. Using gyrA and gyrB as the reference genes for qRT-PCR, EcHa1 was found to invade all tissues of the H. armigera larvae, and expressed a candidate pathogenic factor Hcp at high levels in gut, Malpighian tubules, and epidermis tissues. This study not only establishes an accurate and reliable normalization for qRT-PCR in entomopathogenic bacteria but also lays a solid foundation for further study of functional genes in E. cancerogenus.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reference Genes for Expression Analyses by qRT-PCR in Enterobacter cancerogenus

Pan,

Zhao,

Zeng

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

Malacosoma castrensis ile İlişkili Kültüre Edilebilir Bağırsak Bakteri Toplulukları: İzolasyon ve 16S rRNA Dizin Analizi ile Tanımlama

SEVİM

2023

Nevşehir Bilim Ve Teknoloji Dergisi

View full text Add to dashboard Cite

Pek çok hayvan ve bitki vücutlarının içerisinde simbiyotik mikroorganizmalara sahiptir ve bu ortaklar arasında yakın etkileşimler meydana gelmektedir. Böcekler ise en büyük hayvan grubu oluşturmaktadırlar ve zorunlu mutualizm’den fakültatif parazitizm’e kadar değişen çeşitli endosimbiyotik ilişkileri içermektedirler. Böceklerdeki endosimbiyotik bakterilerin beslenme, üreme, savunma, iletişim, davranış ve gelişim gibi pek çok farklı rolleri bulunmaktadır. Bu çalışmada böceklerdeki simbiyotik bakterileri tanımlamak için model organizma olarak Malacosoma castrensis seçilmiştir. Bu böceğin larvalarında yer alan bakteriler izole edilmiş ve 16S rRNA sekans analizi ile tanımlanmıştır. Toplam olarak 6 adet bakteri izole edilmiş ve bunlar Staphylococcus sp. MM-1, Micrococcus sp. MM-2, Rhodococcus sp. MM-3, Arthrobacter citreus MM-4, Bacillus sp. MM-5 ve Pseudomonas sp. MM-6 olarak tanımlanmıştır. Elde edilen sonuçların böcek-bakteri ilişkilerinin belirlenmesinde ve böceklerdeki endosimbiyotik bakterilerinin rollerinin aydınlatılmasında faydalı olacağı düşünülmektedir.

show abstract

Bacterial pathogens from Diprion pini L. (Hymenoptera: Diprionidae) and their biocontrol potential

Cited by 2 publications

References 74 publications

Reference Genes for Expression Analyses by qRT-PCR in Enterobacter cancerogenus

Reference Genes for Expression Analyses by qRT-PCR in Enterobacter cancerogenus

Malacosoma castrensis ile İlişkili Kültüre Edilebilir Bağırsak Bakteri Toplulukları: İzolasyon ve 16S rRNA Dizin Analizi ile Tanımlama

Contact Info

Product

Resources

About