Bacterial pathogens associated with lung lesions in slaughter pigs from 125 herds

Fablet, C.; Marois, C.; Dorenlor, V.; Eono, F.; Éveno, É.; Jolly, J. P.; Devendec, Laëtitia Le; Kobisch, Marylène; Madec, F.; Rose, Nicolas

doi:10.1016/j.rvsc.2011.11.002

Cited by 65 publications

(76 citation statements)

References 15 publications

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“…PRRS virus is exotic in Brazil. Pasteurella multocida (P. multocida) appears as the most common bacterial agent in PRDC related to pleuropneumonia cases (Morés 2006, Hansen et al 2010, Fablet et al 2011.…”

Section: Introductionmentioning

confidence: 99%

“…Historically, P. multocida is considered to be a secondary infection, aggravating pulmonary infections caused by M. hyopneumoniae and PCV2 (Kim et al 2003, Pijoan 2006, Hansen et al 2010, Wellenberg et al 2010, Fablet et al 2011, Pors et al 2011. However, researchers in southern Brazil (Borowski 2001, Morés 2006, Borowski et al 2007, Kich et al 2007 and Argentina (Cappuccio et al 2004) have suggested that P. multocida is a primary agent capable of causing severe respiratory disease, nevertheless its pathogenesis and invasiveness mechanisms are not clearly described yet.…”

Section: Introductionmentioning

confidence: 99%

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Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

et al. 2017

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The bacterium Pasteurella multocida is a frequent cause of porcine respiratory disease complex in finishing pigs. Historically, the bacterium is recognized as an opportunistic agent, causing secondary bacterial pneumonia in pigs. Several Brazilian reports have suggested the ability of P. multocida to cause primary pulmonary infection that leads to the death of finishing pigs prior to slaughter. The aim of this study was to evaluate anatomopathological pulmonary findings associated with P. multocida infection that were obtained from animals with clinical respiratory disease and from animals at slaughter. Twenty-five lung samples from 14 herds of finishing pigs with acute clinical respiratory disease and 19 lungs collected at slaughter from a different set of 14 herds were studied. In all lung samples, bacterial isolation was performed, and only samples with pure P. multocida growth were included in the study. Gross and histopathological lesions were evaluated, as well as Influenza A, porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae co-infections. Pleuritis and pericarditis were more often observed in clinical samples (P<0.05). Moreover, there was a numerical trend indicating that pericarditis, lymphadenomegaly and cavity exudates were more often present in clinical samples. Thirteen lung samples were negative to M. hyopneumoniae, Influenza A and PCV2 by immunohistochemistry (IHC), with only P. multocida identified. In these cases, gross lesions such as pleuritis, pericarditis and lymphadenomegaly were always present, and no histologic lesions indicative of other agents such as Actinobacillus pleuropneumoniae, Actinobacillus suis or Haemophilus parasuis were observed. These findings suggest the ability of some P. multocida isolates to cause primary respiratory and systemic infection. However, in this study, it was not possible to determine specific virulence markers to explain these findings.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

et al. 2017

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“…In the first of the case, there is little information about their presence in Mexico. PRRSV is currently considered to be the most significant and economically important infectious disease to afflict swine worldwide [14] [33] [56] [57]. In the case of samples from water, existing reports that PRRSV can be isolated from water are kept at 25˚C -27˚C for up to 11 days [58], and in swine lagoon effluent kept at 4˚C for 8 days [59].…”

Section: Discussionmentioning

confidence: 99%

Porcine Respiratory Pathogens in Swine Farms Environment in Mexico

Loera-Muro¹,

Loera‐Muro²,

Morfín-Mata³

et al. 2014

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Respiratory pathogens are the main health problem in the swine industry worldwide. These pathogens are transmitted by direct contact between animals or by aerosols and however are not well known yet, if the environment works as its reservoir, inoculum and/or dispersion medium. The objective of this study was to determine the presence of respiratory pathogens in environmental samples from swine farms in Aguascalientes, Mexico, through of PCR and RT-PCR techniques. The bacteria Actinobacillus pleuropneumoniae and Pasteurella multocida were found viable in samples from water, food, soil and air. Streptococcus suis was found in a viable state in water samples. Haemophilus parasuis, Porcine Reproductive and Respiratory Syndrome virus and Swine Influenza virus (H1N1 and H3N2) were detected in drinking water samples. Mycoplasma hyopneumoniae and Porcine Circovirus type 2 (PCV2) were not detected in environmental samples. These results suggest that the environment of the farms acts as a reservoir, inoculum and/or vehicle of dispersion for these pathogens except for M. hyopneumoniae and PCV2.

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“…Chan et al (2014) obtiveram em amostras de PCR amplificado para genes de resistên-cia àà meticilina em Staphylococcus aureus um valor para limite de detecção das AuNPs de 500ng e Hussain et al (2013) (Hansen et al 2010, Fablet et al 2012) e ainda os microrganismos Mycoplasma hyopneumoniae e Streptococcus suis, a escolha dos oligonucleotídeos e dos genes alvos para os testes pode diminuir essas reações cruzadas.…”

Section: Discussão E Conclusãounclassified

Padronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos

et al. 2014

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Standardization of unmodified gold nanoparticle (AuNPs) for detection of Actinobacillus pleuropneumoniae in swine lungs.] Padronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos. Pesquisa Veterinária Brasileira 34(7):621-625. Laboratório de Microbiologia e Biologia Molecular, Universidade Federal do Mato Grosso, Av. Fernando Corrêa da Costa2367, Bairro Boa Esperança, Cuiabá, MT 78060-900, Brazil. E-mail: valdutra@ufmt.br Based on diagnostic tests for the detection of nucleic acids without amplification through the use of gold nanoparticles (AuNPs) have been described for various diseases. This study aimed to develop a technique of unmodified AuNPs to detect Actinobacillus pleuropneumoniae (App). We used 70 lung samples from pigs, 17 with and 53 without characteristic lesions of pneumonia, to detect App. The primer used was based on ApxIV gene. The AuNPs test had a sensitivity of 93.8% and specificity of 84.6% when compared with PCR detection. The results showed good agreement between AuNPs and PCR testing, and the technique can be used as an alternative to conventional tests, since it is quick and easy, and does not require implementation infrastructure and skilled labor.INDEX TERMS: Swine pneumonia, gold nanoparticle, AuNP, Actinobacillus pleuropneumoniae. (Rossi et al. 2013). São consideradas doenças multifatoriais, provocando significativas perdas econômicas, geralmente associadas à produção intensiva, a fatores ambientais e de manejo (Hansen et al. 2010, Opriessnig, Gimenez-Lirola & Halbur 2011. O diagnóstico baseia-se no isolamento do agente a partir de lesões suspeitas e, principalmente, em testes sorológicos para estabelecimento de medidas de controle (Coelho et al. 2004, Xie et al. 2013.A pleuropneumonia suína (PPS) causada pela bactéria Actinobacillus pleuropneumoniae (App), provoca doença clí-nica caracterizada por pneumonia com pleurisia fibrinosa, lesões pulmonares necrohemorrágicas, adesões pleurais fibrinóticas e, em casos severos, à morte (Bossé et al. 2002). O contágio geralmente ocorre após inalação de aerossóis ou contato direto, o microrganismo inalado coloniza o tecido pulmonar aderindo-se ao muco, proteínas e a células hospedeiras com posterior multiplicação no local (Chiers et al. 2010). O monitoramento do status da doença em rebanhos, o controle e a erradicação são de fundamental importância devido a características de contágio rápido deste agente, para impedir que animais portadores sejam introduzidos em rebanhos saudáveis (Tremblay et al. 2013).Testes indiretos são muito utilizados (Machado et al. 2001, Shin et al. 2011, Eamens et al. 2012a,b, GimenezLirola et al. 2014,para detecção simultânea de anticorpos para toxinas com sensibilidade de 82,7% e especificidade de 100% (Gimenez-Lirola et al. 2014). São descritos testes de ELISA monovalentes e polivalentes para sorotipos, com sensibilidade e especificidade variando de 88,3% a 96,6% (Machado et al. 2001). A importância de alta sensibilidade e especific...

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Bacterial pathogens associated with lung lesions in slaughter pigs from 125 herds

Cited by 65 publications

References 15 publications

Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

Anatomopathological pneumonic aspects associated with highly pathogenic Pasteurella multocida in finishing pigs

Porcine Respiratory Pathogens in Swine Farms Environment in Mexico

Padronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos

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